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Novel use of Diffusion Tensor Imaging to Delineate the Rat Basolateral Amygdala

View ORCID ProfileAndre Obenaus, Eli Kinney-Lang, Amandine Jullienne, Elizabeth Haddad, Duke Shereen, Ana Solodkin, Jeffery F. Dunn, Tallie Z. Baram
doi: https://doi.org/10.1101/439836
Andre Obenaus
1Department of Pediatrics, Loma Linda University School of Medicine, Loma Linda, CA, 92350, USA
2Departments of Anatomy/Neurobiology; University of California Irvine, Irvine, CA, 92697-4475, USA
3Departments of Pediatrics; University of California Irvine, Irvine, CA, 92697-4475, USA
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  • ORCID record for Andre Obenaus
  • For correspondence: obenausa@uci.edu
Eli Kinney-Lang
2Departments of Anatomy/Neurobiology; University of California Irvine, Irvine, CA, 92697-4475, USA
3Departments of Pediatrics; University of California Irvine, Irvine, CA, 92697-4475, USA
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Amandine Jullienne
1Department of Pediatrics, Loma Linda University School of Medicine, Loma Linda, CA, 92350, USA
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Elizabeth Haddad
3Departments of Pediatrics; University of California Irvine, Irvine, CA, 92697-4475, USA
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Duke Shereen
2Departments of Anatomy/Neurobiology; University of California Irvine, Irvine, CA, 92697-4475, USA
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Ana Solodkin
2Departments of Anatomy/Neurobiology; University of California Irvine, Irvine, CA, 92697-4475, USA
4Departments of Neurology, University of California Irvine, Irvine, CA, 92697-4475, USA
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Jeffery F. Dunn
5Department of Radiology, Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, Alberta, T2N 4N1 Canada.
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Tallie Z. Baram
2Departments of Anatomy/Neurobiology; University of California Irvine, Irvine, CA, 92697-4475, USA
3Departments of Pediatrics; University of California Irvine, Irvine, CA, 92697-4475, USA
4Departments of Neurology, University of California Irvine, Irvine, CA, 92697-4475, USA
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Abstract

The amygdaloid complex, including the basolateral nucleus (BLA) contributes crucially to emotional and cognitive brain functions, and is thus a major target of research in both humans and rodents. However, delineating structural amygdala plasticity in both normal and disease-related contexts using neuroimaging has been hampered by the difficulty of unequivocally identifying the boundaries of the BLA. This challenge is a result of poor contrast between BLA and the surrounding gray matter, including other amygdala nuclei. Here we describe a novel DTI approach to enhance contrast, enabling optimal identification of BLA in rodent brain from MR images. We employed this methodology together with a slice-shifting approach to measure BLA volume. We then validated the results by direct comparison to both histological and cellular-identity (parvalbumin)-based conventional techniques for defining BLA in the same brains used for MRI. We also confirmed the BLA region using DTI based tractography. The novel approach used here enables accurate and reliable delineation of BLA. Because this nucleus is involved in, and is changed by, developmental, degenerative and adaptive processes, the instruments provided here should be highly useful to a broad range of neuroimaging studies. Finally, the principles used here are readily applicable to numerous brain regions and across species.

Summary Statement Use of MRI directionally encoded diffusion tensor imaging (DTI) can delineate the basolateral amygdala (BLA) and volumes derived from DTI were found to match those obtained using histological methods. Our approach can be used to identify the BLA.

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Posted October 10, 2018.
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Novel use of Diffusion Tensor Imaging to Delineate the Rat Basolateral Amygdala
Andre Obenaus, Eli Kinney-Lang, Amandine Jullienne, Elizabeth Haddad, Duke Shereen, Ana Solodkin, Jeffery F. Dunn, Tallie Z. Baram
bioRxiv 439836; doi: https://doi.org/10.1101/439836
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Novel use of Diffusion Tensor Imaging to Delineate the Rat Basolateral Amygdala
Andre Obenaus, Eli Kinney-Lang, Amandine Jullienne, Elizabeth Haddad, Duke Shereen, Ana Solodkin, Jeffery F. Dunn, Tallie Z. Baram
bioRxiv 439836; doi: https://doi.org/10.1101/439836

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