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Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms

View ORCID ProfileAndres M Cardozo Gizzi, View ORCID ProfileDiego I. Cattoni, Jean-Bernard Fiche, Sergio Espinola, Julian Gurgo, Olivier Messina, Christophe Houbron, Yuki Ogiyama, Giorgio-Lucio Papadopoulos, Giacomo Cavalli, Mounia Lagha, Marcelo Nollmann
doi: https://doi.org/10.1101/434266
Andres M Cardozo Gizzi
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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  • ORCID record for Andres M Cardozo Gizzi
  • For correspondence: andresm.cardozo@gmail.com
Diego I. Cattoni
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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  • For correspondence: diecatto@gmail.com
Jean-Bernard Fiche
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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Sergio Espinola
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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Julian Gurgo
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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Olivier Messina
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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Christophe Houbron
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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Yuki Ogiyama
2 Institut de Génétique Humaine, CNRS UMR 9002, Université de Montpellier;
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Giorgio-Lucio Papadopoulos
2 Institut de Génétique Humaine, CNRS UMR 9002, Université de Montpellier;
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Giacomo Cavalli
2 Institut de Génétique Humaine, CNRS UMR 9002, Université de Montpellier;
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Mounia Lagha
3 Institut de Génétique Moleculaire de Montpellier, CNRS UMR 5535, Univ de Montpellier
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Marcelo Nollmann
1 Centre de Biochimie Structurale, CNRS UMR 5048, INSERM U1054, Université de Montpellier;
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  • For correspondence: marcelo.nollmann@cbs.cnrs.fr
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Abstract

Eukaryotic chromosomes are organized in multiple scales, from nucleosomes to chromosome territories. Recently, genome-wide methods identified an intermediate level of chromosome organization, topologically associating domains (TADs), that play key roles in transcriptional regulation. However, these methods cannot directly examine the interplay between transcriptional activation and chromosome architecture while maintaining spatial information. Here, we present a multiplexed, sequential imaging approach (Hi-M) that permits the simultaneous detection of chromosome organization and transcription in single nuclei. This allowed us to unveil the changes in 3D chromatin organization occurring upon transcriptional activation and homologous chromosome un-pairing during the awakening of the zygotic genome in intact Drosophila embryos. Excitingly, the ability of Hi-M to explore the multi-scale chromosome architecture with spatial resolution at different stages of development or during the cell cycle will be key to understand the mechanisms and consequences of the 4D organization of the genome.

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Posted January 21, 2019.
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Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms
Andres M Cardozo Gizzi, Diego I. Cattoni, Jean-Bernard Fiche, Sergio Espinola, Julian Gurgo, Olivier Messina, Christophe Houbron, Yuki Ogiyama, Giorgio-Lucio Papadopoulos, Giacomo Cavalli, Mounia Lagha, Marcelo Nollmann
bioRxiv 434266; doi: https://doi.org/10.1101/434266
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Microscopy-based chromosome conformation capture enables simultaneous visualization of genome organization and transcription in intact organisms
Andres M Cardozo Gizzi, Diego I. Cattoni, Jean-Bernard Fiche, Sergio Espinola, Julian Gurgo, Olivier Messina, Christophe Houbron, Yuki Ogiyama, Giorgio-Lucio Papadopoulos, Giacomo Cavalli, Mounia Lagha, Marcelo Nollmann
bioRxiv 434266; doi: https://doi.org/10.1101/434266

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