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Extracellular electron uptake by two Methanosarcina species

View ORCID ProfileMon Oo Yee, Oona Snoeyenbos-West, View ORCID ProfileBo Thamdrup, Lars DM Ottosen, View ORCID ProfileAmelia-Elena Rotaru
doi: https://doi.org/10.1101/458091
Mon Oo Yee
1Nordcee, Department of Biology, University of Southern Denmark, Odense, Denmark
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Oona Snoeyenbos-West
1Nordcee, Department of Biology, University of Southern Denmark, Odense, Denmark
2Deparment of Engineering, University of Aarhus, Aarhus, Denmark
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Bo Thamdrup
1Nordcee, Department of Biology, University of Southern Denmark, Odense, Denmark
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Lars DM Ottosen
2Deparment of Engineering, University of Aarhus, Aarhus, Denmark
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Amelia-Elena Rotaru
1Nordcee, Department of Biology, University of Southern Denmark, Odense, Denmark
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  • ORCID record for Amelia-Elena Rotaru
  • For correspondence: arotaru@biology.sdu.dk
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Abstract

Direct electron uptake by prokaryotes is a recently described mechanism with a potential application for energy and CO2 storage into value added chemicals. Members of Methanosarcinales, an environmentally and biotechnologically relevant group of methanogens, were previously shown to retrieve electrons from an extracellular electrogenic partner performing Direct Interspecies Electron Transfer (DIET) and were therefore proposed to be electroactive. However, their intrinsic electroactivity has never been examined. In this study, we tested two methanogens belonging to Methanosarcina, M. barkeri and M. horonobensis, regarding their ability to accept electrons directly from insoluble electron donors like other cells, conductive particles and electrodes. Both methanogens were able to retrieve electrons from Geobacter metallireducens via DIET. Furthermore, DIET was also stimulated upon addition of electrically conductive granular activated carbon (GAC) when each was co-cultured with G. metallireducens. However, when provided with a cathode poised at – 400 mV (vs SHE), only M. barkeri could perform electromethanogenesis. In contrast, the strict hydrogenotrophic methanogen, Methanobacterium formicicum, did not produce methane regardless of the type of insoluble electron donor provided (Geobacter cells, GAC or electrodes). A comparison of functional gene categories between Methanobacterium and the two Methanosarcinas revealed a higher abundance of genes associated with extracellular electron transfer in Methanosarcina species. Between the two Methanosarcina we observed differences regarding energy metabolism, which could explain dissimilarities concerning electromethanogenesis at fixed potentials. We suggest that these dissimilarities are minimized in the presence of an electrogenic DIET partner (i.e. Geobacter), which can modulate its surface redox potentials by adjusting the expression of electroactive surface proteins.

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Posted October 31, 2018.
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Extracellular electron uptake by two Methanosarcina species
Mon Oo Yee, Oona Snoeyenbos-West, Bo Thamdrup, Lars DM Ottosen, Amelia-Elena Rotaru
bioRxiv 458091; doi: https://doi.org/10.1101/458091
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Extracellular electron uptake by two Methanosarcina species
Mon Oo Yee, Oona Snoeyenbos-West, Bo Thamdrup, Lars DM Ottosen, Amelia-Elena Rotaru
bioRxiv 458091; doi: https://doi.org/10.1101/458091

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