Abstract
miR-155 is an important microRNA which has multiple functions in many physiological and pathological processes. In this study, partial pri-miR-155 sequences were cloned from AA+ broiler, Sanhuang broiler and Hi-Line Brown layer, respectively. Stem-loop RT-qPCR was performed to detect the miR-155 spatiotemporal expression profiles of each chicken breed. The results showed that the partial pri-miR-155 sequences of different breeds of chicken were high conserved. The expression patterns of miR-155 between broiler and layer were basically similar, and miR-155 is expressed highly in immune related tissues. Interestingly, miR-155 expression activity had higher level in fat tissue of the three chicken breeds (14-day-old), but it decreased significantly in fat of the Hi-Line Brown layer (10-month-old and 24-month-old). In addition, the expression activities of miR-155 in 14-day-old broilers (AA+ broiler and Sanhuang broiler) were significantly lower than that of Hi-Line Brown layer (14-day-old) (P<0.05). Moreover, miR-155 expression activities in skeletal muscle of 14-day-old and 10-month-old Hi-Line Brown layer were also significantly lower than that of 24-month-old layer (P<0.05). The results indicated that miR-155 might be one of the important factors affecting the differences in skeletal muscle development and adipogenesis between different chicken breeds. These data can serve as a foundation for further study the functions and mechanisms of miR-155 in the physiological and pathological contexts.
Abbreviations
- MEF2A
- Myogenic enhancer factor 2A
- BDNF
- Brain-derived neurotrophic factor
- OLFML3
- Olfactomedin-like 3
- Ogn
- osteoglycin
- C/EBPβ
- CCAAT/enhancer-binding protein β
- CREB
- cAMP-response element binding protein
- PPARγ
- peroxisome proliferator-activated receptor gamma
Abbreviations
- MEF2A
- Myogenic enhancer factor 2A
- BDNF
- Brain-derived neurotrophic factor
- OLFML3
- Olfactomedin-like 3
- Ogn
- osteoglycin
- C/EBPβ
- CCAAT/enhancer-binding protein β
- CREB
- cAMP-response element binding protein
- PPARγ
- peroxisome proliferator-activated receptor gamma