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A stable, high refractive index, switching buffer for super-resolution imaging

Tobias M.P. Hartwich, Kenny Kwok Hin Chung, Lena Schroeder, Joerg Bewersdorf, Christian Soeller, David Baddeley
doi: https://doi.org/10.1101/465492
Tobias M.P. Hartwich
1Nanobiology Institute, Yale, New Haven, CT
2Department of Cell Biology, Yale, New Haven, CT
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Kenny Kwok Hin Chung
1Nanobiology Institute, Yale, New Haven, CT
2Department of Cell Biology, Yale, New Haven, CT
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Lena Schroeder
2Department of Cell Biology, Yale, New Haven, CT
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Joerg Bewersdorf
2Department of Cell Biology, Yale, New Haven, CT
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Christian Soeller
3Biomedical Physics, University of Exeter, Exeter, UK
4Department of Physiology, University of Auckland, Auckland, New Zealand
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David Baddeley
1Nanobiology Institute, Yale, New Haven, CT
2Department of Cell Biology, Yale, New Haven, CT
4Department of Physiology, University of Auckland, Auckland, New Zealand
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Abstract

dSTORM super-resolution imaging relies on switching buffers to enable dye molecules to enter and exit a metastable dark state. Current buffers have a very limited shelf life of approximately 1 day and poorly match sample refractive index, impacting negatively on measurement reproducibility and image fidelity. We present a buffer based on chemical, rather than enzymatic, oxygen scavenging which exhibits dramatically improved stability, switching speed, contrast, and index matching.

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Posted November 08, 2018.
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A stable, high refractive index, switching buffer for super-resolution imaging
Tobias M.P. Hartwich, Kenny Kwok Hin Chung, Lena Schroeder, Joerg Bewersdorf, Christian Soeller, David Baddeley
bioRxiv 465492; doi: https://doi.org/10.1101/465492
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A stable, high refractive index, switching buffer for super-resolution imaging
Tobias M.P. Hartwich, Kenny Kwok Hin Chung, Lena Schroeder, Joerg Bewersdorf, Christian Soeller, David Baddeley
bioRxiv 465492; doi: https://doi.org/10.1101/465492

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