Abstract
DNA double-strand breaks are a feature of many acute and long-term neurological disorders, including neurodegeneration, following neurotrauma and after stroke. Persistent activation of the DNA damage response in response to double strand breaks contributes to neural dysfunction and pathology as it can force post-mitotic neurons to re-enter the cell cycle leading to senescence or apoptosis. Mature, non-dividing neurons may tolerate low levels of DNA damage, in which case muting the DNA damage response might be neuroprotective. Here, we show that attenuating the DNA damage response by targeting the meiotic recombination 11, Rad50, Nijmegen breakage syndrome 1 complex, which is involved in double strand break recognition, is neuroprotective in three neurodegeneration models in Drosophila and prevents Aβ1-42-induced loss of synapses in embryonic hippocampal neurons. Attenuating the DNA damage response after optic nerve injury is also neuroprotective to retinal ganglion cells and promotes dramatic regeneration of their neurites both in vitro and in vivo. Dorsal root ganglion neurons similarly regenerate when the DNA damage response is targeted in vitro and in vivo and this strategy also induces significant restoration of lost function after spinal cord injury. We conclude that muting the DNA damage response in the nervous system is neuroprotective in multiple neurological disorders. Our results point to new therapies to maintain or repair the nervous system.
Introduction
DNA double-strand breaks are the most deleterious type of DNA damage. In mitotically cycling cells, double strand breaks trigger the DNA damage response to arrest the cell cycle in and mount repair via non-homologous end-joining in G1 or G2 phases or homologous recombination in M and S phases (Mladenov et al., 2016). Double strand breaks are a feature of many acute and long-term neurological disorders, including many forms of neurodegeneration (Simpson et al., 2015; Merlo et al., 2016), following neurotrauma (Kotipatruni et al., 2011) and after stroke (Hayashi et al., 1998). Unrepaired double strand breaks in neurons leads to persistent activation of the DNA damage response which, in turn, is a trigger for dysregulation of the cell cycle and aberrant re-entry of neurons into G1 leading to neural dysfunction, apoptosis and senescence (Herrup et al., 2004). Re-entry of neurons into the cell cycle and senescence are features of Alzheimer’s disease (McShea et al., 1997; Nagy et al., 1997; Fielder et al., 2017) whilst dysfunctional regulation of the cell cycle and has also been documented in models of cerebral ischemia (Katchanov et al., 2001; Wen et al., 2004) and in post-mortem brain samples of stroke patients (Love, 2003).
If persistent activation of the DNA damage response is a trigger for neuronal dysfunction, apoptosis and senescence then potentially muting the DNA damage response would be neuroprotective. A key sensor and early processor of double strand breaks is the MRN complex, comprising the Mre11, Rad50 and Nbs1/Nbn proteins (Lamarche et al., 2010). Association of the MRN complex to double strand breaks leads to recruitment and activation of the ataxia telangiectasia mutated (ATM) kinase, which coordinates multiple arms of the DNA damage response, including cell-cycle arrest, repair and apoptosis (Shiloh and Ziv, 2013). ATM activation is particularly associated with non-homologous end-joining; in contrast, the related kinase, ataxia telangiectasia and Rad3-related (ATR) protein, regulates homologous recombination. Double strand breaks are characterised by the activation of sensor kinases, including DNA-protein kinase catalytic subunit and regions of DNA damage incorporating phosphorylated histone γH2Ax (Shrivastav et al., 2008). Therefore, γH2Ax is commonly used to monitor direct activation of the DNA damage response in terms of double strand breaks (Celeste et al., 2002; Simpson et al., 2015; Milanese et al., 2018). ATM is recruited at the site of lesions such as double strand breaks to phosphorylate in cis the histone H2Ax (γH2Ax) (Celeste et al., 2002). γH2Ax marks the initiation of a nucleation process resulting in the formation of characteristic γH2Ax+ nuclear foci, which leads to additional ATM recruitment at the damage site, thereby escalating the kinase activity (Shiloh and Ziv, 2013).
In post-mitotic neurons, homologous recombination is unlikely to feature in the repair of double strand breaks as no sister chromatid is available to act as a template for repair. Given its position at the apex of the DNA damage response, the MRN complex is a potential target to attenuate or mute the DNA damage response in neurons. Here, we targeted the MRN complex using both genetic approaches in Drosophila models of neurodegeneration and using small-molecule inhibitors in two rat models of acute neurotrauma: ocular injury and spinal cord injury. We identified that targeting of the MRN complex was neuroprotective in each scenario. This universality opens up new possibilities for treating human neurological disorders.
Materials and Methods
Drosophila stocks and breeding
For all Drosophila experiments except circadian analysis, expression of the neurodegeneration-associated transgenes was restricted to adult neurons by use of an elavC155; GAL80ts driver line. Drosophila were bred on standard yeast/agar media in bottles at 18°C on a 12 h light/dark cycle until eclosion. To induce expression, flies were shifted to and maintained at 29°C, 70% humidity on a 12 h light/dark cycle for the duration of the experiment. Virgin females of the driver line were crossed to males of the control or experimental lines. The control used was an isogenic w1118 strain and all experimental lines were backcrossed over 5 generations into this line. UAS-lines and the rad50EP1 allele were followed during backcrossing by the presence of the w+ transgene and the nbs alleles by PCR. All fly stocks were obtained from the Vienna or Bloomington Drosophila stock centres except for UAS-Aβ1-42 (12-linker) (Speretta et al., 2012), which was a kind gift of Dr. Damien Crowther (University of Cambridge) and UAS-0N4R Tau R406W (Wittmann et al., 2001), which was a kind gift of Dr. Mel Feany (Harvard University).
Quantification of fly movement
Newly eclosed adult flies were separated into cohorts of 20 flies in vials and shifted to 29°C to begin expression by climbing assay or movement tracking.
Negative geotaxis climbing assays
To test climbing, flies were removed from the incubator and allowed to acclimatise for 1 h to the room temperature and humidity. Each vial of flies was tipped into an empty vial and left for 60 s to acclimatise. Flies were tapped to the base and allowed to climb up for 30 s. The percentage of flies climbing above a line 2.5 cm above the base was recorded as the mean of three repeats. Groups of 5 vials were tested together. Flies were transferred to fresh food vials after testing and returned to the incubator. For the Htt.Q128 experiment, second order polynomials were fitted to the data by non-linear regression in Prism 7 and compared by sum-of-squares F-test. For the Aβ1-42 experiment, data were compared by 2-way ANOVA. Significance in both cases was set at p<0.05.
Movement tracking with DART
Flies were housed individually in 65 × 5 mm locomotor tubes (Trikinetics). Tubes were mounted in groups of 20 horizontally on custom platforms and movement recorded from above via a Logitech C920 HD camera mounted on a copy stand. The position of each fly was determined at 5 Hz and movement quantified using DART software running in MATLAB 2017a (Faville et al., 2015). Vibrational stimulation was applied 5 times at 10 min intervals to the flies via motors mounted to the underside of each platform and controlled by the DART software. The mean speed of the population of 20 flies over the 120 s before each stimulation, and the maximum amplitude of the population response to stimulation were quantified by DART. Sigmoidal trend lines were fitted to the data by non-linear regression in Prism 7 and compared by sum-of-squares F-test. A full description of the adaptation of the DART circadian behaviour system to quantify the startle response of flies will be published elsewhere.
Circadian analysis
tim-Gal4 was used to express Aβ1-42 in clock neurons. Flies were maintained at 25°C throughout development and into adulthood in bottles. Newly eclosed adult flies were sorted for genotype and transferred to individual 65 × 5 mm locomotor tubes in DAM activity monitors (Trikinetics) and maintained in 12 h-light/12 h-dark cycle for 3 d then in 12 h-dark/12 h-dark for 10 d. Circadian behaviour was analysed with both spectral analysis using CLEAN and cosinor as described previously (Levine et al., 2002; Rosato and Kyriacou, 2006). Activity in the first day of DD was discarded. Genotypes were compared by ANOVA with a Tukey post-hoc test. Additional flies from the same breeding cohorts were sorted for genotype but maintained in vials in LD to maintain circadian cohesion. After 10 d the brains were dissected at ZT0 (just before lights on) and fixed and stained essentially as described (Dissel et al., 2004) except that the brains were incubated in primary antibodies for 4 d and in secondary antibody overnight (both at 4°C). Primary antibodies used were: mouse anti-PDF C7-s (1:50; Developmental Studies Hybridoma Bank) and rabbit anti-Per1 (1:200; Santa Cruz, CA, USA). Secondary antibodies were: Alexa-488 goat anti-mouse IgG and Cy3 goat anti-rabbit IgG (both 1:100 dilution; ThermoFisher, Leicester, UK). Brains were viewed using an Olympus FV1000 confocal microscope. Per+ cells were counted manually and their identities determined based on position. for statistical analysis, the PDF+ lLNv and sLNv cells were considered together and, similarly, the PDF− DN1, DN2 and LNd cells were pooled. At least 7 brains were used per genotype. Cell numbers were compared by Kruskal-Wallis with a Dunn’s post-hoc test.
Drugs
Mirin and KU-60019 were both purchased from Tocris, Bristol, UK. Mirin is a small molecule inhibitor that blocks the 3’ and 5’ exonuclease activity associated with Mre11 and prevents ATM activation in response to double strand breaks (Dupre et al., 2008; Stivers, 2008; Garner et al., 2009). KU-60019 is a potent ATM kinase inhibitor and has been used to inhibit migration and invasion of human glioma cells in vitro (Golding et al., 2009).
Rat primary hippocampal neuron culture
Primary cultures were prepared from dissected hippocampi of E18 Sprague Dawley rat embryos as previously described (Russell et al., 2012). Cells were plated at a density of either 75,000 or 500,000 cells on poly-D-lysine (Sigma, Poole, UK; 0.1 mg/ml in borate buffer, pH 8.5) coated glass cover slips or 6-well plates respectively. The plating medium was DMEM supplemented with 5% FBS, penicillin/streptomycin (P/S) and 0.5 mM L-glutamine (all from ThermoFisher). On the next day, the medium was changed to Neurobasal medium supplemented with B27, P/S and 0.5 mM L-glutamine (ThermoFisher). Cultures were incubated at 37°C and 5% CO2 and were used between 18 to 21 days in vitro.
Rat primary DRGN cultures
Primary adult rat DRGN were prepared as described previously (Ahmed et al., 2005). DRGN cells were cultured in Neurobasal-A (ThermoFisher) at a plating density of 500 DRGN/well in chamber slides (Beckton-Dickinson, Watford, UK) pre-coated with 100 μg/ml poly-D-lysine. In preliminary experiments, the optimal concentration of mirin (100 μM) and KU-60019 (10 μM) that promoted DRGN survival and neurite outgrowth were determined. The positive control was pre-optimised fibroblast growth factor (FGF)-2 (Peprotech, London, UK; 10 ng/ml (Ahmed et al., 2005)). Cells were cultured for 4 days in a humidified chamber at 37 °C and 5% CO2.
Rat primary retinal cultures
Primary adult retinal cultures, containing enriched populations of retinal ganglion cells (RGC) were prepared as described previously (Ahmed et al., 2006b). Briefly, retinal cells were dissociated into single cell suspensions using a Papain dissociation kit (Worthington Biochemicals, New Jersey, USA). 125 × 103 retinal cells/well were cultured in chamber slides (Beckton-Dickinson) pre-coated with 100 μg/ml poly-D-lysine. In preliminary experiments, the optimal concentration of mirin (100 μM) and KU-60019 (10 μM) that promoted RGC survival and neurite outgrowth were determined. The positive control was pre-optimised CNTF (Peprotech; 20 ng/ml (Douglas et al., 2009)). Cells were cultured for 4 days in a humidified chamber at 37°C and 5% CO2.
Preparation of Aβ oligomers and neuronal treatment
Preparation of Aβ peptides and treatment of neurons has been previously described (Marsh et al., 2017). Briefly, Aβ1-42 (Bachem) was prepared by dissolving the peptide in DMSO (1 mM). The reconstituted peptides were diluted in 10 mM Tris, pH7.8 at a working concentration of 300 μM and stored in aliquots at −80 °C. Peptides were diluted to working concentration in full Neurobasal medium after thawing and used immediately. Neurons were incubated with 0.5 μM Aβ1-42 or 0.5 μ Aβ1-42 + 100 μM mirin for 24 h.
Immunocytochemistry
Adult fly brains were dissected in PBS and fixed for 20 min in 4% formaldehyde in PBS. After washing, brains were blocked in 1% BSA in PBS for 1 h then incubated with rat anti-Elav (Developmental Studies Hybridoma Bank clone 7E8A1, 1:25) and mouse anti-phosphorylated pH2Av (DSHB clone UNC93-5.2.1, 1:25) for 48 h at 4°C. Brains were washed in PBS + 0.3% triton X-100 for 2 h at RT then incubated in cross-adsorbed Alexa-594 anti-rat and Alexa-488 anti-mouse secondary antibodies (Jackson ImmunoResearch, both 1:400) for 48 h at 4°C. After washing as before, brains were mounted on bridge slides in Vectashield (Vector Labs).
Hippocampal cells on coverslips following treatment, were rinsed once with pre-warmed (37 °C) PBS and fixed with 4% paraformaldehyde (PFA; TAAB, Peterborough, UK), 2% Sucrose in PBS. Fixed neurons were washed with TBS, permeabilised with 0.1% Tween-20 and 5% horse serum in TBS for 45 min at room temperature and incubated with primary antibody overnight at 4 °C. Coverslips were mounted using the ProLong Gold reagent (ThermoFisher). The antibodies used were: mouse anti-H2Ax pSer319 (γH2Ax; JBW301; 1;1000 dilution; Merck) and Alexa-568 anti-mouse IgG (ThermoFisher). The cells were visualized on a spin disc confocal system (CARV from Digital Imaging Solutions) with an EM-CCD camera (Rolera/QI Cam 3500) mounted on an Olympus X71 microscope, using a 100x fluoplan objective (NA 4.2). The microscope confocal system was supported by Image Pro 6.0 software.
DRGN and RGC were fixed in 4% paraformaldehyde, washed in 3 changes of PBS before being subjected to immunocytochemistry as described previously (Ahmed et al., 2005; Ahmed et al., 2006b). Antibodies used were: mouse anti-βIII tubulin (1:200 dilution; Sigma) to visualise neuronal cell soma and neurites and Alexa-488 goat anti-mouse IgG (1:400 dilution; ThermoFisher). Slides were then viewed with an epifluorescent Axioplan 2 microscope, equipped with an AxioCam HRc and running Axiovision Software (all from Zeiss, Hertfordshire, UK). The proportion of DRGN with neurites and the mean neurite length were calculated using Axiovision Software by an investigator masked to the treatment conditions as previously described (Ahmed et al., 2005; Ahmed et al., 2006b). All experiments were performed in triplicate and repeated on 3 independent occasions.
In vivo surgical procedures
Experiments were licensed by the UK Home Office and all experimental protocols were approved by the University of Birmingham’s Animal Welfare and Ethical Review Board. All animal surgeries were carried out in strict accordance to the guidelines of the UK Animals Scientific Procedures Act, 1986 and the Revised European Directive 1010/63/EU and conformed to the guidelines and recommendation of the use of animals by the Federation of the European Laboratory Animal Science Associations (FELASA). Animals were housed in a standard facility and kept on a 12-h light–12-h dark cycle, with a daytime luminance of 80 lux, fed and watered ad libitum. For all in vivo experiments, adult female Sprague-Dawley rats weighing 170-220g (Charles River, Margate, UK) were used. Rats were randomly assigned to each experimental group with the investigators masked to the treatment conditions.
Optic nerve crush injury (ONC)
Rats were injected subcutaneously with 50 µl Buprenorphine to provide analgesia prior to surgery and anaesthetised using 5% of Isoflurane in 1.8 ml/l of O2 with body temperature and heart rate monitored throughout surgery. ONC was performed 2mm from the lamina cribrosa using watchmaker’s forceps (Ahmed et al., 2005; Berry et al., 1996).
In pilot dose-finding experiments, mirin and KU-60019 were intravitreally injected at 1, 2, 2.5, 5, 7.5 and 10 µg (n = 3 rats/group, 2 independent repeats), without damaging the lens, immediately after ONC and every other day, or twice weekly or once every 7 days, in a final volume of 5µl saline for 24 days (not shown). Rats were then killed and retinae were dissected out, lysed in ice-cold lysis buffer, separated on 12% SDS PAGE gels and subjected to western blot detection of γH2Ax levels. We determined that the dosing frequency of twice weekly and 2.5 and 5 µg of mirin and KU-60019 optimally reduced γH2Ax levels, respectively. Twice weekly intravitreal injections were sufficient in the eye since the vitreous may acts as a slow release gel due to its composition, being made of mainly water, collagen type II fibrils with glycosaminoglycans, hyaluronan and opticin. Optimal doses were then used for all experiments described in this manuscript. Rats were killed in rising concentrations of CO2 at 1 and 24 days after optic nerve crush injury for western blot analyses or at 24 days after ONC for determination of RGC survival and axon regeneration, as described below.
For the experiments reported in this manuscript, n = 6 rats/group were used and assigned to: (1), Intact controls (no surgery to detect baseline parameters); (2), ONC+vehicle (ONC followed by intravitreal injection of vehicle solution; to detect surgery-induced changes); (3), ONC+mirin (ONC followed by intravitreal injection of 2.5µg of mirin, twice weekly; to monitor effects of inhibiting Mre11); and (4), ONC+KU-60019 (ONC followed by intravitreal injection of 5µg of KU-60019; to monitor effects of inhibiting ATM). FluoroGold was injected into the proximal nerve stump 2d before sacrifice and whole retinal flatmounts were used to assess RGC survival in vivo, as described by us previously (Ahmed et al., 2011). Each experiment was repeated on 3 independent occasions with a total n = 18 rats/group/test.
DC crush injury
For the DC lesion model, experiments also comprised n = 6 rats/group: (1), Sham controls (Sham; to detect surgery-induced changes; partial laminectomy but no DC lesion); (2), DC transected controls + intrathecal injection of vehicle (PBS); (3), (DC+vehicle; to detect injury-mediated changes); (4), DC+intrathecal injection of mirin (DC+mirin; to monitor effects of inhibiting Mre11); (5), and DC+intrathecal injection of KU-60019 (DC+KU-60019; to monitor effects of inhibiting ATM). Each experiment was repeated on 3 independent occasions with a total n = 18 rats/group/test.
Rats were injected subcutaneously with 50 μl Buprenorphine to provide analgesia prior to surgery and anaesthetised using 5% of Isoflurane in 1.8 ml/l of O2 with body temperature and heart rate monitored throughout surgery. For optic nerve crush injury, a supraorbital approach was used to expose the optic nerve and crushed with calibrated watchmaker’s forceps 2mm from the lamina cribrosa of the eye (Berry et al., 1996). Animals were immediately injected intravitreally and avoiding damaging the lens with vehicle, mirin or KU-60019. Animals were then allowed to survive for 24 days before assessment of RGC survival and axon regeneration. For the DC injury model, a partial T8 laminectomy was performed and the DC were crushed bilaterally using calibrated watchmaker’s forceps as described (Surey et al., 2014; Almutiri et al., 2018). The subarachnoid space was cannulated with a polyethylene tube (PE-10; Beckton Dickinson) through the atlanto-occipital membrane as described by others (Yaksh and Rudy, 1976). The catheter tip was advanced 8 cm caudally to the L1 vertebra and the other end of the catheter was sealed with a stainless-steel plug and affixed to the upper back. Animals were injected immediately with vehicle (PBS), mirin or KU-60019 followed by a 10μl PBS catheter flush. Injections were repeated every 24 h and drugs and vehicle reagents were delivered over 1 min time period using a Hamilton microlitre syringe (Hamilton Co, USA).
In a pilot experiment, mirin and KU-60019 were injected as described above at 1, 2, 5, 10 and 15 μg (n = 3 rats/group, 2 independent repeats) in a final volume of 10μl saline either daily, every other day or twice weekly for 28 d (not shown). Rats were then killed and the lesion site plus 5mm either side were harvested, lysed in ice cold lysis buffer, separated on 12% SDS PAGE gels and subjected to western blot detection of γH2Ax levels (Surey et al., 2014). We determined that the amount of mirin and KU-60019 to optimally reduce γH2Ax levels by intrathecal delivery was 5 and 10 μg, respectively with a dosing frequency of every 24hrs. Optimal doses were then used for all experiments described in this manuscript. Rats were killed in a rising concentration of CO2 at either 28 d for immunohistochemistry and western blot analyses or 6 weeks for electrophysiology and functional tests.
Knockdown of Mre11 using shRNA in vivo
SMARTvector Lentiviral shRNAs to Mre11 (shMre11; cat no. V3SR11242-240192989) and ATM (shATM; cat no. V3SR11242-238270626) under the control of a CMV promoter were purchased from Dharmacon and plasmid DNA was prepared from glycerol stocks according to the manufacturer’s instructions. A control plasmid containing the CMV promoter and a non-targeting control (shControl; cat no. VSC11721) was also purchased from Dharmacon. Plasmid DNA containing shMre11, shATM and shControl were complexed with in vivo-jetPEI (referred to as PEI from herein; Polyplus Transfection, New York, USA) according to the manufacturer’s instructions and injected into the DRG immediately after DC injury, as described by us previously (Jacques et al., 2012; Almutiri et al., 2018). We used in vivo-jetPEI, a non-viral vector, to deliver the shRNAs to DRGN in vivo, since we have previously shown that it transduces similar proportions of DRGN as adeno-associated virus 8 and that it does not require intra-DRG injection 1-2 week prior to injury to ensure maximum transgene expression (Almutiri et al., 2018). In a preliminary experiment, we optimized the amount of plasmid DNA required to cause maximum suppression of γH2Ax+ foci in DRGN as 2μg for both shMre11 and shATM (not shown). Pre-optimised plasmid DNA was then injected intra-DRG and animals (n = 6/group/test, 3 independent repeats, total n = 18 rats/group/test) were killed after 28d for immunohistochemistry to detect γH2Ax+ or 6 weeks for electrophysiological and behavioural studies.
Immunohistochemistry
Tissue preparation for cryostat sectioning and immunohistochemistry were performed as described previously (Surey et al., 2014). Briefly, rats were intracardially perfused with 4% formaldehyde and optic nerves, eyes, L4/L5 DRG and segments of T8 cord containing the DC injury sites were dissected out and post-fixed for 2h at room temperature. Tissues were then cryoprotected in a sucrose gradient prior to mounting in optimal cutting temperature (OCT) embedding medium (ThermoFisher) and frozen on dry ice. Samples were then sectioned using a cryostat and immunohistochemistry was performed on sections from the middle of the optic nerve, DRG or spinal cord as described previously (Surey et al., 2014). Sections were permeabilised in PBS containing 0.1 % Triton X-100, blocked in PBS + 3% w/v bovine serum albumin + 0.05% Tween-20 then stained with primary antibodies overnight at 4 °C. After washing in PBS, sections were incubated with secondary antibodies for 1 h at RT then washed further in PBS and mounted in Vectashield containing DAPI (Vector Laboratories). Primary antibodies used were: mouse anti-H2Ax pSer139 (γH2Ax; JBW301; 1:400 dilution; Merck) and rabbit anti-neurofilament (NF) 200 (1:400 dilution; Sigma); mouse anti-GAP43 (ThermoFisher; 1:400 dilution) was used to detect regenerating axons in the optic nerve and spinal cord. Regenerating axons in the DC were detected using GAP43 immunohistochemistry (Ahmed et al., 2014; Almutiri et al., 2018; Farrukh et al., 2019) since Cholera toxin B labelling in our hands did not label regenerating axons by retrograde transport labelling in the rat (Ahmed et al., 2014), despite others demonstrating successful labelling (Neumann and Woolf, 1999; Neumann et al., 2002). Secondary antibodies used were Alexa-488 goat anti-mouse IgG and TexasRed goat anti-rabbit IgG (both from ThermoFisher). Controls were included in each run where the primary antibodies were omitted and these sections were used to set the background threshold prior to image capture. Image capture and analysis was performed by an investigator masked to the treatment conditions.
Quantification of axon regeneration
Axon regeneration in the spinal cord was quantified according to previously published methods (Hata et al., 2006). Briefly, serial parasagittal sections of cords were reconstructed by collecting all serial 50μm-thick sections (~70–80 sections/animal; n = 10 rats/treatment) and the number of intersections of GAP43+ fibers through a dorsoventral orientated line was counted from 6mm rostral to 4mm caudal to the lesion site. Axon number was calculated as a % of fibers seen 4mm above the lesion, where the DC was intact.
Electrophysiology
Six weeks after surgery or treatment, compound action potentials (CAP) were recorded after vehicle, mirin and KU-60019 treatment as previously described (Lo et al., 2003; Hains et al., 2004; Almutiri et al., 2018). Briefly, with the experimenter masked to the treatment conditions, silver wire electrodes were used apply single-current pulses (0.05ms) through a stimulus isolation unit in increments (0.2, 0.3, 0.6, 0.8, and 1.2 mA) at lumbar (L)1-L2 and compound action potentials (CAP) recorded at cervical (C)4-C5 along the surface of the midline spinal cord. CAP amplitudes were calculated between the negative deflection after the stimulus artefact and the next peak of the wave. CAP area was calculated by rectifying the negative component (full-wave rectification in Spike 2 software) and measuring its area at the different stimulation intensities. The dorsal half of the spinal cord was transected between stimulating and recording electrodes at the end of the experiment to confirm that a CAP could not be detected. Electrophysiology was analysed using Spike2 software (Cambridge Electronic Design, Cambridge, UK) and representative processed data are shown for CAP traces.
Functional tests after DC injury
Functional testing after DC lesions was carried out as described by previously (Fagoe et al., 2016; Almutiri et al., 2018). Briefly, animals (n = 6 rats/group, 3 independent repeats (total n = 18/group/test)) randomly assigned and treatment status masked from the investigators, received training to master traversing the horizontal ladder for 1 week before functional testing. Baseline parameters for all functional tests were established 2-3 d before injury. Animals were then tested 2 d after DC lesion + treatment and then weekly for 6 weeks. Experiments were performed by 2 observers blinded to treatment with animals tested in the same order and at the same time of day. Three individual trials were performed each time for each animal.
Horizontal ladder crossing test
This tests the animals’ locomotor function and is performed on a 0.9-meter-long horizontal ladder with a diameter of 15.5 cm and randomly adjusted rungs with variable gaps of 3.5-5.0 cm. Animals were assessed traversing the ladder with the total number of steps taken to cross the ladder and the number of left and right rear paw slips being recorded. The mean error rate was then calculated by dividing the number of slips by the total number of steps taken.
Tape removal test (sensory function)
The tape removal test determines touch perception from the left hind paw. Animals were held with both hind-paws extended and the time it took for the animal to detect and remove a 15×15mm piece of tape (Kip Hochkrepp, Bocholt, Germany) was recorded and used to calculate the mean sensing time.
Analysis of functional tests
The whole time-course of lesioned and sham-treated animals for the horizontal ladder crossing and mean tape sensing/removal test was compared using generalized linear mixed models (GLMM) or linear mixed models (LMM), as described previously (Fagoe et al., 2016; Almutiri et al., 2018). For the horizontal ladder test, we scored individual steps as either a successful step or a slip and therefore the data follows a binomial distribution. Data was compared using binomial GLMM, with lesioned/sham (‘LESION’; set to true in lesioned animals post-surgery, false otherwise) and operated/unoperated (‘OPERATED’; set to false before surgery, true after surgery) as fixed factors, animals as a random factors and time as a continuous covariate. Binomial GLMMs were then fitted in R using package lme4 with the glmer function using the following model formulae (Fagoe et al., 2016):
Bracketed terms refer to the ‘random effects’ and account for the presence of repeated measurements in estimation of the effect size and significance of INT and LESION. ‘Outcome’ for binomial GLMMs is a two-column list of counts of successes/fails per run as described in the individual models above. INT refers to the interaction term of LESION over time (Model 1). P-values for INT were then calculated, which represents differences in the evolution of outcomes over time, and LESION, the unconditional main effect of lesioning. Significance of specific parameters in LMMs and GLMMs can be determined by comparing a model containing the parameter of interest to a reduced model without it. Thus, INT was assessed by comparing Model 1 and Model 2, while LESION was assessed by comparing Model 3 and Model 4. LESION, represents the overall effect of lesioning on the outcome, while INT represents the difference in slope of the outcome between the two groups, i.e. speed of recovery. P-values for GLMMs were calculated by model comparison using parametric bootstrap for INT and LESION against the null hypothesis that each parameter is zero, using pbkrtest in R package. Between 1000 and 20000 simulations were used.
For tape removal test, the time-courses of lesioned versus sham were compared using linear mixed models (LMMs) with the R package lme4 with the glmer function (Fagoe et al., 2016; Almutiri et al., 2018). Model formulae were the same as for the ladder crossing test above. Standard regression diagnostics (quantile plots of the residuals vs the normal distribution, plots of residuals vs fitted values) were carried out for the data fitted with LMMs. P-values for the INT and LESION parameters of the LMMs were calculated by model comparison using package pbkrtest in R, with the Kenward-Roger method. For the tape sensing and removal test, log of the withdrawal time was used as the data were expected to follow an exponential distribution. Independent sample T-tests were performed to determine statistical differences at individual time points.
Analysis of pH2A positive foci
Drosophila brains
Adult fly brains were dissected in PBS and fixed and stained as described above. 41 × 41 × 13.5μm volumes of the same region of the central brain were imaged at 16-bit depth for each brain using a 63x water immersion N.A. 1.2 objective on a Zeiss LSM880 confocal microscope. The zoom was set at 3.2 and z-step at 0.45 μm and Airy scan processing module used with super-resolution settings to improve spatial resolution post-collection. To display visualise brightly-staining pH2Av+ foci only, the lower intensity pan-nuclear staining was eliminated by manually resetting the black level threshold in Fiji-3. The identical original and processed images are displayed together in supplementary figures. Movies were rendered in Fiji-3.
DRGN
Frequency distribution: Images of the entire DRG in the middle 3 sections (n = 160 images/DRG) of L4/L5 DRG from each animal (n = 10) were captured by an investigator masked to the treatment conditions at x10 magnification using a Zeiss Axioplan 200 epifluorescent microscope. Images were merged in Adobe Photoshop using Photomerge and the frequency of γH2Ax+ foci in different diameter DRGNs was recorded (Jacques et al., 2012).
Relative fluorescent staining intensity: the integrated density of fluorescence was measured using ImageJ as previously described (Surey et al., 2014). Briefly, images from the middle 3 DRG sections from n = 10 rats were thresholded and the mean integrated density of pixels/cell was recorded.
Western blot: Total protein was extracted from pooled L4/L5 DRG pairs from n = 3 rats/group (i.e. 6 DRG) after DC+vehicle, mirin and KU-60019 treatment and western blots followed by subsequent densitometry was performed as described by us previously and in brief below (Ahmed et al., 2005). Experiments were repeated on 3 independent occasions (total n = 9 rats/group i.e. 18 DRG/group).
Western blots
Proteins were extracted from hippocampal, retinal or DRG neuron cultures/tissues as described by us previously (Ahmed et al., 2005). Briefly, cultures were treated with ice-cold lysis buffer (20mM HEPES pH 7.5, 1 mM EDTA, 150 mM NaCl, 1% NP-40 and 1 mM dithiothreitol supplemented with protease (Roche, Welwyn Garden City, UK) and phosphatase (ThermoFisher) inhibitor cocktails), protein concentration was determined by Bradford Protein Assay and 15 μg of total protein separated on 12% Tris-glycine SDS-PAGE gels. Proteins were transferred to nitrocellulose membranes, blocked with 2.5% non-fat dry milk in TBS containing 0.05% Tween-20 for 90 min with agitation then incubated for 2 h with primary antibodies and for 1 h with secondary antibodies diluted in 2.5% non-fat dry milk at RT. For hippocampal neurons, proteins were detected using the Odyssey imaging scanner (LI-COR). Primary antibodies used were: mouse anti-Synapsin antibody (Santa Cruz) and mouse anti-actin antibody (Sigma, A5316). Secondary antibodies used were: Alexa-680 goat anti-mouse IgG and Alexa-800 goat anti-rabbit IgG (New England Biolabs). For DRGN cultures, membranes were probed with primary anti-γH2Ax (pSer139 monoclonal antibody; 1:400 dilution; Merck) and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma: 1:1000 dilution) was used as a protein loading control for western blots.
Densitometry
Western blots were scanned into Adobe Photoshop (Adobe Systems, San Jose, CA, USA) keeping all scanning parameters constant between blots. The integrated density of bands was analysed using the built-in macros for gel analysis in ImageJ as described by us previously (Ahmed et al., 2006a).
Statistical analysis
All data are presented as means ± standard error of the mean (SEM). Comparison of means were performed using one-way or two-way ANOVA using Prism 7 (San Diego, CA, USA) or SPSS (Version 25, IBM, New York, USA). Tukey’s significant difference test followed ANOVA’s, as appropriate. In Fig. 2A a Kruskal-Wallis test with Dunn’s post-hoc was performed. Functional tests in rats were analysed using R package (www.r-project.org) as described above. Tests used for each experiment are described in the corresponding methods. In all tests, P<0.05 were considered statistically significant. Supplementary Table 1 lists all tests performed, the comparisons made, and the associated P-values.
Data availability
The authors confirm that the data supporting the findings of this study are available within the article and its Supplementary material.
Results
Genetic targeting of the double strand break-sensing MRN complex is neuroprotective in Drosophila. Double strand breaks are a feature of early-stage Alzheimer’s disease and correlate with reduced cognitive score (Simpson et al., 2015). Double strand breaks are also generated in neurons in vivo exposed to Aβ1-42 oligomers (Suberbielle et al., 2013). Hence, we chose to target the MRN complex in a Drosophila model of Alzheimer’s model as proof-of-concept. In this model, tandem Aβ1-42 oligomers for Alzheimer’s disease (AD) were expressed in, and secreted from, adult post-mitotic neurons to replicate the extracellular deposition in AD (Speretta et al., 2012). Expression was restricted to adult neurons only to rule out confounding developmental phenotypes (see Methods for genetics). First, we confirmed that double strand breaks were generated in neurons exposed to A oligomers, as predicted from other studies and our own studies in rat hippocampal neurons (Fig. 2). Activation and recruitment of ATM to the site of double strand breaks lead to multiple phosphorylation events, one of which is the phosphorylation of histone γH2Ax at and around the site of the break, resulting in the formation of characteristic γH2Ax+ nuclear foci (Shrivastav et al., 2008). Therefore, antibodies to γH2Ax (H2Av in Drosophila) are widely use to visualise the histone modifications surrounding a double strand break (Celeste et al., 2002; Simpson et al., 2015; Milanese et al., 2018). Anti-pH2Av staining of control brains revealed neurons with a single brightly staining focus per nucleus corresponding to the nucleolus organizing centre. In contrast, many of the Aβ1-42-expressing neurons had multiple, clustered foci (Fig. 1A, Supplementary Fig. 1 and Movie 1).
Next, we asked whether reducing nbs levels genetically would be neuroprotective in Aβ1-42 expressing flies. We chose to introduce one null allele of nbs to reduce the gene dosage by 50% and then used the ability of flies to climb after being startled as a measure of neural output. This negative geotaxis assay is widely used in Drosophila neurodegeneration studies: climbing ability declines rapidly and correlates with a reduced speed of neural transmission (Kerr et al., 2011). In flies expressing Aβ1-42 oligomers, climbing ability decreased rapidly with age yet this was partially suppressed in nbs−/+ heterozygous flies (Fig. 1B and see Movie 2). The toxicity of the less aggregative tAβ1-42 construct lacking the 12 amino acid flexible linker was similar suppressed in nbs1/+ heterozygous flies and a second null allele, nbs2/+ showed a similar effect (Supplementary Fig. 2A and B).
To confirm that the neuroprotective effect was not specific to Aβ1-42 pathology, we used the same approach for flies expressing an expanded Htt protein associated with Huntington’s disease (Htt.Q128) (Romero et al., 2008) and saw a similar neuroprotective effect in climbing assays (Fig. 1C). Again, to confirm the suppression was not specific to climbing ability, we switched to a tracking system capable of monitoring the horizontal movement of flies continuously and expressed the human 0N4R Tau carrying the R406W point mutation associated with frontal temporal dementia with Parkinsonism (Wittmann et al., 2001). Tau-expressing flies moved at normal speed when unstimulated (not shown) but their escape response declined rapidly with age. Again, this was partially suppressed in nbs−/+ flies (Fig. 1D) and this neuroprotective effect was not due to a reduction in Tau levels nor to changes in Tau phosphorylation in the brains of these flies (Supplementary Fig. 2C). Finally, to ensure these protective effects were not specific to nbs, we depleted rad50 in Htt.Q128-expressing flies using the same strategy of reducing the gene dosage with a null allele. Flies with reduced rad50 (rad50−/+) partially suppressed the decline in climbing ability in the Htt.Q128 flies (Supplementary Fig. 2D). We also expressed expanded HttQ128 in the developing eye under the control of GMR-gal4, which generates a progressive degeneration evidenced as a widespread loss of pigmentation after 6-7 weeks (Romero et al., 2008). In rad50−/+ flies, pigmentation is restored, indicating enhanced cellular survival (Supplementary Fig. 2E).
Targeting the MRN complex suppresses neurodegeneration-relevant phenotypes
AD patients commonly suffer disrupted circadian behaviour patterns (Saeed and Abbott, 2017) and a similar effect is observed in Aβ-expressing flies (Long et al., 2014). We asked whether reducing nbs genetically would suppress this more directly disease-relevant phenotype. Tandem Aβ1-42 was expressed specifically in clock neurons under the control of tim-Gal4 and their survival studied with immunocytochemistry (Renn et al., 1999). Aβ-expression induced loss of some Per+ neurons but this was partially suppressed in nbs−/+ flies (Fig. 2A). Consistent with the loss of clock neurons (Renn et al., 1999), Aβ-expressing flies exhibited significantly longer free-running circadian locomotor cycles than controls but the normal ∼24 h periodicity was restored in nbs−/+ flies (Fig. 2B and Supplementary Fig. 2F).
We extended our findings to mammalian neurons by examining synapse loss – a common early feature of neurodegenerative diseases (Gillingwater and Wishart, 2013). Cultured primary hippocampal neurons were exposed to Aβ1-42 oligomers and stained with anti-γH2Ax antibodies. As expected, the characteristic foci corresponding to double strand breaks in the nuclei were induced by exposure to Aβ1-42 (Fig. 2C). Synapsin levels were significantly reduced 24 h after addition of Aβ oligomers (Fig. 2D) suggesting that one route to synapse loss in AD might be via Aβ-induced double strand breaks triggering the DNA damage response. Mirin, a small-molecule Mre11 exonuclease inhibitor (Dupre et al., 2008), prevented synapsin loss (Fig. 2D), indicating that targeting the MRN complex can also protect mammalian neurons from changes associated with early-stage neurodegeneration.
Mre11 or ATM inhibition prevents apoptosis and stimulates regeneration after neurotrauma
Double strand breaks are a feature of acute neurological disorders (Hayashi et al., 1998; Kotipatruni et al., 2011). DNA strand breaks have been observed in retinal ganglion cells (RGC) after optic nerve injury (He et al., 2012) and neurons suffering from ischemia display genome fragility and fragmented DNA both in vitro and in vivo (Yang et al., 2016). We used an adult rat primary retinal culture system enriched in RGC, where cells are grown in the presence of inhibitory CNS myelin extracts to simulate a post-injury environment (Ahmed et al., 2006b). As expected, we detected the presence of double strand breaks with anti-γH2Ax antibodies in the RGC (Fig. 3A and B). The RGC die rapidly by apoptosis and, even if apoptosis is blocked with inhibitors, they fail to regenerate neurites (Vigneswara et al., 2013). We treated the RGC with mirin to attenuate the DNA damage response. This blocked RGC apoptosis and dramatically stimulated neurite regrowth. Mirin treatment was significantly more effective than a current positive control treatment: ciliary neurotrophic factor (CNTF) (Fig. 3C and D). Since a key function of the MRN complex is to recruit and activate ATM at double strand breaks (Lee and Paull, 2005), and given that HD-pathology is reduced by targeting ATM (Lu et al., 2014), we asked whether inhibiting ATM directly would also be neuroprotective to RGC. Treatment with the highly selective ATM inhibitor, KU-60019, had similarly dramatic effects on RGC survival and neurite outgrowth as mirin (Fig. 3C-F).
We extended our in vitro findings to an in vivo optic nerve crush injury paradigm. We observed low levels of γH2Ax in intact control rat retinae in vivo (Fig. 4A and B). However, high levels of γH2Ax were observed within 1 d and at 24 d after optic nerve crush injury, suggesting rapid activation of double strand breaks and persistence of high levels for the duration of the experiment i.e. 24 days. Thus, the DNA damage response pathway was persistently activated in the retina post-injury. We used a pre-optimized dosing regimen for mirin and KU-60019 for 24 days after optic nerve crush injury to attenuate the DNA damage response, as demonstrated by significantly suppressed γH2Ax in western blots (Fig. 4C) and subsequent densitometry (Fig. 4D) and saw unprecedented RGC survival of 93% and 91%, respectively, when compared to intact controls (Fig. 4E and F). Both mirin and KU-60019 also promoted significant numbers of RGC to regenerate their axons, which emerged from the lesion site and grew for long distances (Fig. 4G and H). The numbers of regenerating axons we see are, to the best of our knowledge, unprecedented when compared to known treatments and the axons also regenerate longer distances into the distal optic nerve than has been seen previously (Berry et al., 1996; Leon et al., 2000; Vigneswara et al., 2013).
Mre11 and ATM inhibitors promote DRGN survival and neurite outgrowth in vitro and DC axon regeneration after spinal cord injury in vivo
Double strand breaks are also generated in spinal neurons after spinal cord injury (SCI) (Kotipatruni et al., 2011) and there is an urgent need for effective therapies to treat SCI patients. Initially, we tested our DNA damage response attenuation strategy using a similar in vitro culture model to the retina in which dorsal root ganglion neurons (DRGN) containing double strand breaks are cultured from adult rats and grown in the presence of inhibitory CME (Supplementary Fig. 3A). Again, mirin and KU-60019 treatment stimulated significant DRGN survival and promoted neurite outgrowth (Supplementary Fig. 3B-E).
To generate a spinal cord injury in vivo, we surgically injured the ascending long tract axons of the dorsal funiculus in adult rats by dorsal column (DC) crush injury (Surey et al., 2014) and mirin or KU-60019 inhibitors were administered through an intrathecal catheter. None of the animals showed adverse effects to SCI and all animals were included for analysis. Double strand breaks formed in DRGN of all diameters after DC+vehicle treatment, as predicted from previous studies (Kotipatruni et al., 2011) (Fig. 5A and B). However, quantification of the intensity of γH2Ax+ was significantly reduced in DC+mirin and DC+KU-60019-treated animals (Fig. 5C). Similarly, western blot and subsequent quantification for γH2Ax levels showed significant attenuation in DC+mirin and DC+KU-60019-treated rats, indicating partial suppression of the DNA damage response (Fig. 5D and E).
We next investigated if suppression of DNA damage response by mirin and KU-60019 promotes DC axon regeneration after injury in rats. Little or no GAP43+ immunoreactivity was detected in DC+vehicle-treated rats whilst a large cavity was present at the lesion site (#) (Fig. 6A). Quantification of the % of axons in DC+vehicle-treated rats falls rapidly to 0% at the lesion site (i.e. 0mm) and remains at 0% for all distances quantified (Fig. 6B). In DC+mirin and DC+KU-60019-treated rats, significant GAP43+ immunoreactivity was present beyond the lesion cavity and in the rostral segment of the spinal cord (Fig. 6A and B). Quantification of the % of axons demonstrated significantly enhanced numbers at all distances when compared to DC+vehicle-treated rats, with DC+KU-60019 treatment regenerating slightly greater numbers of GAP43+ axons at all distances rostral to the lesion (Fig. 6B). For example, DC+KU-60019 treatment regenerated 47 ± 8, 35 ± 6, 25 ± 4 and 18 ± 3% of axons at 0, 2, 4 and 6mm rostral to the lesion site, compared to DC+vehicle-treated rats (Fig. 6B).
Mre11 and ATM inhibitors restore function after spinal cord injury in vivo
We employed electrophysiology and simple functional tests (Almutiri et al., 2018) to quantify recovery from the injury after mirin or KU-60019 treatment. In DC+vehicle-treated rats, the normal compound action potential (CAP) trace across the lesion site in Sham controls was abolished, whilst treatment with either mirin and KU-60019 restored 50-55% of the CAP trace (Fig. 7A) and the CAP amplitude (Fig. 7B) observed in sham-treated animals. As expected, dorsal hemisection between the stimulating and the recording electrodes at the end of the experiment abolished CAP traces in all animals (if a CAP trace was observed), confirming that the experiment was technically successful (Fig. 7A). The CAP area was also significantly improved in mirin and KU60019-treated rats compared to vehicle groups (Fig. 7C). The improved electrophysiological function translated into very dramatic improvements in sensory and locomotor function. We used a tape sensing-and-removal test and a horizontal ladder-crossing test to quantify recovery of sensory and motor function, respectively (Almutiri et al., 2018). In sham-treated animals, there is a small initial decline in mean sensing/removal time (Fig. 7D) and mean ratio of slips to total steps (Fig. 7E) due to the effects of anaesthetic but these are restored to baseline levels within 3 weeks. Vehicle-treated groups displayed significantly impaired sensory and motor function that failed to return to baseline levels even after 6 weeks. In contrast, mirin or KU-60019-treated groups both showed dramatically improved sensing times and ladder-crossing ability 2d after surgery and after 3 weeks were indistinguishable from the sham-treated control. These results demonstrate that either Mre11 or ATM inhibition is able to promote functional recovery after SCI in vivo, which we believe to be at unprecedented levels.
Finally, we confirmed the results obtained with the inhibitors using specifically designed shRNA plasmids targeting Mre11 (shMre11) and ATM (shATM). These shRNA plasmids were delivered using a non-viral gene delivery vector, in vivo-JetPEI (PEI), which we have shown to be as efficient as AAV8 in transducing DRGN after Intra-DRG injection in vivo and does not invoke an off-target immune response (Almutiri et al., 2018). We initially demonstrated significant knockdown of Mre11 and ATM mRNA (84% compared to DC+PEI-shControl) in vivo using in vivo-JetPEI (Supplementary Fig. 4A). The intensity and the number of γH2Ax+ pixels/cell as a measure of double strand breaks, were also significantly attenuated after DC+PEI-shMre11 and DC+PEI-shATM treatment indicating that knockdown of Mre11 and ATM reduces double strand breaks (Supplementary Fig. 4B and C). Electrophysiology across the lesion site after treatment with shMre11 and shATM demonstrated similar improvements in CAP amplitude (Supplementary Fig. 4D) and CAP area (Supplementary Fig. 4E) as observed after treatment with mirin and KU-60019. The mean sensing and removal time (Supplementary Fig. 4F) and mean ratio of slips to total steps (Supplementary Fig. 4G) in the sensory and locomotor tests were also similarly improved, with animals also being indistinguishable from sham-treated animals by 3 weeks, when compared to mirin and KU-60019 treatment. These results demonstrate that knockdown of Mre11 and ATM as well as mirin and KU-60019 treatment to inhibit the DNA damage response is functionally beneficial after spinal cord injury.
Discussion
Double strand breaks occur in both long-term and acute forms of neurological disease, including Alzheimer’s, Parkinson’s and Huntington’s diseases, amyotrophic lateral sclerosis, post-cerebral ischaemia and following spinal cord injury: potentially they are a universal feature. If double strand breaks accumulate in neurological conditions, attenuating the DNA damage response as a therapeutic strategy logically should enhance disease rather than suppress it. Here, we demonstrate the opposite is true: inhibiting apical components of the DNA damage response to mute the response to double strand breaks is beneficial in models of neurodegeneration and results in unprecedented survival, axon regeneration and recovery of function after neurotrauma.
Attenuating the DNA damage response in neurodegenerative disease
The incidence of double strand breaks in the brains of AD patients has only recently been quantified. Double strand breaks accumulate in early-stage AD and correlate with reduced cognitive scores (Simpson et al., 2015). It is plausible that these un-repaired double strand breaks trigger the cell-cycle re-entry phenomena in AD brains described in the 1990s (McShea et al., 1997; Nagy et al., 1997) and the increase in senescence identified more recently (He et al., 2013; Wei et al., 2016). Consistent with cell cycle re-entry affecting neural health and acting as trigger for apoptosis, inhibitors of cell cycle progression have shown some efficacy in cerebral ischemia models (Osuga et al., 2000) and have been suggested as therapies in neurodegeneration (reviewed in (Kruman and Schwartz, 2008)). Similarly caffeine, a non-specific inhibitor of ATM, is neuroprotective against etoposide-induced DNA damage to neurons in vitro (Kruman et al., 2004) and reducing ATM gene dosage is neuroprotective in a mouse model of HD (Lu et al., 2014). However, ATM inhibitors have not yet been tested in trials for neurodegeneration. In addition to double strand breaks, ATM is activated by multiple inputs, including reactive oxygen species, has a reported 700 potential downstream phosphorylation targets in multiple pathways. In contrast, the MRN complex has no reported functions other than in DNA damage repair, which potentially makes it a simpler target for therapy.
Would long-term targeting of the MRN complex as a therapeutic strategy for neurodegeneration cause cancer? Potentially, yes. However, heterozygous carriers of mutations in Mre11, Rad50, Nbs1/Nbn have only a small increase in cancer incidence over the lifecourse (Bartkova et al., 2008; Kuusisto et al., 2011; Damiola et al., 2014). This suggests that targeting the MRN complex may a feasible strategy, particular for more rapidly progressing neurodegenerative disorders such as ALS. Potentially, a genetic approach to partially knockdown MRN complex levels via shRNA would be more effective and could be largely restricted to the CNS using viral delivery.
Attenuating the DNA damage response in optic nerve injury
Clinically, the optic nerve crush injury model is directly relevant to glaucoma and other eye diseases where RGC death occurs such as ischemic optic neuropathy. For example, we previously evaluated the effect of an siRNA to caspase-2 (siCASP2) in protecting RGC from death in the same model used in this paper (Ahmed et al., 2011; Vigneswara et al., 2014). The siCASP2 is currently in Phase III clinical trials for the treatment of non-arteritic ischemic optic neuropathy as a result of our preclinical work (Ahmed et al., 2011); http://www.eyeactnow.com). Attenuation of the DNA damage response after optic nerve crush injury in vivo using Mre11 or ATM inhibitors significantly neuroprotected RGC from death. RGC neuroprotection was >90% after inhibition of mirin and KU-60019, which is on a par with the inhibition of caspase-2 using an siRNA (siCASP2) (Ahmed et al., 2011; Vigneswara and Ahmed, 2016). Out of all of the strategies to promote RGC neuroprotection, siCASP2 treatment appears to be the most effective (Thomas et al., 2017). Other treatments such as lens injury and cAMP+oncomudulin only protect to a maximum of 24% of RGC at 21 days after ONC (Leon et al., 2000; Yin et al., 2006). Even combinatorial treatments such as PTEN deletion + zymosan + cAMP only protected a maximum of 36% of RGC at 10-12 weeks after ONC (de Lima et al., 2012). The delivery of CNTF, a well-studied neurotrophic factor for RGC, promoted <60% RGC survival at 2 weeks (Pernet et al., 2013), declining to 31% at 5 weeks (Leaver et al., 2006) and 20% at 8 weeks (Pernet et al., 2013) after ONC. In contrast, twice weekly injections of mirin and KU-60019 neuroprotected >90% of RGC at 24 days after ONC. Although we have not studied time-points beyond 24 days, we would expect that this level of neuroprotection would continue as we have observed for siCASP2 for 12 weeks after ONC (Vigneswara and Ahmed, 2016). Therefore, inhibition of Mre11 and ATM to our knowledge is on par with the current best treatment for RGC neuroprotection, namely siCASP2.
Importantly, although siCASP2 offers >90% RGC neuroprotection, it does not promote RGC axon regeneration (Vigneswara et al., 2014). In contrast, Mre11 and ATM inhibitors not only promoted significant RGC survival, but also promoted significant RGC axon regeneration. Other treatments such lens injury and cAMP+oncomodulin only promote the regeneration of <1000 RGC axons at 1mm from the lesion at 21 days after ONC (Leon et al., 2000; Yin et al., 2006). Zymosan+cAMP and PTEN deletion+Zymosan+cAMP only promote the regeneration of <100 axons at 3mm from the lesion site at 2 weeks after ONC (Kurimoto et al., 2010; de Lima et al., 2012). Meanwhile, CNTF only promotes the regeneration of <20 axons at 1.5mm beyond the lesion site after 2 weeks and <50 axons at 4mm beyond the lesion site after 8 weeks (Leaver et al., 2006; Pernet et al., 2013). Our results showing robust RGC axon regeneration of >1000 axons at 4mm beyond the lesion site suggest that inhibition of Mre11 and ATM supersedes some of the best treatments that have been reported to date to promote RGC axon regeneration. Moreover, our results demonstrate that mirin or KU-60019 on their own cannot only protect RGC from death but also promote their axons to regenerate, making translation to the clinical scenario easier due to the requirement of delivering a single molecule to activate both neuroprotective and axon regenerative pathways.
Attenuating the DNA damage response in SCI
Attenuation of the DNA damage response after DC injury using Mre11 or ATM inhibitors promoted significant DC axon regeneration and electrophysiological, sensory and locomotor improvements. This is despite the presence of spinal cord cavities that are characteristic of DC crush injury in the rat. However, after DC injury, DRGN do not die and hence neuroprotection in vivo could not be assessed. Nonetheless, in vitro experiments with both Mre11 and ATM inhibitors demonstrated significant DRGN neuroprotection and hence we can surmise that the inhibitors are DRGN neuroprotective. The recovery of function after SCI in adult rats administered Mre11 or ATM inhibitors and confirmed using shMre11 and shATM, is striking and, to the best of our knowledge, unprecedented. The improvements in sensory and locomotor function that we achieved are perhaps more remarkable given that DC injury in adult rats results in spinal cord cavities but cavitation is absent after DC injury in the mouse (Surey et al., 2014). Instead in the mouse, the lesion sites fill with dense fibrous connective tissues which allow some regeneration of axons (Bilgen et al., 2007). In contrast, cavitation in the rat results in worsening of the initial injury, leading to disconnection of more axons over a period of several months (Tang et al., 2003; Byrnes et al., 2010).
Blunt injury, such as compression or contusion, account for more than >80% of all SCI in humans (Taoka and Okajima, 1998; Metz et al., 2000; Brechtel et al., 2006). Our rat DC crush model replicates many aspects of the human pathology well, including evolution of the injury over time and morphological aspects, such as spinal cord atrophy, myelomalacia, cavity, cyst and syrinx formation and cord disruption (Gruner, 1992; Taoka and Okajima, 1998; Kwon et al., 2002). Importantly, cavity and cysts form and increase over time, thus transecting more axons and causing ongoing damage to the spinal cord architecture. Our study, therefore, addresses axon regeneration and functional recovery in a model that cavitates, as opposed to mouse models where cavitation is absent. Taken together, these clinically relevant features suggest our approach could be a very effective therapy for patients.
The Mre11 and ATM inhibitors used in this study are potent, small molecule inhibitors that are specific for their targets and have a low IC50. These inhibitors were developed has chemotherapy agents for cancer. DNA damage-induced tumour cell killing has been demonstrated in preclinical models with mirin and KU-60019 (Hosoya and Miyagawa, 2014) and other ATM inhibitors are currently in trials that potentially could be re-purposed for SCI. Importantly, our method of administering the compounds – by intrathecal injection – can be translated directly to human therapy, This method gives direct access of compounds to the injury site, which often requires lower drug doses with greater efficiency than other routes of administration (Bottros and Christo, 2014), limiting the potential unwanted side effects on other cells in the CNS such as glia.
In our experiments, the mirin and KU-60019 compounds were delivered directly after injury but this could also happen in human patients since most cases attend emergency care immediately. For neurodegeneration, a viral-mediated genetic knockdown approach may prove to be more effective by effecting a partial knockdown in MRN complex levels over a longer period; this may also prove to be the case in SCI treatments. However, there are caveats to this approach. Inhibiting the DNA damage response may have negative consequences to cells and system functions if applied systemically. Even if used intrathecally as suggested by us, there may be negative consequences on dividing cells such as glia in the CNS. Hence, caution must be used when using these compounds in treating human conditions.
Current therapies for SCI are limited and only offer palliative relief with none reversing the underlying damage to axons. Methylprednisolone is the only pharmacotherapy that is currently approved for use in SCI, but this has failed to show clinically significant effects and is now often used off-label (Varma et al., 2013). Several other experimental therapies are currently in the early phases of development, including the Rho antagonist, Cethrin; anti-Nogo antibodies and autologous mesenchymal stem cell transplants. However, completed clinical trials of these reagents have either not been reported, abandoned or concluded with no significant improvements reported. Consequently, there is a clear unmet need for a therapy in SCI that would not only promote neuroprotection but also promote axon regeneration. It is clear from our previous work that neuroprotection and axon regeneration are signalled by different signalling pathways (Ahmed et al., 2010) and our study is the first to report single inhibitors capable of stimulating both processes. A ‘single hit’ molecule that can modify both neuroprotective and axon regenerative pathways would be an additional advantage for a potential therapy to treat the devastating loss of function that ensues after SCI in humans. Here, we report that DNA damage response inhibitors are good candidates.
In conclusion, our paper highlights a novel therapeutic strategy by targeting the response of neurons to double strand breaks to protect neurons from death and promote their axon regeneration. This strategy prevents loss of function in multiple neuropathological paradigms and suggests new treatment possibilities for neurological conditions.
Funding
This work was supported by a UK Biotechnology and Biological Sciences Research Council New Investigator award BB/N008472/1 (R.I.T.), AMA was supported by Marie-Curie ITN grant INsecTIME PITN-GA-2012-316790 (C.P.K), Saudi Education Ministry PhD Studentship (S.A. and Z.A.), University of Birmingham Bryant Bequest PhD Studentship (A.M.T. and Z.A.) and a UK Medical Research Council Confidence in Concept award (B.K). Author contributions: R.I.T., M.J.T., A.M.-A., S.C., A.J.N. and C.P.K. performed and analysed the Drosophila experiments; A.H.-A. and P.A. performed and analysed the hippocampal neuron experiments in collaboration with R.I.T.; J.L., A.M.T., S.A. and Z.A. performed and analysed the rat ex vivo and in vivo experiments; R.I.T., B.K. and Z.A. conceived the project; R.I.T., Z.A., C.J.K., B.K. and P.A. wrote the manuscript.
Competing interests
The authors report no competing interests.
Supplementary Figures
Supplementary Figure 1. Image processing of DNA double strand breaks in Drosophila neurons. 41 × 41 × 13.5 μm volumes of adult central brain neurons stained with anti-pH2Av were visualized by confocal microscopy with 0.45 m step sizes in z. A 63x N.A. 1.2 water immersion lens was used mounted on an inverted Zeiss LSM880 microscope and the Airy scan module used with super-resolution settings to improve resolution. Anti-pH2Av shows a uniform nuclear fluorescence both in our hands and in a previous report (Bellesi et al., 2016). Left panels show the projected z-series of images without adjustments to contrast or brightness. The right panels are the identical images processed to remove the low intensity fluorescence by resetting the black level to display only the bright pH2Av+ foci. Control neurons have a single pH2Av+ focus corresponding to the nucleolus organizing region. Arrowheads point to clusters of foci in neurons expressing Aβ1-42. Scale bars = 10µm.
Supplementary Figure 2. Genetic depletion of the MRN complex is neuroprotective in Drosophila neurodegeneration models. (A) Quantification of Tau.R406W expression in the heads of flies by Western blot. Flies were developed at 18 °C to inhibit expression of Tau then decapitated 0, 7, 14 or 21 d after shifting to 29 °C. Tau proteins levels are not altered in nbs−/+ heterozygotes. Phosphorylation of Tau at Thr181 is also unchanged. Quantification of Tau levels normalized to actin and the Tau:pTau ratio is shown below (n = 3; mean ± SEM; ANOVA with Dunn’s post-hoc test). (B) Negative geotaxis climbing assay. Flies expressing Htt.Q128 show premature decline in climbing ability. This is partially suppressed in flies heterozygous for a null rad50EP1 allele (p=0.0372) despite rad50 heterozygous flies already showing reduced climbing ability vs. the w1118 control (p=0.0007). Lines were fitted using non-linear regression and compared by extra sum-of-squares F-test. (C) Expression of Htt.Q128 in the eye leads to loss of pigment-containing cells in the retina after 7 weeks. Pigment loss is largely prevented in flies rad50−/+ flies. (D) and (E) Negative geotaxis climbing assays of flies expressing two forms of tandem Aβ1-42 expression with no flexible linker or with a 12-amino acid flexible linker (D) in adult neurons (Speretta et al., 2012). Flies climbed in vials with three marked zones. Aβ1-42 expressing (red) flies were compared to Aβ1-42; nbs1/+ flies (blue) on the days indicated after shifting to 29 °C to induce expression. Comparison was by χ2 test (* indicates P<0.05). (F) Circadian periodicity analyzed by cosinor analysis shows similar results to the CLEAN spectral analysis shown in Fig. 1F (Rosato and Kyriacou, 2006). Comparison by ANOVA with Tukey’s post-hoc test; p values and n are indicated.
Methods for Tau quantification in (A). 15 adult flies per genotype and time point were decapitated with a razor blade then homogenized in 150 μl RIPA buffer (New England Biolabs) supplemented with protease and phosphatase inhibitor cocktails (both Merck). Homogenization used a MP Biomedicals FastPrep blender running at 6.0 m/s for 20 s in screwcap tubes containing matrix C garnet beads (MP Biomedicals). Lysates were clarified by centrifugation at 12000 × g for 5 min at 4 °C then 6X Laemlli buffer + 50 mM DTT was added and the samples boiled for 5 min. Proteins were separated on 12% Tris.Glycine SDS-PAGE gels and transferred to PVDF membranes. Primary antibodies used were: rabbit anti-total Tau (1:1.000; DAKO); mouse anti-Tau pThr181 (1:500; ThermoFisher) and mouse IgM anti-actin JLA20 (1:5.000; Developmental Studies Hybridoma Bank). Secondary antibodies used were: HRP-conjugated horse anti-mouse IgG and HRP-conjugated horse anti-rabbit IgG (both 1:10,000; New England Biolabs; note: the anti-mouse IgG secondary antibody cross-reacts with mouse IgM). Membranes were visualized using a Vilber Fusion FX scanner. Total Tau levels were normalized to actin for each sample and phosphorylated Tau normalized to total Tau using ImageJ.
Supplementary Figure 3. Inhibition of Mre11 prevents DRGN apoptosis and stimulates neurite outgrowth in the presence of inhibitory CME. (A) Adult DRGN stained at 4 days after plating, with anti-γH2Ax antibodies to demonstrate double strand breaks (arrows) in DRGN in vitro, whilst satellite glial cells did not possess double strand breaks (arrowheads). (B) Representative images from DRGN after 4 days in culture and treated with vehicle, FGF2 (positive control), mirin and KU-60019. (C-E) Mirin or KU-60019 treatment enhances (C), DRGN survival, (D) % DRGN with neurites and (E) increased the mean length of the longest neurite. Comparison in (C-E) by one-way ANOVA with Dunnett’s post-hoc test, n = 3 wells/condition, 3 independent repeats (total n = 9 wells/condition (all 9 data points shown on graphs)). Scale bars in A = 100µm and B = 50µm.
Supplementary Figure 4. Intra-DRG injection of DNA plasmids to knock down Mre11 and ATM using non-viral in vivo-JetPEI (PEI) promotes significant functional repair after DC injury in vivo. (A) PEI delivered plasmids significantly knock down Mre11 and ATM mRNA expression in spinal L4/L5 DRGs at 4 weeks after DC injury. (B) Immunohistochemistry for γH2Ax to demonstrate DNA breaks 4 weeks after DC injury and their attenuation by shMre11 and shATM in DRGN. (C) Quantification of γH2Ax+ pixels/cells to demonstrate significant attenuation of double strand breaks by shMre11 and shATM. (D) Negative CAP amplitudes were significantly attenuated in DC+vehicle-treated rats but were significantly improved in DC+PEI-shMre11 and DC+PEI-shATM treatment (P<0.0001, one-way ANOVA (main effect)). (E) Mean CAP area at different stimulation intensities were significantly attenuated in DC+vehicle-treated rats but improved significantly in DC+PEI-shMre11 and DC+PEI-shATM-treated rats (P<0.0001, one-way ANOVA (main effect)). (F) Mean tape sensing and removal times are restored to normal 3 weeks after treatment with PEI-shMre11 and PEI-shATM (P<0.0001, independent sample t-test (DC+vehicle vs DC+PEI-shMre11/DC+PEI-shATM at 3 weeks) whilst a significant deficit remains in DC+vehicle-treated rats (# = P<0.00011, generalized linear mixed models over the whole 6 weeks). (G) Mean error ratio to show the number of slips vs. total number of steps in the horizontal ladder walking test also returns to normal 3 weeks after treatment with PEI-shMre11 and PEI-shATM (P<0.0001, independent sample t-test (DC+vehicle vs DC+PEI-shMre11/DC+PEI-shATM at 3 weeks)), with a deficit remaining in DC+vehicle-treated rats (## = P<0.00014, linear mixed models over the whole 6 weeks). AU = arbitrary units. n = 6 rats/treatment/test, 3 independent repeats (total n = 18 rats/treatment/test). Scale bars in B = 50µm, insets in B = 10µm.
Movies
Movie 1. Rotated views of 3D rendered volumes showing pH2Av+ foci in the nuclei of central brain neurons from control and Aβ1-42 expressing adult flies. Volumes shown in Fig. 1 and Supplementary Fig. 1 were rendered in Fiji-3. Control brain is to the left and Aβ1-42-expressing brain to the right.
Movie 2. Genetic reduction of nbs levels maintains neural activity in flies after 10 d of A expression. Flies expressing tandem Aβ1-42 (Speretta et al., 2012) specifically in adult neurons for 10 d under the control of Elav-Gal4. Gal80ts was included to prevent expression until eclosion. Flies were maintained at 18 °C during development and shifted to the permissive temperature of 29°C upon eclosion. Flies in the vial to the left are wild-type for nbs; flies to the right are heterozygous for the null nbs2 allele.
Acknowledgments
We thank Emma Richards for help with the initial stages of this project, Drs Damien Crowther and Mel Feany for providing Drosophila stocks and Professors Martin Berry and Malcolm Taylor for helpful comments on the manuscript.
Footnotes
Abbreviations AD = Alzheimer’s disease; ATM = ataxia telangiectasia mutated; ATR = ataxia telangiectasia and Radiation 3-related protein; BSA = bovine serum albumin; cAMP = cyclic adenosine monophosphate; CAP = compound action potential; CME = CNS myelin extracts; CMV = cytomegalovirus; CNTF = ciliary neurotrophic factor; Cy3 = cyanine 3; DAPI = 4’,6-diamidino-2-phenylindole; DC = dorsal column; DMEM = Dulbecco’s modified Eagle’s medium; DMSO = dimethyl sulfoxide; DN1 = dorsal neuron 1; DN2 = dorsal neuron 2; DRG = dorsal root ganglion; DRGN = dorsal root ganglion neurons; FBS = foetal bovine serum; FGF2 = fibroblast growth factor 2; GAP43 = growth associated protein 43; GLMM = generalised linear mixed models; HD = Huntington’s disease; HRP = horseradish peroxidase; IC50 = half maximum inhibitory concentration; IgG = Immunoglobulin; lLNv = large lateral neuron ventral; LMM = linear mixed models; LNd = lateral neuron dorsal; Mre11 = meiotic recombination 11; MRN complex = meiotic recombination 11/Radiation 50/Nijmegen breakage syndrome 1 complex; Nbs1 = Nijmegen breakage syndrome 1; NF = neurofilament; ONC = optic nerve crush; PAGE = polyacrylamide gel electrophoresis; PBS = phosphate buffered saline; PDF = pigment dispersing factor; PFA = paraformaldehyde; P/S = penicillin/streptomycin; PTEN = phosphatase and tensin homolog; RGC = retinal ganglion cells; Rad50 = Radiation 50; RT = room temperature; SCI = spinal cord injury; SDS = sodium dodecyl sulphate; shRNA = short hairpin RNA; siCASP2 = short interfering RNA to caspase-2; sLNv = small lateral neuron ventral; TBS = Tris buffered saline; UAS = upstream activation sequence.
Axon regeneration data in the optic system and spinal cord has been included in figures 4 and 6. Figure 1D was removed.