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“Global Transcriptional Response to CRISPR/CAS9-AAV6 Based Genome Editing” Matches Transcriptional Response to Specific Small Molecule Perturbations

View ORCID ProfileAkpéli V. Nordor, View ORCID ProfileMartin J. Aryee, View ORCID ProfileGeoffrey H. Siwo
doi: https://doi.org/10.1101/399311
Akpéli V. Nordor
1Institut Curie, PSL Research University, Translational Research Department, Paris 75005, France
2Massachusetts General Hospital and Harvard Medical School, Department of Pathology and Center for Cancer Research, Charlestown, Massachusetts, MA 02129, USA
3Computational Epigenomics Group, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
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Martin J. Aryee
2Massachusetts General Hospital and Harvard Medical School, Department of Pathology and Center for Cancer Research, Charlestown, Massachusetts, MA 02129, USA
3Computational Epigenomics Group, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA
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Geoffrey H. Siwo
4Center for Research Computing, Eck Institute for Global Health, Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46656, USA
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Abstract

Cromer et al. [1] recently reported global transcriptional changes occuring in cells in response to CRISPR/Cas9 gene editing. Using a CD34+ hematopoietic and progenitor stem cell model, they observed differentially expressed genes enriched for immune, stress and apoptotic processes following treatment with a CRISPR/Cas9-AAV6 genome-editing system. Following treatment with Cas9’s mRNA they observed transcriptional changes enriched for viral response as well as a downregulation of metabolic and cell cycle processes. Similarly, they observed a downregulation of metabolic processes in response to electroporation. Surprisingly, no enrichment for viral response genes was observed following treatment with AAV6 while minor transcriptional changes enriched for DNA damage signature occurred in response to Cas9/sgRNA ribonucleoprotein.

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Posted August 24, 2018.
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“Global Transcriptional Response to CRISPR/CAS9-AAV6 Based Genome Editing” Matches Transcriptional Response to Specific Small Molecule Perturbations
Akpéli V. Nordor, Martin J. Aryee, Geoffrey H. Siwo
bioRxiv 399311; doi: https://doi.org/10.1101/399311
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“Global Transcriptional Response to CRISPR/CAS9-AAV6 Based Genome Editing” Matches Transcriptional Response to Specific Small Molecule Perturbations
Akpéli V. Nordor, Martin J. Aryee, Geoffrey H. Siwo
bioRxiv 399311; doi: https://doi.org/10.1101/399311

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