Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The fungal root endophyte Serendipita indica modifies extracellular nucleotides to subvert plant immunity

Shadab Nizam, Xiaoyu Qiang, Stephan Wawra, Robin Nostadt, Felix Getzke, Florian Schwanke, View ORCID ProfileIngo Dreyer, Gregor Langen, Alga Zuccaro
doi: https://doi.org/10.1101/396028
Shadab Nizam
1Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 10, 35043 Marburg, Germany
2University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, Zülpicher Str. 47b, 50674 Cologne, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xiaoyu Qiang
1Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 10, 35043 Marburg, Germany
2University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, Zülpicher Str. 47b, 50674 Cologne, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephan Wawra
2University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, Zülpicher Str. 47b, 50674 Cologne, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robin Nostadt
1Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 10, 35043 Marburg, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Felix Getzke
2University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, Zülpicher Str. 47b, 50674 Cologne, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Florian Schwanke
2University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, Zülpicher Str. 47b, 50674 Cologne, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ingo Dreyer
3Centro de Bioinformática y Simulación Molecular (CBSM), Universidad de Talca, Chile
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ingo Dreyer
Gregor Langen
2University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, Zülpicher Str. 47b, 50674 Cologne, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alga Zuccaro
1Max-Planck-Institute for Terrestrial Microbiology, Karl-von-Frisch Strasse 10, 35043 Marburg, Germany
2University of Cologne, Botanical Institute, Cluster of Excellence on Plant Sciences (CEPLAS), Cologne Biocenter, Zülpicher Str. 47b, 50674 Cologne, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

One sentence abstract Immune modulation by metabolites in plant fungus interaction

Abstract Extracellular adenosine 5′-triphosphate (eATP) is an essential signaling molecule that mediates different cellular processes through its interaction with membrane-associated receptor proteins in animals and plants. eATP regulates plant growth, development and responses to biotic and abiotic stresses. Its accumulation in the apoplast induces ROS production and cytoplasmic calcium increase mediating a defense response to invading microbes. We demonstrate that perception of eATP is important in plant-fungus interaction and that during colonization by the beneficial root endophyte Serendipita indica accumulation of eATP in the apoplast occurs at early symbiotic stages. We show by liquid chromatography-tandem mass spectrometry, cytological and functional analysis that S. indica subvert eATP host response by secreting SiE5’NT, an enzymatically active ecto-5′nucleotidase capable of hydrolyzing eATP to adenosine. A. thaliana lines producing extracellular SiE5’NT are signi?cantly better colonized and have reduced eATP levels and defense signaling, indicating that SiE5’NT functions as a compatibility factor. Our data show that extracellular bioactive nucleotides play an important role in fungus-root interactions and that fungi can modify plant derived metabolites in the apoplast to modulate host immunity.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted August 20, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
The fungal root endophyte Serendipita indica modifies extracellular nucleotides to subvert plant immunity
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
The fungal root endophyte Serendipita indica modifies extracellular nucleotides to subvert plant immunity
Shadab Nizam, Xiaoyu Qiang, Stephan Wawra, Robin Nostadt, Felix Getzke, Florian Schwanke, Ingo Dreyer, Gregor Langen, Alga Zuccaro
bioRxiv 396028; doi: https://doi.org/10.1101/396028
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
The fungal root endophyte Serendipita indica modifies extracellular nucleotides to subvert plant immunity
Shadab Nizam, Xiaoyu Qiang, Stephan Wawra, Robin Nostadt, Felix Getzke, Florian Schwanke, Ingo Dreyer, Gregor Langen, Alga Zuccaro
bioRxiv 396028; doi: https://doi.org/10.1101/396028

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Plant Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3514)
  • Biochemistry (7371)
  • Bioengineering (5347)
  • Bioinformatics (20329)
  • Biophysics (10048)
  • Cancer Biology (7782)
  • Cell Biology (11353)
  • Clinical Trials (138)
  • Developmental Biology (6454)
  • Ecology (9985)
  • Epidemiology (2065)
  • Evolutionary Biology (13361)
  • Genetics (9377)
  • Genomics (12616)
  • Immunology (7729)
  • Microbiology (19119)
  • Molecular Biology (7478)
  • Neuroscience (41163)
  • Paleontology (301)
  • Pathology (1235)
  • Pharmacology and Toxicology (2142)
  • Physiology (3183)
  • Plant Biology (6885)
  • Scientific Communication and Education (1276)
  • Synthetic Biology (1900)
  • Systems Biology (5329)
  • Zoology (1091)