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Pectin digestion in herbivorous beetles: Impact of pseudoenzymes exceeds that of their active counterparts

Roy Kirsch, Grit Kunert, Heiko Vogel, Yannick Pauchet
doi: https://doi.org/10.1101/462531
Roy Kirsch
aDepartment of Entomology Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, Jena, 07745, Germany
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Grit Kunert
bDepartment of Biochemistry Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, Jena, 07745, Germany
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Heiko Vogel
aDepartment of Entomology Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, Jena, 07745, Germany
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Yannick Pauchet
aDepartment of Entomology Max Planck Institute for Chemical Ecology, Hans-Knoell-Str. 8, Jena, 07745, Germany
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Abstract

Many protein families harbor pseudoenzymes that have lost the catalytic function of their enzymatically active counterparts. Assigning alternative function and importance to these proteins is challenging [1]. Because the evolution towards pseudoenzymes is driven by gene duplication, they often accumulate in multigene families. Plant cell wall-degrading enzymes (PCWDEs) are prominent examples of expanded gene families. The pectolytic glycoside hydrolase family 28 (GH28) allows herbivorous insects to break down the PCW polysaccharide pectin. GH28 in the Phytophaga clade of beetles contains many active enzymes but also many inactive counterparts. Using functional characterization, gene silencing, global transcriptome analyses and recordings of life history traits, we found that not only catalytically active but also inactive GH28 proteins are part of the same pectin-digesting pathway. The robustness and plasticity of this pathway and thus its importance for the beetle is supported by extremely high steady-state expression levels and counter-regulatory mechanisms. Unexpectedly, the impact of pseudoenzymes on the pectin-digesting pathway in Phytophaga beetles exceeds even the influence of their active counterparts, such as a lowered efficiency of food-to-energy conversion and a prolongation of the developmental period.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 05, 2018.
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Pectin digestion in herbivorous beetles: Impact of pseudoenzymes exceeds that of their active counterparts
Roy Kirsch, Grit Kunert, Heiko Vogel, Yannick Pauchet
bioRxiv 462531; doi: https://doi.org/10.1101/462531
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Pectin digestion in herbivorous beetles: Impact of pseudoenzymes exceeds that of their active counterparts
Roy Kirsch, Grit Kunert, Heiko Vogel, Yannick Pauchet
bioRxiv 462531; doi: https://doi.org/10.1101/462531

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