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Cex1 is a new component of the COPI Golgi-to-vacuole intracellular trafficking machinery

View ORCID ProfileLudovic Enkler, Johann Owen de Craene, Bruno Rinaldi, Philippe Hammann, Osamu Nureki, Bruno Senger, Sylvie Friant, Hubert D. Becker
doi: https://doi.org/10.1101/414367
Ludovic Enkler
1UMR 7156 ’Génétique Moléculaire, Génomique, Microbiologie’, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France.
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  • ORCID record for Ludovic Enkler
  • For correspondence: h.becker@unistra.fr enkler@unistra.fr
Johann Owen de Craene
1UMR 7156 ’Génétique Moléculaire, Génomique, Microbiologie’, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France.
2UPR ’Architecture et Réactivité de l’ARN’, Université de Strasbourg, CNRS, Institut de Biologie Moléculaire et Cellulaire, 15, Rue René Descartes, F-67084 Strasbourg Cedex, France.
3Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032.
4EA 2106, Biomolécules et Biotechnologies Végétales, Université François Rabelais de Tours, UFR Sciences et Techniques, Parc de Grandmont 37200 Tours, France.
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Bruno Rinaldi
1UMR 7156 ’Génétique Moléculaire, Génomique, Microbiologie’, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France.
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Philippe Hammann
2UPR ’Architecture et Réactivité de l’ARN’, Université de Strasbourg, CNRS, Institut de Biologie Moléculaire et Cellulaire, 15, Rue René Descartes, F-67084 Strasbourg Cedex, France.
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Osamu Nureki
3Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-0032.
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Bruno Senger
1UMR 7156 ’Génétique Moléculaire, Génomique, Microbiologie’, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France.
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Sylvie Friant
1UMR 7156 ’Génétique Moléculaire, Génomique, Microbiologie’, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France.
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Hubert D. Becker
1UMR 7156 ’Génétique Moléculaire, Génomique, Microbiologie’, CNRS, Université de Strasbourg, 21 Rue René Descartes, 67084 Strasbourg Cedex, France.
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  • For correspondence: h.becker@unistra.fr enkler@unistra.fr
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Abstract

During translational elongation, aminoacylated tRNA are supplied to the ribosome by RNA-interacting proteins. Cex1, a HEAT-containing protein, has been shown to participate to this tRNA channeling by interacting with aminoacylated tRNA during their export from the nucleus. Here, we show that Cex1 is a component of COPI (coatomer complex I) coated vesicles involved in the Golgi-to-vacuole trafficking pathway in Saccharomyces cerevisiae. Cex1 interacts with key components of the COPI coat Sec27, Sec28 and Sec33 proteins. Moreover, fluorescent microscopy indicates that Cex1 is not localized at the nuclear periphery as expected for an effector of nuclear tRNA channeling, but is observed on endosomal trans-Golgi network (TGN) positive structures. This localization relies on the vacuolar protein sorting receptor Vps10. Our data not only resolve the functional ambiguity regarding Cex1 homologues across species, but also point to the possibility to develop yeast-based models to study neurodegenerative disorders linked to the Human Cex1 homologue SCYL1.

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Posted September 20, 2018.
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Cex1 is a new component of the COPI Golgi-to-vacuole intracellular trafficking machinery
Ludovic Enkler, Johann Owen de Craene, Bruno Rinaldi, Philippe Hammann, Osamu Nureki, Bruno Senger, Sylvie Friant, Hubert D. Becker
bioRxiv 414367; doi: https://doi.org/10.1101/414367
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Cex1 is a new component of the COPI Golgi-to-vacuole intracellular trafficking machinery
Ludovic Enkler, Johann Owen de Craene, Bruno Rinaldi, Philippe Hammann, Osamu Nureki, Bruno Senger, Sylvie Friant, Hubert D. Becker
bioRxiv 414367; doi: https://doi.org/10.1101/414367

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