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Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits

View ORCID ProfileKate Turner, Jacqueline Hoyle, Leonardo E Valdivia, Kara Cerveny, Wendy Hart, View ORCID ProfileMaryam Mangoli, Robert Geisler, Michele Rees, Corinne Houart, View ORCID ProfileRichard J. Poole, View ORCID ProfileStephen W Wilson, View ORCID ProfileGaia Gestri
doi: https://doi.org/10.1101/464123
Kate Turner
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
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  • ORCID record for Kate Turner
Jacqueline Hoyle
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
2Department of Paediatrics and Child Health, University College London, Gower St, London, WC1E 6BT, UK
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Leonardo E Valdivia
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
6Center for Integrative Biology, Facultad de Ciencias, Universidad Mayor, Santiago, Chile
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Kara Cerveny
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
3Department of Biology, Reed College, 3203 SE Woodstock Boulevard, Portland, OR 97202, USA
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Wendy Hart
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
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Maryam Mangoli
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
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  • For correspondence: g.gestri@ucl.ac.uk s.wilson@ucl.ac.uk
Robert Geisler
5Karlsruhe Institute of Technology (KIT) Institute of Toxicology and Genetics Hermann-von-Helmholtz-Platz 1 Building 439, Room 310 76344 Eggenstein-Leopoldshafen Germany
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Michele Rees
2Department of Paediatrics and Child Health, University College London, Gower St, London, WC1E 6BT, UK
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Corinne Houart
4Department of Developmental Neurobiology and MRC Centre for Developmental Disorders, Kings College London, SE1 1UL, UK
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Richard J. Poole
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
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Stephen W Wilson
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
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  • For correspondence: g.gestri@ucl.ac.uk s.wilson@ucl.ac.uk
Gaia Gestri
1Department of Cell and Developmental Biology, Division of Biosciences, University College London, Gower St, London, WC1E 6BT, UK
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  • For correspondence: g.gestri@ucl.ac.uk s.wilson@ucl.ac.uk
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Abstract

Through forward genetic screening for mutations affecting visual system development, we identified prominent coloboma and cell-autonomous retinal neuron differentiation, lamination and retinal axon projection defects in eisspalte (ele) mutant zebrafish. Additional axonal deficits were present, most notably at midline axon commissures. Genetic mapping and cloning of the ele mutation showed that the affected gene is slbp, which encodes a conserved RNA stem-loop binding protein involved in replication dependent histone mRNA metabolism. Cells throughout the central nervous system remained in the cell cycle in ele mutant embryos at stages when, and locations where, post-mitotic cells have differentiated in wild-type siblings. Indeed, RNAseq analysis showed down-regulation of many genes associated with neuronal differentiation. This was coincident with changes in the levels and spatial localisation of expression of various genes implicated, for instance, in axon guidance, that likely underlie specific ele phenotypes. These results suggest that many of the cell and tissue specific phenotypes in ele mutant embryos are secondary to altered expression of modules of developmental regulatory genes that characterise, or promote transitions in, cell state and require the correct function of Slbp-dependent histone and chromatin regulatory genes.

Author Summary Congenital deficits of eye formation are common in humans and to help understand the genetic basic of such conditions, we are studying zebrafish with comparable eye defects. We identified defects in both the shaping of the eye and in its connections to the brain in eisspalte mutant fish. Further analyses revealed additional deficits in the brain, most notably a severe reduction in neurons and their connections. We find that this is due to an inability of the cells that generate neurons to transition from proliferation to neuronal differentiation. By using a sequencing approach to compare mutant embryos to their normal siblings, we identified the affected gene as slbp, which encodes a protein that binds the mRNAs of other genes important for cell proliferation. This sequencing approach revealed the full extent of changes in gene expression in the mutant, helping us to better understand why the nervous system defects occur. Our study suggests that in the absence of Slbp function, cells lose the ability to transition from the proliferative to the differentiated state and this leads to additional defects in the eyes and brain.

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Posted November 06, 2018.
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Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits
Kate Turner, Jacqueline Hoyle, Leonardo E Valdivia, Kara Cerveny, Wendy Hart, Maryam Mangoli, Robert Geisler, Michele Rees, Corinne Houart, Richard J. Poole, Stephen W Wilson, Gaia Gestri
bioRxiv 464123; doi: https://doi.org/10.1101/464123
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Abrogation of Stem Loop Binding Protein (Slbp) function leads to a failure of cells to transition from proliferation to differentiation, retinal coloboma and midline axon guidance deficits
Kate Turner, Jacqueline Hoyle, Leonardo E Valdivia, Kara Cerveny, Wendy Hart, Maryam Mangoli, Robert Geisler, Michele Rees, Corinne Houart, Richard J. Poole, Stephen W Wilson, Gaia Gestri
bioRxiv 464123; doi: https://doi.org/10.1101/464123

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