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Neural crest cells regulate optic cup morphogenesis by promoting extracellular matrix assembly

View ORCID ProfileChase D. Bryan, Rebecca L. Pfeiffer, Bryan W. Jones, Kristen M. Kwan
doi: https://doi.org/10.1101/374470
Chase D. Bryan
1Department of Human Genetics, University of Utah, Salt Lake City, UT, USA
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Rebecca L. Pfeiffer
2Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT, USA
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Bryan W. Jones
2Department of Ophthalmology and Visual Sciences, John A. Moran Eye Center, University of Utah School of Medicine, Salt Lake City, UT, USA
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Kristen M. Kwan
1Department of Human Genetics, University of Utah, Salt Lake City, UT, USA
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  • For correspondence: kmkwan@genetics.utah.edu
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Abstract

The interactions between an organ and its surrounding environment are critical in regulating its development. In vertebrates, neural crest and mesodermal mesenchymal cells have been observed close to the eye during development, and mutations affecting this periocular mesenchyme can cause defects in early eye development, yet the underlying mechanism has been unknown. Here, using timelapse microscopy and four-dimensional cell tracking in zebrafish, we establish that genetic loss of neural crest impairs cell movements within the optic vesicle. At the ultrastructural level, neural crest cells are required for basement membrane formation specifically around the retinal pigment epithelium. Neural crest cells express the extracellular matrix crosslinking protein nidogen and, strikingly, ectopically expressing nidogen in the absence of neural crest partially restores optic cup morphogenesis. These results demonstrate that the neural crest is required for local establishment of ocular extracellular matrix superstructure, which in turn drives optic cup morphogenesis.

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Posted July 22, 2018.
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Neural crest cells regulate optic cup morphogenesis by promoting extracellular matrix assembly
Chase D. Bryan, Rebecca L. Pfeiffer, Bryan W. Jones, Kristen M. Kwan
bioRxiv 374470; doi: https://doi.org/10.1101/374470
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Neural crest cells regulate optic cup morphogenesis by promoting extracellular matrix assembly
Chase D. Bryan, Rebecca L. Pfeiffer, Bryan W. Jones, Kristen M. Kwan
bioRxiv 374470; doi: https://doi.org/10.1101/374470

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