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Precise optical probing of perceptual detection

Gilad M. Lerman, Jonathan V. Gill, Dmitry Rinberg, Shy Shoham
doi: https://doi.org/10.1101/456764
Gilad M. Lerman
1Neuroscience Institute, New York University Langone Health, New York, NY, 10016. USA
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Jonathan V. Gill
1Neuroscience Institute, New York University Langone Health, New York, NY, 10016. USA
2Center for Neural Science, New York University, New York, NY, 10016. USA
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Dmitry Rinberg
1Neuroscience Institute, New York University Langone Health, New York, NY, 10016. USA
2Center for Neural Science, New York University, New York, NY, 10016. USA
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  • For correspondence: rinberg@nyu.edu shoham@nyu.edu
Shy Shoham
1Neuroscience Institute, New York University Langone Health, New York, NY, 10016. USA
3Tech4Health Institute, New York University Langone Health, New York, NY, 10016. USA
4Department of Ophthalmology, New York University Langone Health, New York, NY, 10016. USA
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  • For correspondence: rinberg@nyu.edu shoham@nyu.edu
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Summary

Establishing causal links between patterns of neuronal activity and perception is crucial for understanding brain function. Electrical and optogenetic stimulation experiments demonstrated that animals can detect activation of a few neurons. However, these methodologies offer very limited control of ensemble activity and yielded highly divergent thresholds. Here, we use holographic two-photon (2P) optogenetic stimulation to probe the detection of evoked neuronal activity at cellular and single action potential resolution, with millisecond precision. We find that mice can detect single action potentials evoked synchronously across <20 olfactory bulb neurons, while ruling out detection of indirect effects using a novel optical sham-photostimulation technique. Our results demonstrate that mice are acutely attuned to sparse, synchronous ensemble activity signals, introducing order-of-magnitude revisions to earlier estimates of perceptual thresholds.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted October 30, 2018.
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Precise optical probing of perceptual detection
Gilad M. Lerman, Jonathan V. Gill, Dmitry Rinberg, Shy Shoham
bioRxiv 456764; doi: https://doi.org/10.1101/456764
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Precise optical probing of perceptual detection
Gilad M. Lerman, Jonathan V. Gill, Dmitry Rinberg, Shy Shoham
bioRxiv 456764; doi: https://doi.org/10.1101/456764

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