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There and back again: metagenome-assembled genomes provide new insights into two thermal pools in Kamchatka, Russia

View ORCID ProfileLaetitia G. E. Wilkins, View ORCID ProfileCassandra L. Ettinger, View ORCID ProfileGuillaume Jospin, View ORCID ProfileJonathan A. Eisen
doi: https://doi.org/10.1101/392308
Laetitia G. E. Wilkins
1Department of Environmental Sciences, Policy &Management, University of California, Berkeley, CA USA
2Genome Center, University of California, Davis, CA USA
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  • For correspondence: laetitia.wilkins@berkeley.edu
Cassandra L. Ettinger
2Genome Center, University of California, Davis, CA USA
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Guillaume Jospin
2Genome Center, University of California, Davis, CA USA
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Jonathan A. Eisen
2Genome Center, University of California, Davis, CA USA
3Department of Evolution and Ecology, University of California, Davis, CA USA
4Department of Medical Microbiology and Immunology, University of California, Davis, CA USA
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Abstract

Culture-independent methods have contributed substantially to our understanding of global microbial diversity. Recently developed algorithms to construct whole genomes from environmental samples have further refined, corrected and revolutionized the tree of life. Here, we assembled draft metagenome-assembled genomes (MAGs) from environmental DNA extracted from two hot springs within an active volcanic ecosystem on the Kamchatka peninsula, Russia. This hydrothermal system has been intensively studied previously with regard to geochemistry, chemoautotrophy, microbial isolation, and microbial diversity. Using a shotgun metagenomics approach, we assembled population-level genomes of bacteria and archaea from two pools using DNA that had previously been characterized via 16S rRNA gene clone libraries. We recovered 36 MAGs, 29 of medium to high quality, and placed them in the context of the current microbial tree of life. We highlight MAGs representing previously underrepresented archaeal phyla (Korarchaeota, Bathyarchaeota and Aciduliprofundum) and one potentially new species within the bacterial genus Sulfurihydrogenibium. Putative functions in both pools were compared and are discussed in the context of their diverging geochemistry. This study can be considered complementary to foregoing studies in the same ecosystem as it adds more comprehensive information about phylogenetic diversity and functional potential within this highly selective habitat.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 15, 2018.
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There and back again: metagenome-assembled genomes provide new insights into two thermal pools in Kamchatka, Russia
Laetitia G. E. Wilkins, Cassandra L. Ettinger, Guillaume Jospin, Jonathan A. Eisen
bioRxiv 392308; doi: https://doi.org/10.1101/392308
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There and back again: metagenome-assembled genomes provide new insights into two thermal pools in Kamchatka, Russia
Laetitia G. E. Wilkins, Cassandra L. Ettinger, Guillaume Jospin, Jonathan A. Eisen
bioRxiv 392308; doi: https://doi.org/10.1101/392308

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