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Stage-Dependent Differential Gene Expression Profiles of Cranial Neural Crest Cells Derived from Mouse Induced Pluripotent Stem Cells

View ORCID ProfileAyano Odashima, Shoko Onodera, Akiko Saito, Takashi Nakamura, Yuuki Ogihara, Tatsuya Ichinohe, Toshifumi Azuma
doi: https://doi.org/10.1101/432302
Ayano Odashima
1Department of Oral Health Science Center, Tokyo Dental College, Tokyo, Japan
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Shoko Onodera
2Department of Biochemistry, Tokyo Dental College, Tokyo, Japan
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Akiko Saito
2Department of Biochemistry, Tokyo Dental College, Tokyo, Japan
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Takashi Nakamura
2Department of Biochemistry, Tokyo Dental College, Tokyo, Japan
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Yuuki Ogihara
3Department of Dental Anesthesiology, Tokyo Dental College, Tokyo, Japan
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Tatsuya Ichinohe
3Department of Dental Anesthesiology, Tokyo Dental College, Tokyo, Japan
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Toshifumi Azuma
1Department of Oral Health Science Center, Tokyo Dental College, Tokyo, Japan
2Department of Biochemistry, Tokyo Dental College, Tokyo, Japan
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  • For correspondence: satouayano@tdc.ac.jp
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Abstract

Cranial neural crest cells (cNCCs) comprise a multipotent population of cells that migrate into the pharyngeal arches of the vertebrate embryo and differentiate into a broad range of derivatives of the craniofacial organs. Consequently, migrating cNCCs are considered as one of the most attractive candidate sources of cells for regenerative medicine. In this study, we analyzed the gene expression profiles of cNCCs at different time points after induction by conducting three independent RNA sequencing experiments. We successfully induced cNCC formation from mouse induced pluripotent stem (miPS) cells by culturing them in neural crest inducing media for 14 days. We found that these cNCCs expressed several neural crest specifier genes but were lacking some previously reported specifiers, such as paired box 3 (Pax3), msh homeobox 1 (Msx1), and Forkhead box D3 (FoxD3), which are presumed to be essential for neural crest development in the embryo. Thus, a distinct molecular network may the control gene expression in miPS-derived cNCCs. We also found that c-Myc, ETS proto-oncogene 1, transcription factor (Ets1), and sex determining region Y-box 10 (Sox10) were only detected at 14 days after induction. Therefore, we assume that these genes would be useful markers for migratory cNCCs induced from miPS cells. Eventually, these cNCCs comprised a broad spectrum of protocadherin (Pcdh) and a disintegrin and metalloproteinase with thrombospondin motifs (Adamts) family proteins, which may be crucial in their migration.

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Posted October 01, 2018.
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Stage-Dependent Differential Gene Expression Profiles of Cranial Neural Crest Cells Derived from Mouse Induced Pluripotent Stem Cells
Ayano Odashima, Shoko Onodera, Akiko Saito, Takashi Nakamura, Yuuki Ogihara, Tatsuya Ichinohe, Toshifumi Azuma
bioRxiv 432302; doi: https://doi.org/10.1101/432302
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Stage-Dependent Differential Gene Expression Profiles of Cranial Neural Crest Cells Derived from Mouse Induced Pluripotent Stem Cells
Ayano Odashima, Shoko Onodera, Akiko Saito, Takashi Nakamura, Yuuki Ogihara, Tatsuya Ichinohe, Toshifumi Azuma
bioRxiv 432302; doi: https://doi.org/10.1101/432302

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