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Hydrogen sulfide promotes nodulation and nitrogen fixation in soybean-rhizobia symbiotic system

Hang Zou, Ni-Na Zhang, Qing Pan, Jian-Hua Zhang, Juan Chen, Ge-Hong Wei
doi: https://doi.org/10.1101/367896
Hang Zou
1State Key Laboratory of Crop Stress Biology in Arid Areas, College of life sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China
2Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, Yangling Shaanxi 712100, PR China
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Ni-Na Zhang
3State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China
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Qing Pan
1State Key Laboratory of Crop Stress Biology in Arid Areas, College of life sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China
2Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, Yangling Shaanxi 712100, PR China
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Jian-Hua Zhang
4School of Life Sciences and State Key Laboratory of Agrobiotechnology, the Chinese University of Hong Kong, Hong Kong
5Department of Biology, Hong Kong Baptist University, Hong Kong
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Juan Chen
1State Key Laboratory of Crop Stress Biology in Arid Areas, College of life sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China
3State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China
4School of Life Sciences and State Key Laboratory of Agrobiotechnology, the Chinese University of Hong Kong, Hong Kong
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  • For correspondence: chenjuan@nwsuaf.edu.cn weigehong@nwsuaf.edu.cn
Ge-Hong Wei
1State Key Laboratory of Crop Stress Biology in Arid Areas, College of life sciences, Northwest A&F University, Yangling, Shaanxi 712100, PR China
2Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, Yangling Shaanxi 712100, PR China
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  • For correspondence: chenjuan@nwsuaf.edu.cn weigehong@nwsuaf.edu.cn
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Abstract

The rhizobium-legume symbiotic system is crucial for nitrogen cycle balance in agriculture. Hydrogen sulfide (H2S), a gaseous signaling molecule, may regulate various physiological processes in plants. However, whether H2S has regulatory effect in this symbiotic system remains unknown. Herein, we investigated the possible role of H2S in the symbiosis between soybean (Glycine max) and rhizobium (Sinorhizobium fredii). Our results demonstrated that exogenous H2S donor (sodium hydrosulfide, NaHS) treatment promoted soybean growth, nodulation and nitrogenase (Nase) activity. Western blotting analysis revealed that the abundance of nitrogenase component nifH was increased by NaHS treatment in nodules. Quantitative real-time PCR data showed that NaHS treatment up-regulated the expressions of symbiosis-related genes nodC and nodD of S. fredii. Besides, expression of soybean nodulation marker genes including early nodulin 40 (GmENOD40), ERF required for nodulation (GmERN), nodulation signaling pathway2b (GmNSP2b) and nodulation inception genes (GmNIN1a, GmNIN2a and GmNIN2b) were up-regulated. Moreover, the expressions of glutamate synthase (GmGS), nitrite reductase (GmNiR), ammonia transporter (GmSAT1), and nifH involved in nitrogen metabolism were up-regulated in NaHS-treated soybean roots and nodules. Together, our results suggested that H2S may act as a positive signaling molecule in soybean-rhizobia symbiotic system and enhance their nitrogen fixation ability.

Highlight We demonstrated for the first time that H2S as a signaling molecule may promote the establishment of symbiotic relationship and nitrogen fixation ability in the soybean-rhizobia symbiotic system.

Abbreviations
Ci
carbon dioxide concentration.
Fm
maximal fluorescence yield.
Fo
minimal fluorescence yield.
Gs
stomatal conductance.
Pn
photosynthetic rate.
PSII
photosystem II.
qRT-PCR
quantitative real-time PCR.
Tr
transpiration rate.
Vpdl
vapor pressure divicit.

Footnotes

  • zouhang1108{at}126.com, zhaangnina{at}126.com, Sunny2009608{at}outlook.com, jzhang{at}hkbu.edu.hk, chenjuan{at}nwsuaf.edu.cn, weigehong{at}nwsuaf.edu.cn

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Posted July 12, 2018.
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Hydrogen sulfide promotes nodulation and nitrogen fixation in soybean-rhizobia symbiotic system
Hang Zou, Ni-Na Zhang, Qing Pan, Jian-Hua Zhang, Juan Chen, Ge-Hong Wei
bioRxiv 367896; doi: https://doi.org/10.1101/367896
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Hydrogen sulfide promotes nodulation and nitrogen fixation in soybean-rhizobia symbiotic system
Hang Zou, Ni-Na Zhang, Qing Pan, Jian-Hua Zhang, Juan Chen, Ge-Hong Wei
bioRxiv 367896; doi: https://doi.org/10.1101/367896

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