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Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly

Adam R. Lamson, Christopher Edelmaier, Matthew A. Glaser, View ORCID ProfileMeredith D. Betterton
doi: https://doi.org/10.1101/419135
Adam R. Lamson
Department of Physics, University of Colorado, Boulder, CO 80309, USA
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Christopher Edelmaier
Department of Physics, University of Colorado, Boulder, CO 80309, USA
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Matthew A. Glaser
Department of Physics, University of Colorado, Boulder, CO 80309, USA
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Meredith D. Betterton
Department of Physics, University of Colorado, Boulder, CO 80309, USA
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  • ORCID record for Meredith D. Betterton
  • For correspondence: mdb@colorado.edu
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Abstract

Cells grow, move, and respond to outside stimuli by large-scale cytoskeletal reorganization. A prototypical example of cytoskeletal remodeling is mitotic spindle assembly, where microtubules nucleate, undergo dynamic instability, bundle, and organize into a bipolar spindle. Key mechanisms of this process include regulated filament polymerization, crosslinking, and motor-protein activity. Remarkably, fission yeast can assemble a bipolar spindle using only passive crosslinkers. We develop a torque-balance model that describes this reorganization due to dynamic microtubule bundles, spindle-pole bodies, the nuclear envelope, and crosslinkers to predict spindle-assembly dynamics. We compare these results to those obtained with kinetic Monte Carlo-Brownian dynamics simulations, which introduce crosslinker-binding kinetics and other stochastic effects. Our results show that rapid crosslinker reorganization to MT overlaps facilitates crosslinker-driven spindle assembly, a testable prediction for future experiments. Combining these two modeling techniques, we illustrate a general method for studying cytoskeletal network reorganization.

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Posted September 22, 2018.
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Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly
Adam R. Lamson, Christopher Edelmaier, Matthew A. Glaser, Meredith D. Betterton
bioRxiv 419135; doi: https://doi.org/10.1101/419135
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Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly
Adam R. Lamson, Christopher Edelmaier, Matthew A. Glaser, Meredith D. Betterton
bioRxiv 419135; doi: https://doi.org/10.1101/419135

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