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Spatial and Temporal Analysis of the Stomach and Small Intestinal Microbiota in Fasted Healthy Humans

View ORCID ProfileAnna Seekatz, View ORCID ProfileMatthew Schnizlein, View ORCID ProfileMark Koenigsknecht, Jason Baker, View ORCID ProfileWilliam Hasler, View ORCID ProfileBarry Bleske, View ORCID ProfileVincent Young, Duxin Sun
doi: https://doi.org/10.1101/450221
Anna Seekatz
University of Michigan;
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Matthew Schnizlein
University of Michigan;
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Mark Koenigsknecht
University of Michigan;
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Jason Baker
University of Michigan;
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William Hasler
University of Michigan;
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Barry Bleske
University of New Mexico-Albuquerque
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Vincent Young
University of Michigan;
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Duxin Sun
University of Michigan;
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  • For correspondence: duxins@med.umich.edu
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Abstract

Although the microbiota in the proximal gastrointestinal (GI) tract has been implicated in health and disease, much of these microbes remains understudied compared to the distal GI tract. This study characterized the microbiota across multiple proximal GI sites over time in healthy individuals. As part of a study of the pharmacokinetics of oral mesalamine administration, healthy, fasted volunteers (N=8; 10 observation periods total) were orally intubated with a four-lumen catheter with multiple aspiration ports. Samples were taken from stomach, duodenal, and multiple jejunal sites, sampling hourly (≤7 hours) to measure mesalamine (administered at t=0), pH, and 16S rRNA gene-based composition. We observed a predominance of Firmicutes across proximal GI sites, with significant variation compared to stool. The microbiota was more similar within individuals over time than between subjects, with the fecal microbiota being unique from that of the small intestine. The stomach and duodenal microbiota displayed highest intra-individual variability compared to jejunal sites, which were more stable across time. We observed significant correlations in the duodenal microbial composition with changes in pH; linear mixed models identified positive correlations with multiple Streptococcus operational taxonomic units (OTU) and negative correlations with multiple Prevotella and Pasteurellaceae OTUs. Few OTUs correlated with mesalamine concentration. The stomach and duodenal microbiota exhibited greater compositional dynamics compared to the jejunum. Short-term fluctuations in the duodenal microbiota was correlated with pH. Given the unique characteristics and dynamics of the proximal GI tract microbiota, it is important to consider these local environments in health and disease states.

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Posted October 26, 2018.
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Spatial and Temporal Analysis of the Stomach and Small Intestinal Microbiota in Fasted Healthy Humans
Anna Seekatz, Matthew Schnizlein, Mark Koenigsknecht, Jason Baker, William Hasler, Barry Bleske, Vincent Young, Duxin Sun
bioRxiv 450221; doi: https://doi.org/10.1101/450221
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Spatial and Temporal Analysis of the Stomach and Small Intestinal Microbiota in Fasted Healthy Humans
Anna Seekatz, Matthew Schnizlein, Mark Koenigsknecht, Jason Baker, William Hasler, Barry Bleske, Vincent Young, Duxin Sun
bioRxiv 450221; doi: https://doi.org/10.1101/450221

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