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Tracking transient fluorescent events in structured point clouds

View ORCID ProfileSaoirse Amarteifio, Todd Fallesen, Giovanni Sena, Gunnar Pruessner
doi: https://doi.org/10.1101/419572
Saoirse Amarteifio
Department of Mathematics, Imperial College London
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Todd Fallesen
Department of Life Sciences, Imperial College London
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Giovanni Sena
Department of Life Sciences, Imperial College London
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Gunnar Pruessner
Department of Mathematics, Imperial College London
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Abstract

Tracking algorithms used in conjunction with fluorescent time-lapse microscopy data typically assume a continuous signal where (a) background and keypoints are permanently visible and (b) independently moving objects of interest are permanently visible when not occluded. These conditions allow for registration and identity management algorithms to track independently-moving objects of interest over time. In contrast to such conditions, we consider the case of (a) transient fluorescent events and (b) objects embedded in a possibly imperceptible, almost rigid structure, which acts to constrain independent object movement. In a biological context, such events could for example represent cell divisions in a growing tissue. Such conditions motivate the merging of registration and tracking tasks into a fuzzy registration algorithm to solve the identity management problem. We describe the design and application of such an algorithm, illustrated in the domain of plant biology and made available as an open source software implementation.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted September 18, 2018.
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Tracking transient fluorescent events in structured point clouds
Saoirse Amarteifio, Todd Fallesen, Giovanni Sena, Gunnar Pruessner
bioRxiv 419572; doi: https://doi.org/10.1101/419572
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Tracking transient fluorescent events in structured point clouds
Saoirse Amarteifio, Todd Fallesen, Giovanni Sena, Gunnar Pruessner
bioRxiv 419572; doi: https://doi.org/10.1101/419572

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