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Mesoscale cortical calcium imaging reveals widespread synchronized infraslow activity during social touch in mice

View ORCID ProfileFederico Bolaños, Luis A. Bolaños, Matilde Balbi, Nicholas J. Michelson, Jeffrey M. LeDue, Timothy H. Murphy
doi: https://doi.org/10.1101/430306
Federico Bolaños
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research;
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
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  • ORCID record for Federico Bolaños
Luis A. Bolaños
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research;
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
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Matilde Balbi
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research;
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
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Nicholas J. Michelson
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research;
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
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Jeffrey M. LeDue
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research;
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
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Timothy H. Murphy
1Department of Psychiatry, Kinsmen Laboratory of Neurological Research;
2Djavad Mowafaghian Centre for Brain Health, University of British Columbia, Vancouver, British Columbia, Canada, V6T 1Z3
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Abstract

We employ cortical mesoscale GCaMP6s imaging of intracellular calcium levels to establish how brain activity is correlated when two mice engage in a staged social touch-like interaction. Using a rail system, two head-fixed mice begin at a distance where social touch is not possible (160 mm), after 90s they are brought so that macrovibrissae contact each other (6-12 mm snout to snout) for an additional 135s. During the period before, during, and after contact cortical mesoscale GCAMP6 signals were recorded from both mice simultaneously. When the mice were together we observed bouts of mutual whisking resulting in cross-mouse correlated barrel cortex activity. While correlations between whisker cortices were expected given mutual whisking, we also found significant synchronized brain-wide calcium signals at a frequency band of 0.01-0.1Hz when the mice were together. We present dual mouse brain imaging as new paradigm to assess social interactions in a more constrained manner. The effects of social interaction extend outside of regions associated with mutual touch and have global synchronizing effects on cortical activity.

Acknowledgements

This work was supported by a Canadian Institutes of Health Research (CIHR) T.H.M FDN-143209 and from Brain Canada for the Canadian Neurophotonics Platform to THM and the Brain Canada Multi-Investigator Research Initiative program that THM was part of. CIHR or Brain Canada had no involvement in the research or decision to publish. We thank Pumin Wang for help with surgery and Matthieu P. Vanni, Allen W. Chan, Dongsheng Xiao and Alexander McGirr for helpful discussion and comments.

Contributions

F.B., L.B., M.B., N.J.M., and T.H.M. performed animal experiments. F.B., T.H.M., and J.M.L.wrote the paper. F.B., L.B., J.M.L., and T.H.M. developed the hardware and software for the apparatus. F.B. and J.M.L wrote the analysis. L.B., F.B., and J.M.L drew models and figures.

Footnotes

  • Address: 2255 Wesbrook Mall, Detwiller Pavilion, Vancouver, B.C. V6T 1Z3, Canada, E-mail: thmurphy{at}mail.ubc.ca, federico.bolanos{at}riken.jp

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted October 04, 2018.
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Mesoscale cortical calcium imaging reveals widespread synchronized infraslow activity during social touch in mice
Federico Bolaños, Luis A. Bolaños, Matilde Balbi, Nicholas J. Michelson, Jeffrey M. LeDue, Timothy H. Murphy
bioRxiv 430306; doi: https://doi.org/10.1101/430306
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Mesoscale cortical calcium imaging reveals widespread synchronized infraslow activity during social touch in mice
Federico Bolaños, Luis A. Bolaños, Matilde Balbi, Nicholas J. Michelson, Jeffrey M. LeDue, Timothy H. Murphy
bioRxiv 430306; doi: https://doi.org/10.1101/430306

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