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In vivo assembly and trafficking of olfactory Ionotropic Receptors

Liliane Abuin, View ORCID ProfileLucia L. Prieto-Godino, View ORCID ProfileHaiyun Pan, Craig Gutierrez, Lan Huang, View ORCID ProfileRongsheng Jin, View ORCID ProfileRichard Benton
doi: https://doi.org/10.1101/441782
Liliane Abuin
1Center for Integrative Genomics Génopode Building Faculty of Biology and Medicine University of Lausanne CH-1015 Lausanne Switzerland
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Lucia L. Prieto-Godino
1Center for Integrative Genomics Génopode Building Faculty of Biology and Medicine University of Lausanne CH-1015 Lausanne Switzerland
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  • ORCID record for Lucia L. Prieto-Godino
Haiyun Pan
2Department of Physiology and Biophysics University of California, Irvine California 92697 USA
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Craig Gutierrez
2Department of Physiology and Biophysics University of California, Irvine California 92697 USA
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Lan Huang
2Department of Physiology and Biophysics University of California, Irvine California 92697 USA
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Rongsheng Jin
2Department of Physiology and Biophysics University of California, Irvine California 92697 USA
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Richard Benton
1Center for Integrative Genomics Génopode Building Faculty of Biology and Medicine University of Lausanne CH-1015 Lausanne Switzerland
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  • For correspondence: Richard.Benton@unil.ch
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Abstract

lonotropic Receptors (IRs) are a large, divergent subfamily of ionotropic glutamate receptors(iGluRs), with roles in chemosensation, thermosensation and hygrosensation. Analogous to the synaptic targeting mechanisms of their iGluR ancestors, IRs are thought to form complexes of broadly-expressed co-receptors and selectively-expressed ‘tuning’ receptors to localise to sensory cilia. While tuning receptors’ extracellular ligand-binding domain (LBD) defines sensory specificity, the role of this domain in co-receptors is unclear. We identify a coreceptor-specific sequence in the LBD, which contains a single N-glycosylation site. Combining molecular genetic and cell biological analyses, we show that this site is dispensable for assembly of IR complexes in olfactory sensory neurons, but essential for endoplasmic reticulum exit of some,but not all, IR complexes. Our data reveal an important role for the IR co-receptor LBD in control of intracellular transport, provide novel insights into the stoichiometry and assembly of IR complexes, and uncover an unexpected heterogeneity in the trafficking regulation of this sensory receptor family.

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Posted October 12, 2018.
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In vivo assembly and trafficking of olfactory Ionotropic Receptors
Liliane Abuin, Lucia L. Prieto-Godino, Haiyun Pan, Craig Gutierrez, Lan Huang, Rongsheng Jin, Richard Benton
bioRxiv 441782; doi: https://doi.org/10.1101/441782
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In vivo assembly and trafficking of olfactory Ionotropic Receptors
Liliane Abuin, Lucia L. Prieto-Godino, Haiyun Pan, Craig Gutierrez, Lan Huang, Rongsheng Jin, Richard Benton
bioRxiv 441782; doi: https://doi.org/10.1101/441782

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