Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Fast two-photon volumetric imaging of an improved voltage indicator reveals electrical activity in deeply located neurons in the awake brain

View ORCID ProfileMariya Chavarha, View ORCID ProfileVincent Villette, Ivan K. Dimov, Lagnajeet Pradhan, Stephen W. Evans, Dongqing Shi, Renzhi Yang, Simon Chamberland, Jonathan Bradley, Benjamin Mathieu, View ORCID ProfileFrancois St-Pierre, Mark J. Schnitzer, Guoqiang Bi, Katalin Toth, View ORCID ProfileJun Ding, Stéphane Dieudonné, View ORCID ProfileMichael Z. Lin
doi: https://doi.org/10.1101/445064
Mariya Chavarha
1Department of Bioengineering, Stanford University, Stanford, California, USA
2Department of Neurobiology, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mariya Chavarha
Vincent Villette
3Institut de Biologie de l′École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Vincent Villette
Ivan K. Dimov
2Department of Neurobiology, Stanford University, Stanford, California, USA
4CNC Program, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lagnajeet Pradhan
2Department of Neurobiology, Stanford University, Stanford, California, USA
4CNC Program, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephen W. Evans
2Department of Neurobiology, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dongqing Shi
2Department of Neurobiology, Stanford University, Stanford, California, USA
5School of Life Sciences, University of Science and Technology of China, Hefei, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Renzhi Yang
6Department of Neurosurgey, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Simon Chamberland
7Department of Psychiatry and Neuroscience, Quebec Mental Health Institute, Université Laval, Québec, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonathan Bradley
3Institut de Biologie de l′École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin Mathieu
3Institut de Biologie de l′École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Francois St-Pierre
8Baylor College of Medicine, Houston, Texas, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Francois St-Pierre
Mark J. Schnitzer
4CNC Program, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guoqiang Bi
5School of Life Sciences, University of Science and Technology of China, Hefei, China
9CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katalin Toth
7Department of Psychiatry and Neuroscience, Quebec Mental Health Institute, Université Laval, Québec, Canada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jun Ding
6Department of Neurosurgey, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Jun Ding
Stéphane Dieudonné
3Institut de Biologie de l′École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, PSL Research University, Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael Z. Lin
1Department of Bioengineering, Stanford University, Stanford, California, USA
2Department of Neurobiology, Stanford University, Stanford, California, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Michael Z. Lin
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Imaging of transmembrane voltage deep in brain tissue with cellular resolution has the potential to reveal information processing by neuronal circuits in living animals with minimal perturbation. Multi-photon voltage imaging in vivo, however, is currently limited by speed and sensitivity of both indicators and imaging methods. Here, we report the engineering of an improved genetically encoded voltage indicator, ASAP3, which exhibits up to 51% fluorescence responses in the physiological voltage range, sub-millisecond activation kinetics, and full responsivity under two-photon illumination. We also introduce an ultrafast local volume excitation (ULOVE) two-photon scanning method to sample ASAP3 signals in awake mice at kilohertz rates with increased stability and sensitivity. ASAP3 and ULOVE allowed continuous single-trial tracking of spikes and subthreshold events for minutes in deep locations, with subcellular resolution, and with repeated sampling over multiple days. By imaging voltage in visual cortex neurons, we found evidence for cell type-dependent subthreshold modulation by locomotion. Thus, ASAP3 and ULOVE enable continuous high-speed high-resolution imaging of electrical activity in deeply located genetically defined neurons during awake behavior.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted October 17, 2018.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Fast two-photon volumetric imaging of an improved voltage indicator reveals electrical activity in deeply located neurons in the awake brain
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Fast two-photon volumetric imaging of an improved voltage indicator reveals electrical activity in deeply located neurons in the awake brain
Mariya Chavarha, Vincent Villette, Ivan K. Dimov, Lagnajeet Pradhan, Stephen W. Evans, Dongqing Shi, Renzhi Yang, Simon Chamberland, Jonathan Bradley, Benjamin Mathieu, Francois St-Pierre, Mark J. Schnitzer, Guoqiang Bi, Katalin Toth, Jun Ding, Stéphane Dieudonné, Michael Z. Lin
bioRxiv 445064; doi: https://doi.org/10.1101/445064
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Fast two-photon volumetric imaging of an improved voltage indicator reveals electrical activity in deeply located neurons in the awake brain
Mariya Chavarha, Vincent Villette, Ivan K. Dimov, Lagnajeet Pradhan, Stephen W. Evans, Dongqing Shi, Renzhi Yang, Simon Chamberland, Jonathan Bradley, Benjamin Mathieu, Francois St-Pierre, Mark J. Schnitzer, Guoqiang Bi, Katalin Toth, Jun Ding, Stéphane Dieudonné, Michael Z. Lin
bioRxiv 445064; doi: https://doi.org/10.1101/445064

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4116)
  • Biochemistry (8819)
  • Bioengineering (6522)
  • Bioinformatics (23467)
  • Biophysics (11796)
  • Cancer Biology (9215)
  • Cell Biology (13327)
  • Clinical Trials (138)
  • Developmental Biology (7439)
  • Ecology (11416)
  • Epidemiology (2066)
  • Evolutionary Biology (15155)
  • Genetics (10439)
  • Genomics (14046)
  • Immunology (9174)
  • Microbiology (22164)
  • Molecular Biology (8815)
  • Neuroscience (47585)
  • Paleontology (350)
  • Pathology (1429)
  • Pharmacology and Toxicology (2492)
  • Physiology (3731)
  • Plant Biology (8082)
  • Scientific Communication and Education (1437)
  • Synthetic Biology (2221)
  • Systems Biology (6039)
  • Zoology (1253)