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Structural and proteomic changes in viable but non-culturable Vibrio cholerae

View ORCID ProfileSusanne Brenzinger, Lizah T. van der Aart, Gilles P. van Wezel, Jean-Marie Lacroix, Timo Glatter, View ORCID ProfileAriane Briegel
doi: https://doi.org/10.1101/433326
Susanne Brenzinger
1Institute of Biology, Leiden University of Leiden, 2333 BE Leiden, The Netherlands.
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Lizah T. van der Aart
1Institute of Biology, Leiden University of Leiden, 2333 BE Leiden, The Netherlands.
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Gilles P. van Wezel
1Institute of Biology, Leiden University of Leiden, 2333 BE Leiden, The Netherlands.
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Jean-Marie Lacroix
2Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, Université de Lille Sciences et Technologies, F-59655 Villeneuve d’Ascq, France.
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Timo Glatter
3Facility for bacterial Proteomics and Mass Spectrometry, Max-Planck Institute for terrestrial Microbiology, 35091 Marburg, Germany
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Ariane Briegel
1Institute of Biology, Leiden University of Leiden, 2333 BE Leiden, The Netherlands.
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Abstract

Aquatic environments are reservoirs of the human pathogen Vibrio cholerae O1, which causes the acute diarrheal disease cholera. Upon low temperature or limited nutrient availability, the cells enter a viable but non-culturable (VBNC) state. Characteristic of this state are an altered morphology, low metabolic activity and lack of growth under standard laboratory conditions. Here, for the first time, the cellular ultrastructure of V. cholerae VBNC cells raised in natural waters was investigated using electron cryo-tomography complemented by comparison of the proteomes and the peptidoglycan composition of LB overnight culture and VBNC cells. The extensive remodeling of the VBNC cells was most obvious in the passive dehiscence of the cell envelope, resulting in improper embedment of flagella and pili. Only minor changes of the peptidoglycan and osmoregulated periplasmic glucans were observed. Active changes in VBNC cells included the production of cluster I chemosensory arrays and change of abundance of cluster II array proteins. Components involved in iron acquisition and storage, peptide import and arginine biosynthesis were overrepresented in VBNC cells, while enzymes of the central carbon metabolism were found at lower levels. Finally, several pathogenicity factors of V. cholerae were less abundant in the VBNC state, potentially limiting their infectious potential.

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  • Competing Interests statement The authors declare that they have no conflict of interest.

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Posted October 02, 2018.
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Structural and proteomic changes in viable but non-culturable Vibrio cholerae
Susanne Brenzinger, Lizah T. van der Aart, Gilles P. van Wezel, Jean-Marie Lacroix, Timo Glatter, Ariane Briegel
bioRxiv 433326; doi: https://doi.org/10.1101/433326
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Structural and proteomic changes in viable but non-culturable Vibrio cholerae
Susanne Brenzinger, Lizah T. van der Aart, Gilles P. van Wezel, Jean-Marie Lacroix, Timo Glatter, Ariane Briegel
bioRxiv 433326; doi: https://doi.org/10.1101/433326

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