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Reconstitution of the equilibrium state of dynamic actin networks

Angelika Manhart, Aleksandra Icheva, Christophe Guerin, Tobbias Klar, Rajaa Boujemaa-Paterski, View ORCID ProfileManuel Thery, Laurent Blanchoin, View ORCID ProfileAlex Mogilner
doi: https://doi.org/10.1101/437806
Angelika Manhart
1Courant Institute of Mathematical Sciences, New York University, New York, NY 10012, United States
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  • For correspondence: angelika.manhart@cims.nyu.edu mogilner@cims.nyu.edu laurent.blanchoin@cea.fr
Aleksandra Icheva
2CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054 Grenoble, France
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Christophe Guerin
2CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054 Grenoble, France
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Tobbias Klar
2CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054 Grenoble, France
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Rajaa Boujemaa-Paterski
2CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054 Grenoble, France
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Manuel Thery
2CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054 Grenoble, France
3CytomorphoLab, Hôpital Saint Louis, Institut Universitaire d’Hematologie, UMRS1160, INSERM/AP-HP/Université Paris Diderot, 75010 Paris, France
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  • ORCID record for Manuel Thery
Laurent Blanchoin
2CytomorphoLab, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Physiologie Cellulaire & Végétale, Université Grenoble-Alpes/CEA/CNRS/INRA, 38054 Grenoble, France
3CytomorphoLab, Hôpital Saint Louis, Institut Universitaire d’Hematologie, UMRS1160, INSERM/AP-HP/Université Paris Diderot, 75010 Paris, France
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  • For correspondence: angelika.manhart@cims.nyu.edu mogilner@cims.nyu.edu laurent.blanchoin@cea.fr
Alex Mogilner
1Courant Institute of Mathematical Sciences, New York University, New York, NY 10012, United States
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  • ORCID record for Alex Mogilner
  • For correspondence: angelika.manhart@cims.nyu.edu mogilner@cims.nyu.edu laurent.blanchoin@cea.fr
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Abstract

Principles of regulation of actin network dimensions, fundamentally important for cell functions, remain unclear. We studied in vitro and in silico the effect of key parameters, actin density, ADF/Cofilin concentration and network width on the network length. In the presence of ADF/Cofilin, networks reached equilibrium and became globally treadmilling. At the trailing edge, the network disintegrated into large fragments. A mathematical model predicts the network length as a function of width, actin and ADF/Cofilin concentrations. Local depletion of ADF/Cofilin by binding to actin is significant, leading to wider networks growing longer. A single rate of breaking network nodes, proportional to ADF/Cofilin density and inversely proportional to the square of the actin density, can account for the disassembly dynamics. Selective disassembly of heterogeneous networks by ADF/Cofilin controls steering during motility. Our results establish general principles on how the dynamic equilibrium state of actin network emerges from biochemical and structural feedbacks.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted October 08, 2018.
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Reconstitution of the equilibrium state of dynamic actin networks
Angelika Manhart, Aleksandra Icheva, Christophe Guerin, Tobbias Klar, Rajaa Boujemaa-Paterski, Manuel Thery, Laurent Blanchoin, Alex Mogilner
bioRxiv 437806; doi: https://doi.org/10.1101/437806
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Reconstitution of the equilibrium state of dynamic actin networks
Angelika Manhart, Aleksandra Icheva, Christophe Guerin, Tobbias Klar, Rajaa Boujemaa-Paterski, Manuel Thery, Laurent Blanchoin, Alex Mogilner
bioRxiv 437806; doi: https://doi.org/10.1101/437806

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