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Synthetic Metabolic Pathway for the Production of 1-Alkenes from Lignin-derived Molecules

Jin Luo, Tapio Lehtinen, Elena Efimova, Ville Santala, Suvi Santala
doi: https://doi.org/10.1101/417857
Jin Luo
Department of Chemistry and Bioengineering, Tampere University of Technology, 33720, Tampere, Finland
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  • For correspondence: jin.luo@tut.fi
Tapio Lehtinen
Department of Chemistry and Bioengineering, Tampere University of Technology, 33720, Tampere, Finland
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Elena Efimova
Department of Chemistry and Bioengineering, Tampere University of Technology, 33720, Tampere, Finland
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Ville Santala
Department of Chemistry and Bioengineering, Tampere University of Technology, 33720, Tampere, Finland
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Suvi Santala
Department of Chemistry and Bioengineering, Tampere University of Technology, 33720, Tampere, Finland
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Abstract

Integration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use of abundant waste material to produce readily collectable product, minimizing production costs. Towards that end, we established the production of semivolatile medium-chain α-olefins from lignin-derived monomers: we constructed 1-undecene synthesis pathway in Acinetobacter baylyi ADP1 using ferulate as the sole carbon source. In order to overcome the toxicity of ferulate, we first applied adaptive laboratory evolution, resulting in a highly ferulate-tolerant strain. Next, we demonstrated the 1-undecene production from glucose by heterologously expressing a fatty acid decarboxylase UndA and a thioesterase ‘TesA in the wild type strain. Finally, we constructed the alkene synthesis pathway in the ferulate-tolerant strain. We were able to produce 1-undecene from ferulate and collect the product from the culture headspace without downstream processing. This study demonstrates the potential of bacterial lignin upgradation into value-added products.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted September 14, 2018.
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Synthetic Metabolic Pathway for the Production of 1-Alkenes from Lignin-derived Molecules
Jin Luo, Tapio Lehtinen, Elena Efimova, Ville Santala, Suvi Santala
bioRxiv 417857; doi: https://doi.org/10.1101/417857
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Synthetic Metabolic Pathway for the Production of 1-Alkenes from Lignin-derived Molecules
Jin Luo, Tapio Lehtinen, Elena Efimova, Ville Santala, Suvi Santala
bioRxiv 417857; doi: https://doi.org/10.1101/417857

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