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FGF1 Promotes Xenopus laevis Lens Regeneration

Lisa Moore, View ORCID ProfileKimberly J. Perry, Cindy Sun, Jonathan J. Henry
doi: https://doi.org/10.1101/411991
Lisa Moore
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
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Kimberly J. Perry
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
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  • ORCID record for Kimberly J. Perry
Cindy Sun
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
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Jonathan J. Henry
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois
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  • For correspondence: j-henry4@illinois.edu
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Abstract

Background The frog Xenopus laevis has notable regenerative capabilities, including that of the lens. The neural retina provides the factors that trigger lens regeneration from the cornea, but the identity of these factors is largely unknown. In contrast to the cornea, fibroblast growth factors FGF1, 8, and 9 are highly expressed within the retina, and are potential candidates for those factors. The purpose of this study is to determine whether specific FGF proteins can induce lens formation, and if perturbation of FGFR signaling inhibits lens regeneration.

Methods A novel cornea epithelial culture method was developed to investigate the sufficiency of FGFs in lens regeneration. Additionally, transgenic larvae expressing dominant negative FGFR1 were used to investigate the necessity of FGFR signaling in lens regeneration.

Results Treatment of cultured corneas with FGF1 induced lens regeneration in a dose-dependent manner, whereas treatment with FGF2, FGF8, or FGF9 did not result in significant lens regeneration. Inhibition of FGFR signaling decreased the lens regeneration rate for in vitro eye cultures.

Conclusion The culture techniques developed here, and elsewhere, have provided reliable methods for examining the necessity of various factors that may be involved in lens regeneration. Based on the results demonstrated in this study, we found that FGF1 signaling and FGFR activation are key factors for lens regeneration in Xenopus.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 07, 2018.
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FGF1 Promotes Xenopus laevis Lens Regeneration
Lisa Moore, Kimberly J. Perry, Cindy Sun, Jonathan J. Henry
bioRxiv 411991; doi: https://doi.org/10.1101/411991
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FGF1 Promotes Xenopus laevis Lens Regeneration
Lisa Moore, Kimberly J. Perry, Cindy Sun, Jonathan J. Henry
bioRxiv 411991; doi: https://doi.org/10.1101/411991

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