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Stem cell differentiation trajectories in Hydra resolved at single-cell resolution

View ORCID ProfileStefan Siebert, View ORCID ProfileJeffrey A. Farrell, View ORCID ProfileJack F. Cazet, View ORCID ProfileYashodara Abeykoon, View ORCID ProfileAbby S. Primack, View ORCID ProfileChristine E. Schnitzler, View ORCID ProfileCelina E. Juliano
doi: https://doi.org/10.1101/460154
Stefan Siebert
Department of Molecular and Cellular Biology, UC Davis, Davis, CA, USA;
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Jeffrey A. Farrell
Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, USA;
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Jack F. Cazet
Department of Molecular and Cellular Biology, UC Davis, Davis, CA, USA;
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Yashodara Abeykoon
Department of Molecular and Cellular Biology, UC Davis, Davis, CA, USA;
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Abby S. Primack
Department of Molecular and Cellular Biology, UC Davis, Davis, CA, USA;
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Christine E. Schnitzler
Whitney Laboratory for Marine Bioscience and Department of Biology, University of Florida, St. Augus
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Celina E. Juliano
Department of Molecular and Cellular Biology, UC Davis, Davis, CA, USA;
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  • ORCID record for Celina E. Juliano
  • For correspondence: cejuliano@ucdavis.edu
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Abstract

The adult Hydra polyp continuously renews all of its cells using three separate stem cell populations, but the genetic pathways enabling homeostatic tissue maintenance are not well understood. We used Drop-seq to sequence transcriptomes of 24,985 single Hydra cells and identified the molecular signatures of a broad spectrum of cell states, from stem cells to terminally differentiated cells. We constructed differentiation trajectories for each cell lineage and identified the transcription factors expressed along these trajectories, thus creating a comprehensive molecular map of all developmental lineages in the adult animal. We unexpectedly found that neuron and gland cell differentiation transits through a common progenitor state, suggesting a shared evolutionary history for these secretory cell types. Finally, we have built the first gene expression map of the Hydra nervous system. By producing a comprehensive molecular description of the adult Hydra polyp, we have generated a resource for addressing fundamental questions regarding the evolution of developmental processes and nervous system function.

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Posted November 03, 2018.
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Stem cell differentiation trajectories in Hydra resolved at single-cell resolution
Stefan Siebert, Jeffrey A. Farrell, Jack F. Cazet, Yashodara Abeykoon, Abby S. Primack, Christine E. Schnitzler, Celina E. Juliano
bioRxiv 460154; doi: https://doi.org/10.1101/460154
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Stem cell differentiation trajectories in Hydra resolved at single-cell resolution
Stefan Siebert, Jeffrey A. Farrell, Jack F. Cazet, Yashodara Abeykoon, Abby S. Primack, Christine E. Schnitzler, Celina E. Juliano
bioRxiv 460154; doi: https://doi.org/10.1101/460154

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