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Reduced insulin/IGF-1 signalling in adult parents increases offspring fitness

View ORCID ProfileMartin I. Lind, Sanjana Ravindran, Zuzana Sekajova, Hanne Carlsson, Andrea Hinas, Alexei A. Maklakov
doi: https://doi.org/10.1101/405019
Martin I. Lind
1Animal Ecology, Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, Uppsala 752 36, Sweden
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  • ORCID record for Martin I. Lind
  • For correspondence: martin.i.lind@gmail.com a.maklakov@uea.ac.uk
Sanjana Ravindran
1Animal Ecology, Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, Uppsala 752 36, Sweden
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Zuzana Sekajova
1Animal Ecology, Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, Uppsala 752 36, Sweden
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Hanne Carlsson
1Animal Ecology, Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, Uppsala 752 36, Sweden
2School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
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Andrea Hinas
3Department of Cell and Molecular Biology, Uppsala University, PO Box 596, Uppsala 751 24, Sweden
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Alexei A. Maklakov
1Animal Ecology, Department of Ecology and Genetics, Uppsala University, Norbyvägen 18D, Uppsala 752 36, Sweden
2School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
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  • For correspondence: martin.i.lind@gmail.com a.maklakov@uea.ac.uk
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Summary

Reduced expression of the insulin/insulin-like nutrient-sensing signalling (IIS) pathway gene daf-2 in adult Caenorhabditis elegans nematode worms increases longevity without affecting fecundity, but the effect of parental lifespan extension on adult offspring is largely unknown. Here we show that reduced IIS signalling in parental generation increases offspring fitness. We used RNA interference (RNAi) to silence daf-2 expression in sexually mature C. elegans hermaphrodites from three different genotypes: N2 wildtype, as well as ppw-1 and rrf-1 mutants that are deficient for RNAi in germline and soma, respectively. Long-lived daf-2 RNAi parents showed normal fecundity as self-fertilizing hermaphrodites and improved late-life reproduction when mated to males. Remarkably, the offspring of daf-2 RNAi parents produced more progeny and had higher Darwinian fitness across all three genotypes. Thus, reduced IIS signalling in adulthood improves offspring quality supporting the emerging view that suboptimally high levels of nutrient-sensing signalling in late-life lie at the heart of ageing.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted August 31, 2018.
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Reduced insulin/IGF-1 signalling in adult parents increases offspring fitness
Martin I. Lind, Sanjana Ravindran, Zuzana Sekajova, Hanne Carlsson, Andrea Hinas, Alexei A. Maklakov
bioRxiv 405019; doi: https://doi.org/10.1101/405019
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Reduced insulin/IGF-1 signalling in adult parents increases offspring fitness
Martin I. Lind, Sanjana Ravindran, Zuzana Sekajova, Hanne Carlsson, Andrea Hinas, Alexei A. Maklakov
bioRxiv 405019; doi: https://doi.org/10.1101/405019

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