Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Wide-area all-optical neurophysiology in acute brain slices

Samouil L. Farhi, Vicente J. Parot, Abhinav Grama, Masahito Yamagata, Ahmed S. Abdelfattah, Yoav Adam, Shan Lou, Jeong Jun Kim, Robert E. Campbell, David D. Cox, Adam E. Cohen
doi: https://doi.org/10.1101/433953
Samouil L. Farhi
1Chemical Biology Program, Harvard University, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Vicente J. Parot
2Biophysics Program, Harvard University, Cambridge, Massachusetts, USA;
3Division of Health Science and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Abhinav Grama
4Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA;
5Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masahito Yamagata
4Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA;
5Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ahmed S. Abdelfattah
6Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yoav Adam
8Department of Chemistry and Chemical Biology Harvard University, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shan Lou
8Department of Chemistry and Chemical Biology Harvard University, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jeong Jun Kim
8Department of Chemistry and Chemical Biology Harvard University, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert E. Campbell
6Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David D. Cox
4Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA;
5Center for Brain Science, Harvard University, Cambridge, Massachusetts, USA;
7J.A. Paulson School of Engineering and Applied Sciences;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adam E. Cohen
8Department of Chemistry and Chemical Biology Harvard University, Cambridge, Massachusetts, USA;
9Department of Physics, Harvard University, Cambridge, Massachusetts, USA;
10Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts, USA;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: cohen@chemistry.harvard.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Optical tools for simultaneous perturbation and measurement of neural activity open the possibility of mapping neural function over wide areas of brain tissue. However, spectral overlap of actuators and reporters presents a challenge for their simultaneous use, and optical scattering and out-of-focus fluorescence in tissue degrade resolution. To minimize optical crosstalk, we combined an optimized variant (eTsChR) of the most blue-shifted channelrhodopsin reported to-date with a nuclear-localized red-shifted Ca2+ indicator, H2B-jRGECO1a. To perform wide-area optically sectioned imaging in tissue, we designed a structured illumination technique that uses Hadamard matrices to encode spatial information. By combining these molecular and optical approaches we made wide-area maps, spanning cortex and striatum, of the effects of antiepileptic drugs on neural excitability and on the effects of AMPA and NMDA receptor blockers on functional connectivity. Together, these tools provide a powerful capability for wide-area mapping of neuronal excitability and functional connectivity in acute brain slices.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted October 11, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Wide-area all-optical neurophysiology in acute brain slices
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Wide-area all-optical neurophysiology in acute brain slices
Samouil L. Farhi, Vicente J. Parot, Abhinav Grama, Masahito Yamagata, Ahmed S. Abdelfattah, Yoav Adam, Shan Lou, Jeong Jun Kim, Robert E. Campbell, David D. Cox, Adam E. Cohen
bioRxiv 433953; doi: https://doi.org/10.1101/433953
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Wide-area all-optical neurophysiology in acute brain slices
Samouil L. Farhi, Vicente J. Parot, Abhinav Grama, Masahito Yamagata, Ahmed S. Abdelfattah, Yoav Adam, Shan Lou, Jeong Jun Kim, Robert E. Campbell, David D. Cox, Adam E. Cohen
bioRxiv 433953; doi: https://doi.org/10.1101/433953

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (3609)
  • Biochemistry (7590)
  • Bioengineering (5533)
  • Bioinformatics (20833)
  • Biophysics (10347)
  • Cancer Biology (7998)
  • Cell Biology (11663)
  • Clinical Trials (138)
  • Developmental Biology (6619)
  • Ecology (10227)
  • Epidemiology (2065)
  • Evolutionary Biology (13648)
  • Genetics (9557)
  • Genomics (12860)
  • Immunology (7932)
  • Microbiology (19575)
  • Molecular Biology (7678)
  • Neuroscience (42193)
  • Paleontology (309)
  • Pathology (1259)
  • Pharmacology and Toxicology (2208)
  • Physiology (3272)
  • Plant Biology (7064)
  • Scientific Communication and Education (1295)
  • Synthetic Biology (1953)
  • Systems Biology (5435)
  • Zoology (1119)