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Radial F-actin Organization During Early Neuronal Development

Durga Praveen Meka, Robin Scharrenberg, Bing Zhao, Theresa König, Irina Schaefer, Birgit Schwanke, Oliver Kobler, Sergei Klykov, Melanie Richter, Dennis Eggert, Sabine Windhorst, Carlos G. Dotti, Michael R. Kreutz, Marina Mikhaylova, Froylan Calderon de Anda
doi: https://doi.org/10.1101/372813
Durga Praveen Meka
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Robin Scharrenberg
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Bing Zhao
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Theresa König
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Irina Schaefer
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Birgit Schwanke
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Oliver Kobler
2Combinatorial Neuroimaging Core Facility (CNI), Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany.
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Sergei Klykov
3Emmy-Noether Group “Neuronal Protein Transport”, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Melanie Richter
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Dennis Eggert
4Max Planck Institute for the Structure and Dynamics of Matter, 22761 Hamburg and Heinrich Pette Institute - Leibniz Institute for Experimental Virology, 20251 Hamburg, Germany.
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Sabine Windhorst
5Department of Biochemistry and Signal Transduction, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany.
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Carlos G. Dotti
6Centro de Biología Molecular “Severo Ochoa”, CSIC-UAM, Madrid, Spain.
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Michael R. Kreutz
7RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany.
8Leibniz Guest Group “Dendritic Organelles and Synaptic Function”, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Marina Mikhaylova
3Emmy-Noether Group “Neuronal Protein Transport”, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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Froylan Calderon de Anda
1RG Neuronal Development, Center for Molecular Neurobiology Hamburg (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
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  • For correspondence: froylan.calderon@zmnh.uni-hamburg.de
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Abstract

The centrosome is thought to be the major neuronal microtubule-organizing center (MTOC) in early neuronal development, producing microtubules with a radial organization. In addition, albeit in vitro, recent work showed that isolated centrosomes could serve as an actin-organizing center (Farina et al., 2016), raising the possibility that neuronal development may, in addition, require a centrosome-based actin radial organization. Here we report, using super-resolution microscopy and live-cell imaging, F-actin organization around the centrosome with dynamic F-actin aster-like structures with F-actin fibers extending and retracting actively. Photoconversion/photoactivation experiments and molecular manipulations of F-actin stability reveal a robust flux of somatic F-actin towards the cell periphery. Finally, we show that somatic F-actin intermingles with centrosomal PCM-1 satellites. Knockdown of PCM-1 and disruption of centrosomal activity not only affect F-actin dynamics near the centrosome but also in distal growth cones. Collectively the data show a radial F-actin organization during early neuronal development, which might be a cellular mechanism for providing peripheral regions with a fast and continuous source of actin polymers; hence sustaining initial neuronal development.

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Posted December 12, 2018.
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Radial F-actin Organization During Early Neuronal Development
Durga Praveen Meka, Robin Scharrenberg, Bing Zhao, Theresa König, Irina Schaefer, Birgit Schwanke, Oliver Kobler, Sergei Klykov, Melanie Richter, Dennis Eggert, Sabine Windhorst, Carlos G. Dotti, Michael R. Kreutz, Marina Mikhaylova, Froylan Calderon de Anda
bioRxiv 372813; doi: https://doi.org/10.1101/372813
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Radial F-actin Organization During Early Neuronal Development
Durga Praveen Meka, Robin Scharrenberg, Bing Zhao, Theresa König, Irina Schaefer, Birgit Schwanke, Oliver Kobler, Sergei Klykov, Melanie Richter, Dennis Eggert, Sabine Windhorst, Carlos G. Dotti, Michael R. Kreutz, Marina Mikhaylova, Froylan Calderon de Anda
bioRxiv 372813; doi: https://doi.org/10.1101/372813

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