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Live cell bioimaging with carbon dots produced in situ by femtosecond laser from intracellular material

View ORCID ProfileAleksander M. Shakhov, Artyom A. Astafiev, View ORCID ProfileAlina A. Osychenko, Maria S. Syrchina, View ORCID ProfileViktor A. Nadtochenko
doi: https://doi.org/10.1101/455808
Aleksander M. Shakhov
Semenov Institute of Chemical Physics of RAS
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  • For correspondence: physics2007@yandex.ru
Artyom A. Astafiev
Semenov Institute of Chemical Physics of RAS
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Alina A. Osychenko
Semenov Institute of Chemical Physics of RAS
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Maria S. Syrchina
Semenov Institute of Chemical Physics of RAS
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Viktor A. Nadtochenko
Semenov Institute of Chemical Physics of RAS
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  • ORCID record for Viktor A. Nadtochenko
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Abstract

Owning to excellent optical properties and high biocompatibility carbon dots (CDs) have drawn increasing attention and have been widely applied as imaging agents for various bio-applications. Here we report a strategy for live-cell fluorescent bioimaging based on in situ synthesis of CDs within cells by tightly focused femtosecond laser pulses. Laser-produced carbon dots exhibit bright excitation-dependent fluorescence and are highly two-photon active under near infrared femtosecond excitation, thus demonstrating a potential for two-photon fluorescence imaging. The Raman spectra of fluorescent centers show strong D (1350 cm-1) and G (1590 cm-1) bands, thus suggesting that they are composed of carbon dots with sp2-hybridized core. Using Mouse GV oocytes as a model system we examine cytotoxicity and demonstrate the possibility of long-term fluorescent intracellular tracking of the laser-produced CDs. Created virtually in any point of the live cell, CD-based fluorescent μm-sized markers demonstrate high structural stability and retain bright fluorescence many hours after formation. Our results point to laser-produced fluorescent CDs as a highly-potent tool for cell cycle tracking, culture cell marking and probing intracellular movements.

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Posted January 09, 2019.
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Live cell bioimaging with carbon dots produced in situ by femtosecond laser from intracellular material
Aleksander M. Shakhov, Artyom A. Astafiev, Alina A. Osychenko, Maria S. Syrchina, Viktor A. Nadtochenko
bioRxiv 455808; doi: https://doi.org/10.1101/455808
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Live cell bioimaging with carbon dots produced in situ by femtosecond laser from intracellular material
Aleksander M. Shakhov, Artyom A. Astafiev, Alina A. Osychenko, Maria S. Syrchina, Viktor A. Nadtochenko
bioRxiv 455808; doi: https://doi.org/10.1101/455808

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