Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

Ahmed S. Abdelfattah, Takashi Kawashima, Amrita Singh, Ondrej Novak, Hui Liu, Yichun Shuai, Yi-Chieh Huang, Jonathan B. Grimm, Ronak Patel, Johannes Friedrich, Brett D. Mensh, Liam Paninski, John J. Macklin, Kaspar Podgorski, Bei-Jung Lin, Tsai-Wen Chen, Glenn C. Turner, Zhe Liu, Minoru Koyama, Karel Svoboda, Misha B. Ahrens, Luke D. Lavis, Eric R. Schreiter
doi: https://doi.org/10.1101/436840
Ahmed S. Abdelfattah
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Takashi Kawashima
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amrita Singh
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ondrej Novak
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hui Liu
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yichun Shuai
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yi-Chieh Huang
Institute of Neuroscience, National Yang-Ming University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan B. Grimm
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ronak Patel
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Johannes Friedrich
Columbia University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brett D. Mensh
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Liam Paninski
Columbia University
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John J. Macklin
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kaspar Podgorski
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bei-Jung Lin
Institute of Neuroscience, National Yang-Ming University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tsai-Wen Chen
Institute of Neuroscience, National Yang-Ming University;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Glenn C. Turner
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zhe Liu
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Minoru Koyama
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karel Svoboda
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Misha B. Ahrens
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Luke D. Lavis
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric R. Schreiter
Janelia Research Campus, Howard Hughes Medical Institute;
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: schreitere@janelia.hhmi.org
  • Abstract
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Imaging changes in membrane potential using genetically encoded fluorescent voltage indicators (GEVIs) has great potential for monitoring neuronal activity with high spatial and temporal resolution. Brightness and photostability of fluorescent proteins and rhodopsins have limited the utility of existing GEVIs. We engineered a novel GEVI, Voltron, that utilizes bright and photostable synthetic dyes instead of protein-based fluorophores, extending the combined duration of imaging and number of neurons imaged simultaneously by more than tenfold relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, zebrafish, and fruit flies. In mouse cortex, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously, over 15 minutes of continuous imaging. In larval zebrafish, Voltron enabled the precise correlation of spike timing with behavior.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted October 06, 2018.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
Share
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
Ahmed S. Abdelfattah, Takashi Kawashima, Amrita Singh, Ondrej Novak, Hui Liu, Yichun Shuai, Yi-Chieh Huang, Jonathan B. Grimm, Ronak Patel, Johannes Friedrich, Brett D. Mensh, Liam Paninski, John J. Macklin, Kaspar Podgorski, Bei-Jung Lin, Tsai-Wen Chen, Glenn C. Turner, Zhe Liu, Minoru Koyama, Karel Svoboda, Misha B. Ahrens, Luke D. Lavis, Eric R. Schreiter
bioRxiv 436840; doi: https://doi.org/10.1101/436840
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Bright and photostable chemigenetic indicators for extended in vivo voltage imaging
Ahmed S. Abdelfattah, Takashi Kawashima, Amrita Singh, Ondrej Novak, Hui Liu, Yichun Shuai, Yi-Chieh Huang, Jonathan B. Grimm, Ronak Patel, Johannes Friedrich, Brett D. Mensh, Liam Paninski, John J. Macklin, Kaspar Podgorski, Bei-Jung Lin, Tsai-Wen Chen, Glenn C. Turner, Zhe Liu, Minoru Koyama, Karel Svoboda, Misha B. Ahrens, Luke D. Lavis, Eric R. Schreiter
bioRxiv 436840; doi: https://doi.org/10.1101/436840

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (1003)
  • Biochemistry (1502)
  • Bioengineering (954)
  • Bioinformatics (6860)
  • Biophysics (2451)
  • Cancer Biology (1808)
  • Cell Biology (2547)
  • Clinical Trials (108)
  • Developmental Biology (1710)
  • Ecology (2589)
  • Epidemiology (1508)
  • Evolutionary Biology (5048)
  • Genetics (3632)
  • Genomics (4651)
  • Immunology (1186)
  • Microbiology (4275)
  • Molecular Biology (1637)
  • Neuroscience (10865)
  • Paleontology (83)
  • Pathology (243)
  • Pharmacology and Toxicology (411)
  • Physiology (560)
  • Plant Biology (1469)
  • Scientific Communication and Education (414)
  • Synthetic Biology (546)
  • Systems Biology (1891)
  • Zoology (261)