ABSTRACT
Super-resolution optical microscopy has extended the spatial resolution of cell biology from the cellular level to the nanoscale, enabling the observation of the interactive behavior of single mitochondria and lysosomes. Quantitative parametrization of interaction between mitochondria and lysosomes under super-resolution optical microscopy, however, is currently unavailable, which has severely limited our understanding of the molecular machinery underlying mitochondrial functionality. Here, we introduce an M-value to quantitatively investigate mitochondria and lysosome contact (MLC) and mitophagy under structured illumination microscopy. We found that the M-value for an MLC is typically less than 0.4, whereas in mitophagy it ranges from 0.5 to 1.0. This system permits further investigation of the detailed molecular mechanism governing the interactive behavior of mitochondria and lysosomes.
Abbreviations
- CCCP
- carbonyl cyanide m-chlorophenyl hydrazone
- FWHM
- full-width at half-maximum
- LTR
- Lyso-Tracker Red
- MLC
- mitochondria and lysosome contact
- MTG
- Mito-Tracker Green
- SIM
- structured illumination microscopy
Footnotes
Contact: Peixue Ling, E-mail: mail: lpxsdf{at}163.com, Address: 44 Wenhuaxi Rd, School of Pharmaceutical Sciences, Shandong University, Jinan 250101, China.
Jun-Lin Guan, E-mail: guanjl{at}ucmail.uc.edu, Address: 3125 Eden Ave Address: 3125 Eden Ave, Department of Cancer Biology, University of Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
Jiajie Diao, E-mail: jiajie.diao{at}uc.edu, Address: 3125 Eden Ave, Department of Cancer Biology, University of 21 Cincinnati College of Medicine, Cincinnati, OH 45267, USA.
Abbreviations
- CCCP
- carbonyl cyanide m-chlorophenyl hydrazone
- FWHM
- full-width at half-maximum
- LTR
- Lyso-Tracker Red
- MLC
- mitochondria and lysosome contact
- MTG
- Mito-Tracker Green
- SIM
- structured illumination microscopy
