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PROCYCLIC Trypanosoma brucei CELL CYCLE is IMPAIRED at the G1 STAGE by the PRESENCE of POLY (ADP-RIBOSE) in the NUCLEUS

Mariana Schlesinger, Salomé C. Vilchez Larrea, Silvia H. Fernández Villamil
doi: https://doi.org/10.1101/452730
Mariana Schlesinger
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Ciudad Autónoma de Buenos Aires, Argentina. Phone number: +5411 4783-2871. Fax number: +5411 4786-8578.
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Salomé C. Vilchez Larrea
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Ciudad Autónoma de Buenos Aires, Argentina. Phone number: +5411 4783-2871. Fax number: +5411 4786-8578.
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Silvia H. Fernández Villamil
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular “Dr. Héctor N. Torres”, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, 1428 Ciudad Autónoma de Buenos Aires, Argentina. Phone number: +5411 4783-2871. Fax number: +5411 4786-8578.
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  • For correspondence: s.villamil@ingebi.conicet.gov.ar silvia.villamil@gmail.com
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Abstract

Previously we demonstrated that an excess of poly (ADP-ribose) in the nucleus makes procyclic parasites more sensitive to hydrogen peroxide. However, the effect of an altered-PAR metabolism under standard conditions has not been addressed yet. Here we have analyzed the behavior of the growth curve of transgenic parasites that present this phenotype and studied cell cycle progression in synchronized cultures by flow cytometry and immunofluorescence. We have demonstrated that an excess of nuclear poly (ADP-ribose) produces a delay in the G1 phase of the cell cycle. Moreover, for the first time we have shown that poly (ADP-ribose) occurs at specific points very close to the mature basal body, suggesting there could be a link between the kinetoplast and poly (ADP-ribose) metabolism.

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Posted October 25, 2018.
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PROCYCLIC Trypanosoma brucei CELL CYCLE is IMPAIRED at the G1 STAGE by the PRESENCE of POLY (ADP-RIBOSE) in the NUCLEUS
Mariana Schlesinger, Salomé C. Vilchez Larrea, Silvia H. Fernández Villamil
bioRxiv 452730; doi: https://doi.org/10.1101/452730
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PROCYCLIC Trypanosoma brucei CELL CYCLE is IMPAIRED at the G1 STAGE by the PRESENCE of POLY (ADP-RIBOSE) in the NUCLEUS
Mariana Schlesinger, Salomé C. Vilchez Larrea, Silvia H. Fernández Villamil
bioRxiv 452730; doi: https://doi.org/10.1101/452730

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