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Cytoskeleton mechanics determine resting size and activation dynamics of platelets

Aastha Mathur, Sandra Raquel Correia, View ORCID ProfileSerge Dmitrieff, View ORCID ProfileRomain Gibeaux, Iana Kalinina, Tooba Quidwai, Jonas Ries, Francois Nedelec
doi: https://doi.org/10.1101/413377
Aastha Mathur
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Sandra Raquel Correia
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Serge Dmitrieff
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Romain Gibeaux
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Iana Kalinina
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Tooba Quidwai
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Jonas Ries
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Francois Nedelec
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Summary

Platelets are cell fragments of various size that help maintain hemostasis. The way platelets respond during a clotting process is known to depend on their size, with important physiological consequences. We characterized the cytoskeleton of platelets as a function of their size. In resting Human and Mice platelets, we find a quadradic law between the size of a platelet and the amount of microtubule polymer it contains. We further estimate the length and number of microtubules in the marginal band using Electron and Super-resolution microscopy. In platelets activated with ADP, the marginal band coils as a consequence of cortical contraction driven by actin. We observe that this elastic coiling response is accompanied by a reversible shortening of the marginal band. Moreover, larger platelets have a higher propensity to coil. These results establish the dynamic equilibrium that is responsible for platelet size and differential response on a more quantitative level.

Highlights

  • Platelet size scales consistently with amount of polymerized tubulin in both mouse and human.

  • Polymerized actin is required for ADP-induced marginal band coiling.

  • Upon activation, the marginal band exhibits a reversible visco-elastic response involving shortening.

  • Larger marginal bands have a higher propensity to coil than shorter ones.

In brief The cytoskeleton is adapted to platelet size and its mechanical properties determine propensity of a platelet to undergo morphological changes in response to agonists.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted September 10, 2018.
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Cytoskeleton mechanics determine resting size and activation dynamics of platelets
Aastha Mathur, Sandra Raquel Correia, Serge Dmitrieff, Romain Gibeaux, Iana Kalinina, Tooba Quidwai, Jonas Ries, Francois Nedelec
bioRxiv 413377; doi: https://doi.org/10.1101/413377
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Cytoskeleton mechanics determine resting size and activation dynamics of platelets
Aastha Mathur, Sandra Raquel Correia, Serge Dmitrieff, Romain Gibeaux, Iana Kalinina, Tooba Quidwai, Jonas Ries, Francois Nedelec
bioRxiv 413377; doi: https://doi.org/10.1101/413377

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