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Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences

View ORCID ProfileYaamini R. Venkataraman, View ORCID ProfileEmma Timmins-Schiffman, Micah J. Horwith, Alexander T. Lowe, View ORCID ProfileBrook Nunn, View ORCID ProfileBrent Vadopalas, Laura H. Spencer, View ORCID ProfileSteven B. Roberts
doi: https://doi.org/10.1101/460204
Yaamini R. Venkataraman
1School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98105, United States
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  • ORCID record for Yaamini R. Venkataraman
Emma Timmins-Schiffman
2Department of Genome Sciences, University of Washington, Seattle, Washington 98105, United States
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Micah J. Horwith
3Washington Department of Natural Resources, Olympia, Washington 98504, United States
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Alexander T. Lowe
4Department of Biology, University of Washington, Seattle, Washington 98105, United States
5Tennenbaum Marine Observatories Network, Smithsonian Institution, Edgewater, Maryland 21307, United States
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Brook Nunn
2Department of Genome Sciences, University of Washington, Seattle, Washington 98105, United States
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Brent Vadopalas
1School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98105, United States
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Laura H. Spencer
1School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98105, United States
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Steven B. Roberts
1School of Aquatic and Fishery Sciences, University of Washington, Seattle, Washington 98105, United States
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  • For correspondence: sr320@u.washington.edu
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Abstract

Global climate change is rapidly altering coastal marine ecosystems important for food production. A comprehensive understanding of how organisms will respond to these complex environmental changes can come only from observing and studying species within their natural environment. To this end, the effects of environmental drivers — pH, dissolved oxygen content, salinity, and temperature — on Pacific oyster (Crassostrea gigas) physiology were evaluated in an outplant experiment. Sibling juvenile oysters were outplanted to eelgrass and unvegetated habitat at five different estuarine sites within the Acidification Nearshore Monitoring Network in Washington State, USA to evaluate how regional environmental drivers influence molecular physiology. Within each site, we also determined if eelgrass presence that buffered pH conditions changed the oysters’ expressed proteome. A novel, two-step, gel-free proteomic approach was used to identify differences in protein abundance in C. gigas ctenidia tissue after a 29 day outplant by 1) identifying proteins in a data independent acquisition survey step and 2) comparing relative quantities of targeted environmental response proteins using selected reaction monitoring. While there was no difference in protein abundance detected between habitats or among sites within Puget Sound, C. gigas outplanted at Willapa Bay had significantly higher abundances of antioxidant enzymes and molecular chaperones. Environmental factors at Willapa Bay, such as higher average temperature, may have driven this protein abundance pattern. These findings generate a suite of new hypotheses for lab and field experiments to compare the effects of regional conditions on physiological responses of marine invertebrates.

Abbreviations
PS
Puget Sound
CI
Case Inlet
FB
Fidalgo Bay
PG
Port Gamble Bay
SK
Skokomish River Delta
WB
Willapa Bay
DIA
Data Independent Acquisition Mass Spectrometry
SRM
Selected Reaction Monitoring Assay
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 11, 2018.
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Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences
Yaamini R. Venkataraman, Emma Timmins-Schiffman, Micah J. Horwith, Alexander T. Lowe, Brook Nunn, Brent Vadopalas, Laura H. Spencer, Steven B. Roberts
bioRxiv 460204; doi: https://doi.org/10.1101/460204
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Characterization of Pacific oyster (Crassostrea gigas) proteomic response to natural environmental differences
Yaamini R. Venkataraman, Emma Timmins-Schiffman, Micah J. Horwith, Alexander T. Lowe, Brook Nunn, Brent Vadopalas, Laura H. Spencer, Steven B. Roberts
bioRxiv 460204; doi: https://doi.org/10.1101/460204

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