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Whole-neuron synaptic mapping reveals local balance between excitatory and inhibitory synapse organization

Daniel Maxim Iascone, Yujie Li, Uygar Sümbül, Michael Doron, Hanbo Chen, Valentine Andreu, Finola Goudy, Idan Segev, Hanchuan Peng, View ORCID ProfileFranck Polleux
doi: https://doi.org/10.1101/395384
Daniel Maxim Iascone
1Department of Neuroscience, Columbia University, New York, NY, USA
2Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA
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Yujie Li
3Allen Institute for Brain Science, Seattle, WA, USA
4Department of Computer Science, University of Georgia, Athens, GA, USA
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Uygar Sümbül
3Allen Institute for Brain Science, Seattle, WA, USA
5Department of Statistics, Columbia University, New York, NY, USA
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Michael Doron
6Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, Jerusalem, Israel.
7Department of Neurobiology, The Hebrew University, Jerusalem, Israel
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Hanbo Chen
3Allen Institute for Brain Science, Seattle, WA, USA
4Department of Computer Science, University of Georgia, Athens, GA, USA
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Valentine Andreu
8Department of Biology, Barnard College, New York, NY, USA
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Finola Goudy
8Department of Biology, Barnard College, New York, NY, USA
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Idan Segev
6Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, Jerusalem, Israel.
7Department of Neurobiology, The Hebrew University, Jerusalem, Israel
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Hanchuan Peng
3Allen Institute for Brain Science, Seattle, WA, USA
9Southeast University – Allen Institute Joint Center for Neuron Morphology, Southeast University, Nanjing, China
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Franck Polleux
1Department of Neuroscience, Columbia University, New York, NY, USA
2Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY, USA
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  • ORCID record for Franck Polleux
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SUMMARY

The balance between excitatory and inhibitory (E and I) synaptic inputs is thought to be critical for information processing in neural circuits. However, little is known about the principles of spatial organization of E and I synapses across the entire dendritic tree of mammalian neurons. We developed a new, open-source, reconstruction platform for mapping the size and spatial distribution of E and I synapses received by individual, genetically-labeled, layer 2/3 cortical pyramidal neurons (PNs) in vivo. We mapped over 90,000 E and I synapses across twelve L2/3 PNs and uncovered structured organization of E and I synapses across dendritic domains as well as within individual dendritic segments in these cells. Despite significant, domain-specific, variations in the absolute density of E and I synapses, their ratio is strikingly balanced locally across dendritic segments. Computational modeling indicates that this spatially-precise E/I balance dampens dendritic voltage fluctuations and strongly impacts neuronal firing output.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 19, 2018.
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Whole-neuron synaptic mapping reveals local balance between excitatory and inhibitory synapse organization
Daniel Maxim Iascone, Yujie Li, Uygar Sümbül, Michael Doron, Hanbo Chen, Valentine Andreu, Finola Goudy, Idan Segev, Hanchuan Peng, Franck Polleux
bioRxiv 395384; doi: https://doi.org/10.1101/395384
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Whole-neuron synaptic mapping reveals local balance between excitatory and inhibitory synapse organization
Daniel Maxim Iascone, Yujie Li, Uygar Sümbül, Michael Doron, Hanbo Chen, Valentine Andreu, Finola Goudy, Idan Segev, Hanchuan Peng, Franck Polleux
bioRxiv 395384; doi: https://doi.org/10.1101/395384

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