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In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex

Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, View ORCID ProfileEric Cascales, Martin Pilhofer, Eric Durand, View ORCID ProfileRémi Fronzes
doi: https://doi.org/10.1101/441683
Chiara Rapisarda
1CNRS UMR 5234 Microbiologie Fondamentale et Pathogénicité, Paris, France
2Institut Européen de Chimie et Biologie, University of Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France
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Yassine Cherrak
3Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie de la Méditerranée (IMM), UMR7255, Aix-Marseille Université - CNRS, Marseille, France
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Romain Kooger
4Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, CH-8093 Zürich, Switzerland
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Victoria Schmidt
3Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie de la Méditerranée (IMM), UMR7255, Aix-Marseille Université - CNRS, Marseille, France
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Riccardo Pellarin
5Institut Pasteur, Structural Bioinformatics Unit, Department of Structural Biology and Chemistry, CNRS UMR 3528, C3BI USR 3756, Paris, France
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Laureen Logger
3Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie de la Méditerranée (IMM), UMR7255, Aix-Marseille Université - CNRS, Marseille, France
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Eric Cascales
6Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie de la Méditerranée (IMM), UMR7255, INSERM - Marseille, France
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  • ORCID record for Eric Cascales
Martin Pilhofer
4Institute of Molecular Biology & Biophysics, Eidgenössische Technische Hochschule Zürich, CH-8093 Zürich, Switzerland
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  • For correspondence: pilhofer@biol.ethz.ch edurand@imm.cnrs.fr edurand@inserm.fr r.fronzes@iecb.u-bordeaux.fr
Eric Durand
3Laboratoire d’Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie de la Méditerranée (IMM), UMR7255, Aix-Marseille Université - CNRS, Marseille, France
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  • For correspondence: pilhofer@biol.ethz.ch edurand@imm.cnrs.fr edurand@inserm.fr r.fronzes@iecb.u-bordeaux.fr
Rémi Fronzes
1CNRS UMR 5234 Microbiologie Fondamentale et Pathogénicité, Paris, France
2Institut Européen de Chimie et Biologie, University of Bordeaux, 2 rue Robert Escarpit, 33607, Pessac, France
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  • For correspondence: pilhofer@biol.ethz.ch edurand@imm.cnrs.fr edurand@inserm.fr r.fronzes@iecb.u-bordeaux.fr
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Abstract

Bacteria have evolved macromolecular machineries that secrete effectors and toxins to survive and thrive in diverse environments. The type VI secretion system (T6SS) is a contractile machine that is related to Myoviridae phages. The T6SS is composed of a baseplate that contains a spike onto which an inner tube is built, surrounded by a contractile sheath. Unlike phages that are released to and act in the extracellular medium, the T6SS is an intracellular machine inserted in the bacterial membranes by a trans-envelope complex. This membrane complex (MC) comprises three proteins: TssJ, TssL and TssM. We previously reported the low-resolution negative stain electron microscopy structure of the enteroaggregative Escherichia coli MC and proposed a rotational 5-fold symmetry with a TssJ:TssL:TssM stoichiometry of 2:2:2. Here, cryo-electron tomography analysis of the T6SS MC confirmed the 5-fold symmetry in situ and identified the regions of the structure that insert into the bacterial membranes. A high resolution model obtained by single particle cryo-electron microscopy reveals its global architecture and highlights new features: five additional copies of TssJ, yielding a TssJ:TssL:TssM stoichiometry of 3:2:2, a 11-residue loop in TssM, protruding inside the lumen of the MC and constituting a functionally important periplasmic gate, and hinge regions. Based on these data, we revisit the model on the mechanism of action of the MC during T6SS assembly and function.

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Posted October 13, 2018.
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In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex
Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, Eric Cascales, Martin Pilhofer, Eric Durand, Rémi Fronzes
bioRxiv 441683; doi: https://doi.org/10.1101/441683
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In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex
Chiara Rapisarda, Yassine Cherrak, Romain Kooger, Victoria Schmidt, Riccardo Pellarin, Laureen Logger, Eric Cascales, Martin Pilhofer, Eric Durand, Rémi Fronzes
bioRxiv 441683; doi: https://doi.org/10.1101/441683

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