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MicroRNAs buffer genetic variation at specific temperatures during embryonic development

View ORCID ProfileChristopher Amourda, Jeronica Chong, Timothy E. Saunders
doi: https://doi.org/10.1101/444810
Christopher Amourda
1Mechanobiology Institute, National University of Singapore, T-Lab, 5A Engineering Drive 1, Singapore 117411
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  • For correspondence: christopher.amourda@nus.edu.sg dbsste@nus.edu.sg
Jeronica Chong
1Mechanobiology Institute, National University of Singapore, T-Lab, 5A Engineering Drive 1, Singapore 117411
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Timothy E. Saunders
1Mechanobiology Institute, National University of Singapore, T-Lab, 5A Engineering Drive 1, Singapore 117411
2Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543
3Institute for Molecular and Cell Biology, Agency for Science Technology and Research, 61 Biopolis Dr, Singapore 138673
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  • For correspondence: christopher.amourda@nus.edu.sg dbsste@nus.edu.sg
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Abstract

Successful embryogenesis requires the coordination of developmental events. Perturbations, such as environmental changes, must be buffered to ensure robust development. However, how such buffering occurs is currently unknown in most developmental systems. Here, we demonstrate that seven miRNAs are differentially expressed during Drosophila embryogenesis at varying temperatures within natural physiological ranges. Lack of miR-3-309, -31a, -310c, -980 or -984c causes developmental delays specifically at a given temperature. Detailed analysis on miR-310c and -984c shows that their targets are typically mis-expressed in mutant backgrounds, with phenotypes more pronounced at temperatures where miRNAs show highest expression in wild-type embryos. Our results show that phenotypes may arise at specific temperatures while remaining silent at others, even within typical temperature ranges. Our work uncovers that miRNAs mask genetic variation at specific temperatures to increase embryonic robustness, highlighting another layer of complexity in miRNA expression.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 16, 2018.
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MicroRNAs buffer genetic variation at specific temperatures during embryonic development
Christopher Amourda, Jeronica Chong, Timothy E. Saunders
bioRxiv 444810; doi: https://doi.org/10.1101/444810
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MicroRNAs buffer genetic variation at specific temperatures during embryonic development
Christopher Amourda, Jeronica Chong, Timothy E. Saunders
bioRxiv 444810; doi: https://doi.org/10.1101/444810

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