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Nuclease dead Cas9 is a programmable roadblock for DNA replication

Kelsey Whinn, Gurleen Kaur, Jacob S. Lewis, Grant Schauer, Stefan Müller, Slobodan Jergic, Hamish Maynard, Zhong Yan Gan, Matharishwan Naganbabu, Marcel P. Bruchez, Michael E. O’Donnell, Nicholas E. Dixon, Antoine M. van Oijen, Harshad Ghodke
doi: https://doi.org/10.1101/455543
Kelsey Whinn
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
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Gurleen Kaur
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
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Jacob S. Lewis
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
2Illawarra Health and Medical Research Institute, Wollongong, New South Wales 2522, Australia
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Grant Schauer
3Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
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Stefan Müller
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
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Slobodan Jergic
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
2Illawarra Health and Medical Research Institute, Wollongong, New South Wales 2522, Australia
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Hamish Maynard
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
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Zhong Yan Gan
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
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Matharishwan Naganbabu
4Department of Chemistry and Center for Nucleic Acids Science and Technology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
5Department of Biological Sciences and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
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Marcel P. Bruchez
4Department of Chemistry and Center for Nucleic Acids Science and Technology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
5Department of Biological Sciences and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
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Michael E. O’Donnell
3Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
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Nicholas E. Dixon
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
2Illawarra Health and Medical Research Institute, Wollongong, New South Wales 2522, Australia
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Antoine M. van Oijen
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
2Illawarra Health and Medical Research Institute, Wollongong, New South Wales 2522, Australia
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Harshad Ghodke
1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
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Article Information

doi 
https://doi.org/10.1101/455543
History 
  • October 29, 2018.

Article Versions

  • Version 1 (October 29, 2018 - 08:51).
  • You are viewing Version 2, the most recent version of this article.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.

Author Information

  1. Kelsey Whinn1,*,
  2. Gurleen Kaur1,*,
  3. Jacob S. Lewis1,2,
  4. Grant Schauer3,
  5. Stefan Müller1,
  6. Slobodan Jergic1,2,
  7. Hamish Maynard1,
  8. Zhong Yan Gan1,
  9. Matharishwan Naganbabu4,5,
  10. Marcel P. Bruchez4,5,
  11. Michael E. O’Donnell3,
  12. Nicholas E. Dixon1,2,
  13. Antoine M. van Oijen1,2,‡ and
  14. Harshad Ghodke1,‡
  1. 1School of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, New South Wales 2522, Australia
  2. 2Illawarra Health and Medical Research Institute, Wollongong, New South Wales 2522, Australia
  3. 3Howard Hughes Medical Institute, Rockefeller University, New York, NY 10065, USA
  4. 4Department of Chemistry and Center for Nucleic Acids Science and Technology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
  5. 5Department of Biological Sciences and Molecular Biosensor and Imaging Center, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pennsylvania 15213, USA
  1. ↵‡Denotes corresponding author
  1. ↵* Denotes equal contribution

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Nuclease dead Cas9 is a programmable roadblock for DNA replication
Kelsey Whinn, Gurleen Kaur, Jacob S. Lewis, Grant Schauer, Stefan Müller, Slobodan Jergic, Hamish Maynard, Zhong Yan Gan, Matharishwan Naganbabu, Marcel P. Bruchez, Michael E. O’Donnell, Nicholas E. Dixon, Antoine M. van Oijen, Harshad Ghodke
bioRxiv 455543; doi: https://doi.org/10.1101/455543
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Nuclease dead Cas9 is a programmable roadblock for DNA replication
Kelsey Whinn, Gurleen Kaur, Jacob S. Lewis, Grant Schauer, Stefan Müller, Slobodan Jergic, Hamish Maynard, Zhong Yan Gan, Matharishwan Naganbabu, Marcel P. Bruchez, Michael E. O’Donnell, Nicholas E. Dixon, Antoine M. van Oijen, Harshad Ghodke
bioRxiv 455543; doi: https://doi.org/10.1101/455543

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