Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

A specific Ret receptor isoform is required for pioneer axon outgrowth and growth cone dynamics

Adam M. Tuttle, Catherine M. Drerup, Molly H. Marra, Alex V. Nechiporuk
doi: https://doi.org/10.1101/424432
Adam M. Tuttle
1Department of Cell, Developmental, & Cancer Biology, Oregon Health & Science University, Portland, OR 97239, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Catherine M. Drerup
1Department of Cell, Developmental, & Cancer Biology, Oregon Health & Science University, Portland, OR 97239, USA
2Unit on Neuronal Cell Biology, National Institute of Child Health & Human Development, National Institutes of Health, Bethesda, MD 20892, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Molly H. Marra
1Department of Cell, Developmental, & Cancer Biology, Oregon Health & Science University, Portland, OR 97239, USA
3Neuroscience Graduate Program, Oregon Health & Science University, Portland, OR 97239, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alex V. Nechiporuk
1Department of Cell, Developmental, & Cancer Biology, Oregon Health & Science University, Portland, OR 97239, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: nechipor@ohsu.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

In many cases, axon growth and guidance are driven by pioneer axons, the first axons to grow in a particular region. Despite their dynamic pathfinding capabilities and developmental importance, there are very few pioneer neuron specific markers and thus their in vivo identification and functional interrogation have been difficult. We found that a Ret receptor isoform, Ret51, is highly enriched in peripheral sensory pioneer neurons and is required for pioneer axon outgrowth. Ret null mutant pioneer neurons differentiate normally; however, they displayed defects in growth cone morphology and formation of filopodia before pioneer axon extension prematurely halts. We also demonstrate loss-of-function of a retrograde cargo adaptor, JNK-interacting protein 3 (Jip3), phenocopied many of these axonal defects. We further found that loss of Jip3 led to accumulation of activated Ret receptor in pioneer growth cones, indicating a failure in the clearance of activated Ret from growth cones. Using an axon sever approach as well as in vivo analysis of axonal transport, we showed Jip3 specifically mediates retrograde, but not anterograde, transport of activated Ret51. Finally, live imaging revealed that Jip3 and Ret51 were retrogradely co-transported in pioneer axons, suggesting Jip3 functions as an adapter for retrograde transport of Ret51. Taken together, these results identify Ret51 as a molecular marker of pioneer neurons and elucidate an important isoform-specific role for Ret51 in axon growth and growth cone dynamics during development.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted September 21, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
A specific Ret receptor isoform is required for pioneer axon outgrowth and growth cone dynamics
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
A specific Ret receptor isoform is required for pioneer axon outgrowth and growth cone dynamics
Adam M. Tuttle, Catherine M. Drerup, Molly H. Marra, Alex V. Nechiporuk
bioRxiv 424432; doi: https://doi.org/10.1101/424432
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
A specific Ret receptor isoform is required for pioneer axon outgrowth and growth cone dynamics
Adam M. Tuttle, Catherine M. Drerup, Molly H. Marra, Alex V. Nechiporuk
bioRxiv 424432; doi: https://doi.org/10.1101/424432

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Developmental Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2517)
  • Biochemistry (4964)
  • Bioengineering (3469)
  • Bioinformatics (15181)
  • Biophysics (6885)
  • Cancer Biology (5380)
  • Cell Biology (7711)
  • Clinical Trials (138)
  • Developmental Biology (4518)
  • Ecology (7135)
  • Epidemiology (2059)
  • Evolutionary Biology (10210)
  • Genetics (7497)
  • Genomics (9767)
  • Immunology (4822)
  • Microbiology (13179)
  • Molecular Biology (5129)
  • Neuroscience (29367)
  • Paleontology (203)
  • Pathology (835)
  • Pharmacology and Toxicology (1460)
  • Physiology (2129)
  • Plant Biology (4734)
  • Scientific Communication and Education (1008)
  • Synthetic Biology (1337)
  • Systems Biology (4002)
  • Zoology (768)