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Biotrophic interactions disentangled: In-situ localisation of mRNAs to decipher plant and algal pathogen – host interactions at single cell level

Julia Badstöber, Claire M. M. Gachon, Adolf M. Sandbichler, View ORCID ProfileSigrid Neuhauser
doi: https://doi.org/10.1101/378794
Julia Badstöber
Institute of Microbiology, University of Innsbruck, A-6020 Innsbruck, Austria
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Claire M. M. Gachon
The Scottish Association for Marine Science, Scottish Marine Institute, Oban PA37 1QA, UK
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Adolf M. Sandbichler
Institute of Zoology, University of Innsbruck, A-6020 Innsbruck, Austria
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Sigrid Neuhauser
Institute of Microbiology, University of Innsbruck, A-6020 Innsbruck, Austria
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  • ORCID record for Sigrid Neuhauser
  • For correspondence: Sigrid.Neuhauser@uibk.ac.at
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Summary

Plant-pathogen interactions follow spatial and temporal developmental dynamics where gene expression in pathogen and host undergo crucial changes. Therefore, it is of great interest to detect, quantify and localise where and when key genes are active to understand these processes. Many pathosystems are not accessible for genetic amendments or other spatially-resolved gene expression monitoring methods. Here, we adapt single molecule FISH techniques to demonstrate the presence and activity of mRNAs at the single-cell level using phytomyxids in their plant and algal host in lab and field material. This allowed us to monitor and quantify the expression of genes from the clubroot pathogen Plasmodiophora brassicae, several species of its Brassica hosts, and of several brown algae, including the genome model Ectocarpus siliculosus, infected with the phytomyxid Maullinia ectocarpii. We show that mRNAs are localised along a spatiotemporal gradient, thus providing a proof-of-concept of the usefulness of single-molecule FISH to increase knowledge about the interactions between plants, algae and phytomyxids. The methods used are easily applicable to any interaction between microbes and their algal or plant host, and have therefore the potential to rapidly increase our understanding of key, spatially-and temporally-resolved processes underpinning complex plant-microbe interactions.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted May 02, 2019.
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Biotrophic interactions disentangled: In-situ localisation of mRNAs to decipher plant and algal pathogen – host interactions at single cell level
Julia Badstöber, Claire M. M. Gachon, Adolf M. Sandbichler, Sigrid Neuhauser
bioRxiv 378794; doi: https://doi.org/10.1101/378794
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Biotrophic interactions disentangled: In-situ localisation of mRNAs to decipher plant and algal pathogen – host interactions at single cell level
Julia Badstöber, Claire M. M. Gachon, Adolf M. Sandbichler, Sigrid Neuhauser
bioRxiv 378794; doi: https://doi.org/10.1101/378794

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