Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Posttranscriptional regulation of intestinal epithelial cell repair by RNA binding protein IMP1

Priya Chatterji, Kelly A. Whelan, Sarah F. Andres, View ORCID ProfileFernando C. Samper, Lauren A. Simon, Rei Mizuno, Emma T. Lundsmith, David S.M. Lee, Shun Liang, H.R. Sagara Wijeratne, Stefanie Marti, Lillian Chau, Patrick A. Williams, Veronique Giroux, Benjamin J. Wilkins, Gary D. Wu, View ORCID ProfilePremal Shah, View ORCID ProfileGian G. Tartaglia, Kathryn E. Hamilton
doi: https://doi.org/10.1101/368050
Priya Chatterji
1Division of Gastroenterology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kelly A. Whelan
2Department of Pathology & Laboratory Medicine, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, 19140, USA;
3Fels Institute for Cancer Research & Molecular Biology, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, 19140, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sarah F. Andres
1Division of Gastroenterology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fernando C. Samper
4Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain;
5Universitat Pompeu Fabra (UPF), Barcelona, Spain;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Fernando C. Samper
Lauren A. Simon
6Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Children’s Hospital of Philadelphia, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rei Mizuno
1Division of Gastroenterology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Emma T. Lundsmith
7Thomas Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, 19107, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David S.M. Lee
8Genomics and Computational Biology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shun Liang
9Department of Genetics; Rutgers University, New Brunswick, NJ, 08901, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
H.R. Sagara Wijeratne
9Department of Genetics; Rutgers University, New Brunswick, NJ, 08901, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stefanie Marti
4Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain;
5Universitat Pompeu Fabra (UPF), Barcelona, Spain;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lillian Chau
1Division of Gastroenterology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Patrick A. Williams
6Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Children’s Hospital of Philadelphia, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Veronique Giroux
1Division of Gastroenterology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin J. Wilkins
10Department of Pathology and Laboratory Medicine, Children’s Hospital of Philadelphia, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gary D. Wu
1Division of Gastroenterology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Premal Shah
9Department of Genetics; Rutgers University, New Brunswick, NJ, 08901, USA;
11Human Genetics Institute of New Jersey, Piscataway, NJ, 08854, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Premal Shah
Gian G. Tartaglia
4Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain;
5Universitat Pompeu Fabra (UPF), Barcelona, Spain;
12Institucio Catalana de Recerca i Estudis Avanc ats (ICREA), Barcelona, Spain.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gian G. Tartaglia
Kathryn E. Hamilton
6Division of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Children’s Hospital of Philadelphia, Philadelphia, PA, 19104, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

RNA binding proteins, such as IMP1, are emerging as essential regulators of intestinal development and cancer. IMP1 hypomorphic mice exhibit severe intestinal growth defects, yet it’s role in adult intestinal epithelium is unclear. We employed ribosome profiling to test the effect of IMP1 loss on the “translatome” in colon cancer cell lines. In parallel, we evaluated mice with intestinal epithelial-specific Imp1 deletion (Imp1ΔIEC) following irradiation or colitis models. Ribosome-profiling revealed translation efficiency changes for multiple pathways important for intestinal homeostasis, including autophagy, in IMP1 knockout cells. We found increased autophagy flux in Imp1ΔIEC mice, reinforced through in silico and biochemical analyses revealing direct binding of IMP1 to autophagy transcripts MAP1LC3B and ATG3. We found that Imp1ΔIEC mice exhibit enhanced recovery following irradiation, which is attenuated with genetic deletion of autophagy gene Atg7. Finally, we demonstrated that IMP1 is upregulated in Crohn’s disease patients and Imp1 loss lessened colitis severity in mice. These studies demonstrate that IMP1 acts as a posttranscriptional regulator of gut epithelial repair post-irradiation and colitis, in part through modulation of autophagy.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted July 12, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Posttranscriptional regulation of intestinal epithelial cell repair by RNA binding protein IMP1
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Posttranscriptional regulation of intestinal epithelial cell repair by RNA binding protein IMP1
Priya Chatterji, Kelly A. Whelan, Sarah F. Andres, Fernando C. Samper, Lauren A. Simon, Rei Mizuno, Emma T. Lundsmith, David S.M. Lee, Shun Liang, H.R. Sagara Wijeratne, Stefanie Marti, Lillian Chau, Patrick A. Williams, Veronique Giroux, Benjamin J. Wilkins, Gary D. Wu, Premal Shah, Gian G. Tartaglia, Kathryn E. Hamilton
bioRxiv 368050; doi: https://doi.org/10.1101/368050
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Posttranscriptional regulation of intestinal epithelial cell repair by RNA binding protein IMP1
Priya Chatterji, Kelly A. Whelan, Sarah F. Andres, Fernando C. Samper, Lauren A. Simon, Rei Mizuno, Emma T. Lundsmith, David S.M. Lee, Shun Liang, H.R. Sagara Wijeratne, Stefanie Marti, Lillian Chau, Patrick A. Williams, Veronique Giroux, Benjamin J. Wilkins, Gary D. Wu, Premal Shah, Gian G. Tartaglia, Kathryn E. Hamilton
bioRxiv 368050; doi: https://doi.org/10.1101/368050

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Physiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3703)
  • Biochemistry (7828)
  • Bioengineering (5705)
  • Bioinformatics (21362)
  • Biophysics (10611)
  • Cancer Biology (8217)
  • Cell Biology (11982)
  • Clinical Trials (138)
  • Developmental Biology (6792)
  • Ecology (10430)
  • Epidemiology (2065)
  • Evolutionary Biology (13916)
  • Genetics (9734)
  • Genomics (13116)
  • Immunology (8181)
  • Microbiology (20081)
  • Molecular Biology (7882)
  • Neuroscience (43201)
  • Paleontology (321)
  • Pathology (1285)
  • Pharmacology and Toxicology (2270)
  • Physiology (3367)
  • Plant Biology (7262)
  • Scientific Communication and Education (1317)
  • Synthetic Biology (2012)
  • Systems Biology (5551)
  • Zoology (1135)