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Modelling cancer immunomodulation using epithelial organoid cultures
View ORCID ProfileYotam E. Bar-Ephraim, View ORCID ProfileKai Kretzschmar, Priyanca Asra, Evelien de Jongh, Kim E. Boonekamp, View ORCID ProfileJarno Drost, Joost van Gorp, Apollo Pronk, Niels Smakman, Inez J. Gan, View ORCID ProfileZsolt Sebestyén, View ORCID ProfileJürgen Kuball, Robert G.J. Vries, View ORCID ProfileHans Clevers
doi: https://doi.org/10.1101/377655
Yotam E. Bar-Ephraim
1Oncode Institute, Hubrecht Institute, Royal Netherlands Academy for Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, the Netherlands
2UMC Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands
Kai Kretzschmar
1Oncode Institute, Hubrecht Institute, Royal Netherlands Academy for Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, the Netherlands
2UMC Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands
Priyanca Asra
1Oncode Institute, Hubrecht Institute, Royal Netherlands Academy for Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, the Netherlands
2UMC Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands
Evelien de Jongh
1Oncode Institute, Hubrecht Institute, Royal Netherlands Academy for Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, the Netherlands
2UMC Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands
Kim E. Boonekamp
1Oncode Institute, Hubrecht Institute, Royal Netherlands Academy for Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, the Netherlands
2UMC Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands
Jarno Drost
3Princess Máxima Centre for Paediatric Oncology, Utrecht, the Netherlands
Joost van Gorp
4Department of Pathology, Diakonessenhuis hospital, Utrecht, the Netherlands
Apollo Pronk
5Department of Surgery, Diakonessenhuis hospital, Utrecht, the Netherlands
Niels Smakman
5Department of Surgery, Diakonessenhuis hospital, Utrecht, the Netherlands
Inez J. Gan
6Laboratory of Translational Immunology, Department of Immunology, UMC, Utrecht, the Netherlands
Zsolt Sebestyén
6Laboratory of Translational Immunology, Department of Immunology, UMC, Utrecht, the Netherlands
Jürgen Kuball
6Laboratory of Translational Immunology, Department of Immunology, UMC, Utrecht, the Netherlands
Robert G.J. Vries
7Foundation Hubrecht Organoid Technology (HUB), Utrecht, the Netherlands
Hans Clevers
1Oncode Institute, Hubrecht Institute, Royal Netherlands Academy for Arts and Sciences (KNAW) and University Medical Centre (UMC) Utrecht, the Netherlands
2UMC Utrecht, Cancer Genomics Netherlands, Utrecht, the Netherlands
3Princess Máxima Centre for Paediatric Oncology, Utrecht, the Netherlands
Abstract
Here we utilize organoid technology to study immune-cancer interactions and assess immunomodulation by colorectal cancer (CRC). Transcriptional profiling and flow cytometry revealed that organoids maintain differential expression of immunomodulatory molecules present in primary tumours. Finally, we established a method to model antigen-specific epithelial cell killing and cancer immunomodulation in vitro using CRC organoids co-cultured with cytotoxic T cells (CTLs).
Copyright
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Posted October 04, 2018.
Modelling cancer immunomodulation using epithelial organoid cultures
Yotam E. Bar-Ephraim, Kai Kretzschmar, Priyanca Asra, Evelien de Jongh, Kim E. Boonekamp, Jarno Drost, Joost van Gorp, Apollo Pronk, Niels Smakman, Inez J. Gan, Zsolt Sebestyén, Jürgen Kuball, Robert G.J. Vries, Hans Clevers
bioRxiv 377655; doi: https://doi.org/10.1101/377655
Modelling cancer immunomodulation using epithelial organoid cultures
Yotam E. Bar-Ephraim, Kai Kretzschmar, Priyanca Asra, Evelien de Jongh, Kim E. Boonekamp, Jarno Drost, Joost van Gorp, Apollo Pronk, Niels Smakman, Inez J. Gan, Zsolt Sebestyén, Jürgen Kuball, Robert G.J. Vries, Hans Clevers
bioRxiv 377655; doi: https://doi.org/10.1101/377655
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