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MicroRNA-200c suppresses epithelial-mesenchymal transition of ovarian cancer by targeting cofilin-2

View ORCID ProfileXuechen Yu, Yuanzhen Zhang, Wei Zhang, Huijun Chen
doi: https://doi.org/10.1101/449587
Xuechen Yu
1Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University
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Yuanzhen Zhang
1Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University
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Wei Zhang
1Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University
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Huijun Chen
1Department of Gynaecology and Obstetrics, Zhongnan Hospital of Wuhan University
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  • For correspondence: karrel@sina.com
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Abstract

This study investigated the effects of microRNA-200c (miR-200c) and cofilin-2 (CFL2) in regulating epithelial-mesenchymal transition (EMT) in ovarian cancer. The level of miR-200c was lower in invasive SKOV3 cells than that in non-invasive OVCAR3 cells, whereas CFL2 showed the opposite trend. Bioinformatics analysis and dual-luciferase reporter gene assays indicated that CFL2 was a direct target of miR-200c. Furthermore, SKOV3 and OVCAR3 cells were transfected with miR-200c mimic or inhibitor, pCDH-CFL2 (CFL2 overexpression), or CFL2 shRNA (CFL2 silencing). MiR-200c inhibition and CFL2 overexpression resulted in elevated levels of both CFL2 and vimentin while reducing E-cadherin expression. They also increased ovarian cancer cell invasion and migration in vitro and in vivo and increased the tumor volumes. Conversely, miR-200c mimic and CFL2 shRNA exerted the opposite effects as those aforementioned. In addition, the effects of pCDH-CFL2 and CFL2 shRNA were reversed by the miR-200c mimic and inhibitor, respectively. This finding suggested that miR-200c could be a potential tumor suppressor by targeting CFL2 in the EMT process.

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Posted October 22, 2018.
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MicroRNA-200c suppresses epithelial-mesenchymal transition of ovarian cancer by targeting cofilin-2
Xuechen Yu, Yuanzhen Zhang, Wei Zhang, Huijun Chen
bioRxiv 449587; doi: https://doi.org/10.1101/449587
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MicroRNA-200c suppresses epithelial-mesenchymal transition of ovarian cancer by targeting cofilin-2
Xuechen Yu, Yuanzhen Zhang, Wei Zhang, Huijun Chen
bioRxiv 449587; doi: https://doi.org/10.1101/449587

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