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Enhancing biochemical resolution by hyper-dimensional imaging microscopy

View ORCID ProfileAlessandro Esposito, Ashok R. Venkitaraman
doi: https://doi.org/10.1101/431452
Alessandro Esposito
The Medical Research Council Cancer Unit, University of Cambridge, Hills Road, Cambridge CB2 0XZ, UK
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Ashok R. Venkitaraman
The Medical Research Council Cancer Unit, University of Cambridge, Hills Road, Cambridge CB2 0XZ, UK
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Abstract

Two decades of high-paced innovation have improved the spatial resolution of fluorescence microscopy to enable molecular resolution combined with the low-invasiveness and specificity characteristic of optical microscopy. However, fluorescence microscopy also enables scientists and clinicians to map and quantitate the physico-chemical properties (e.g., analyte concentration, enzymatic activities and protein-protein interactions) of biological samples. But the optimization of the biochemical resolving power in fluorescence microscopy is not as well-developed compared to its spatial resolution. Typical techniques rely on the observation of individual properties of fluorescence thus limiting the opportunities for sensing and multiplexing. Aiming to overcome existing limitations, we demonstrate a new imaging paradigm — Hyper Dimensional Imaging Microscopy (HDIM) — that enables the orthogonal properties of fluorescence emission (excited state lifetime, polarization and spectra) in biological samples to be quantified simultaneously and efficiently. Therefore, akin to how multi-dimensional separation in mass-spectroscopy and multi-dimensional spectra in NMR impacted proteomics and structural biology, we envisage that HDIM spectra of unprecedented dimensionality will impact the fields of systems biology and medical diagnostics by maximizing the biochemical resolving power of fluorescence microscopy.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted October 02, 2018.
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Enhancing biochemical resolution by hyper-dimensional imaging microscopy
Alessandro Esposito, Ashok R. Venkitaraman
bioRxiv 431452; doi: https://doi.org/10.1101/431452
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Enhancing biochemical resolution by hyper-dimensional imaging microscopy
Alessandro Esposito, Ashok R. Venkitaraman
bioRxiv 431452; doi: https://doi.org/10.1101/431452

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