Abstract
Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules shows that high-quality 40-nm resolution images can be obtained in just tens of seconds.
Author Contributions
J.L. and S.H. designed experiments. J.L. and S.P. performed experiments and analyzed data. S.H. supervised the research. J.L. and S.H. wrote the paper.
Footnotes
Notes: The authors declare no competing financial interest.