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A SKI subcomplex specifically required for the degradation of ribosome-free RNA regions

Elodie Zhang, Varun Khanna, View ORCID ProfileAbdelkader Namane, Antonia Doyen, Estelle Dacheux, Bernard Turcotte, Alain Jacquier, View ORCID ProfileMicheline Fromont-Racine
doi: https://doi.org/10.1101/409490
Elodie Zhang
‡Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, UMR 3525, F-75724 Paris, France
1Sorbonne Universités, UPMC Paris 6, Complexité Du Vivant, 75252 Paris Cedex 05, France
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Varun Khanna
‡Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, UMR 3525, F-75724 Paris, France
2Hub Bioinformatique et Biostatistique, Institut Pasteur - C3BI, USR 3756 IP CNRS, Paris, 75015, France
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Abdelkader Namane
‡Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, UMR 3525, F-75724 Paris, France
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  • ORCID record for Abdelkader Namane
Antonia Doyen
‡Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, UMR 3525, F-75724 Paris, France
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Estelle Dacheux
‡Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, UMR 3525, F-75724 Paris, France
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Bernard Turcotte
§McGill University Health Centre, Department of Medicine, 1001 Décarie Boulevard, Montréal, Québec, Canada H4A 3J1
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Alain Jacquier
‡Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, UMR 3525, F-75724 Paris, France
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  • For correspondence: mfromont@pasteur.fr jacquier@pasteur.fr
Micheline Fromont-Racine
‡Institut Pasteur, Génétique des Interactions Macromoléculaires, Centre National de la Recherche Scientifique, UMR 3525, F-75724 Paris, France
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  • ORCID record for Micheline Fromont-Racine
  • For correspondence: mfromont@pasteur.fr jacquier@pasteur.fr
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Summary

The Ski2-Ski3-Ski8 (SKI) complex assists the RNA exosome during the 3’-5’ degradation of cytoplasmic transcripts. Previous reports showed that the SKI complex is involved in the 3’-5’ degradation of mRNA, including 3’ untranslated regions (UTRs), devoid of ribosomes. Paradoxically, we recently showed that the SKI complex directly interacts with ribosomes during the co-translational mRNA decay and that this interaction is necessary for its RNA degradation promoting activity. Here, we characterized a new SKI-associated factor, Ska1, which antagonizes the SKI-ribosome interaction. We showed that the SKI-Ska1-subcomplex is specifically involved in the degradation of ribosome-free RNA regions such as long mRNA 3’UTRs and cytoplasmic lncRNAs. We propose a model in which the SKI-exosome complex first targets ribosome-free RNA 3’ends in its Ska1-associated form. When the complex reaches the mRNA coding sequence, the Ska1-SKI-exosome complex is exchanged for the SKI-exosome, which interacts directly with ribosomes in order to resume the degradation process.

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Posted September 06, 2018.
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A SKI subcomplex specifically required for the degradation of ribosome-free RNA regions
Elodie Zhang, Varun Khanna, Abdelkader Namane, Antonia Doyen, Estelle Dacheux, Bernard Turcotte, Alain Jacquier, Micheline Fromont-Racine
bioRxiv 409490; doi: https://doi.org/10.1101/409490
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A SKI subcomplex specifically required for the degradation of ribosome-free RNA regions
Elodie Zhang, Varun Khanna, Abdelkader Namane, Antonia Doyen, Estelle Dacheux, Bernard Turcotte, Alain Jacquier, Micheline Fromont-Racine
bioRxiv 409490; doi: https://doi.org/10.1101/409490

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