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Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage

Daniel Clarke, Adi Idris, Nigel AJ McMillan
doi: https://doi.org/10.1101/390641
Daniel Clarke
Menzies Health Institute Queensland, School of Medical Science, Griffith University, Southport, Australia
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Adi Idris
Menzies Health Institute Queensland, School of Medical Science, Griffith University, Southport, Australia
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  • For correspondence: a.idris@griffith.edu.au
Nigel AJ McMillan
Menzies Health Institute Queensland, School of Medical Science, Griffith University, Southport, Australia
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ABSTRACT

Liposomes are versatile and well-proven as a means to deliver nucleic acids into cells. Most of the formulation procedures used are labour intensive and result in unstable end products. We have previously reported on the development of a simple, yet efficient, hydration-of-freeze-dried-matrix (HFDM) method to entrap siRNA within lipid particles. Here we show that the particles are stable up to 12 months after storage room temperature (RT), 4°C or - 20°C. While RT storage results in changes in particle size and polydispersity, gene silencing of all particles was similar to freshly prepared particles following storage for 3, 6, 9 or 12 months at all temperatures. This is the first report of such long-term stability in siRNA-loaded liposomes.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted August 13, 2018.
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Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage
Daniel Clarke, Adi Idris, Nigel AJ McMillan
bioRxiv 390641; doi: https://doi.org/10.1101/390641
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Development of novel lipidic particles for siRNA delivery that are highly effective after 12 months storage
Daniel Clarke, Adi Idris, Nigel AJ McMillan
bioRxiv 390641; doi: https://doi.org/10.1101/390641

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