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Tailoring 3D cell culture templates with common hydrogels

Aurélien Pasturel, Pierre-Olivier Strale, View ORCID ProfileVincent Studer
doi: https://doi.org/10.1101/370882
Aurélien Pasturel
1University of Bordeaux, Interdisciplinary Institute for Neuroscience, Bordeaux, France
2CNRS UMR 5297, F-33000 Bordeaux, France
3ALVEOLE, Paris, France
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Pierre-Olivier Strale
3ALVEOLE, Paris, France
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Vincent Studer
1University of Bordeaux, Interdisciplinary Institute for Neuroscience, Bordeaux, France
2CNRS UMR 5297, F-33000 Bordeaux, France
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Abstract

3D cell culture aims at reconciliating the simplicity of in vitro models with the human like properties encountered in vivo. Soft permeable hydrogels have emerged as user-friendly materials to grow cells in more physiological conditions. With the intent on turning these homogeneous substrates into biomimetic templates, we introduce a generic solution compatible with the most biologically relevant and often frail materials. Here we take control of the chemical environment driving generic radical reactions to craft common gels with patterned light. In a simple microreactor, we harness the well-known inhibition of radicals by oxygen to enable topographical photopolymerization. Strikingly, by sustaining an oxygen rich environment, we can also induce hydrogel photo-scission which turns out to be a powerful and generic subtractive manufacturing method. We finally introduce a flexible patterned functionalization protocol based on available photo-linkers. Using these common tools on the most popular hydrogels, we tailored soft templates where cells grow or self-organize into standardized structures. The platform we describe has the potential to set a standard in future 3D cell culture experiments.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted March 04, 2019.
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Tailoring 3D cell culture templates with common hydrogels
Aurélien Pasturel, Pierre-Olivier Strale, Vincent Studer
bioRxiv 370882; doi: https://doi.org/10.1101/370882
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Tailoring 3D cell culture templates with common hydrogels
Aurélien Pasturel, Pierre-Olivier Strale, Vincent Studer
bioRxiv 370882; doi: https://doi.org/10.1101/370882

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